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Physicochemical Characteristics, Entrapment Efficiency, and Stability of Nanostructured Lipid Carriers Loaded Coenzyme Q10 with Different Lipid Ratios

Yıl 2023, Cilt: 27 Sayı: 3, 1134 - 1142, 28.06.2025

Abdulloh Suyuti Esti Hendradi Tutiek Purwanti

Öz

Nanostructured Lipid Carriers (NLC) could be a good choice for dermal or transdermal delivery. It could dissolve lipophilic drugs, solve the problem of low skin permeation, and also be photoprotective. The composition of constituent materials and the ratio between solid lipid and liquid lipid influence the characteristics and stability of NLC. Compritol 888 ATO and Miglyol 812 have been widely studied for the development of dermal or transdermal preparation, but this combination has not been studied for use in Coenzyme Q10 loaded NLC systems. This study is to determine the effect of different lipid ratios of Compritol 888 ATO as solid lipid and Miglyol 812 as liquid lipid on the physicochemical characteristics, entrapment efficiency, and stability of NLC loaded Coenzyme Q10 using the High Shear Homogenization method. Three different lipid ratios were used in the NLC formulation, in which the ratio of Compritol 888 ATO : Miglyol 812 were 70:30, 80:20, and 90:10, respectively. NLC was characterized for FTIR spectra, differential scanning calorimetry, organoleptic, particle size, polydispersity index, zeta potential, pH, viscosity, entrapment efficiency, and evaluated for stability using the real-time method for 1 month. The result showed that the polydispersity index, zeta potential, and pH value were not significantly impacted by the various ratios of lipid. On the other hand, it had a considerable impact on three different formulas for particle size, viscosity, and entrapment efficiency. There were produced NLC with small particle size, low crystallinity, and high Coenzyme Q10 entrapment efficiency.

Anahtar Kelimeler

Coenzyme Q10 Nanostructured Lipid Carrier characterization entrapment efficiency stability

Kaynakça

  • [1] Addor FAS. Antioxidants in dermatology. An Bras Dermatol. 2017;92(3):356-362. [CrossRef]
  • [2] Andarina R, Djauhari T. Antioksidan dalam Dermatologi. J Kedokteran dan Kesehatan. 2017; 4(1): 39–48. [3] Hernández-Camacho JD, Bernier M, López-Lluch G, Navas P. Coenzyme Q10 supplementation in aging and disease. Front Physiol. 2018; 9(FEB): 1–11. [CrossRef]
  • [4] Ryu KA, Park PJ, Kim SB, Bin BH, Jang DJ, Kim ST. Topical delivery of coenzyme Q10-loaded microemulsion for skin regeneration. Pharmaceutics. 2020; 12(4): 1-15. [CrossRef]
  • [5] Guedes L de S, Martinez RM, Bou-Chacra NA, Velasco MVR, Rosado C, Baby AR. An overview on topical administration of carotenoids and coenzyme q10 loaded in lipid nanoparticles. Antioxidants. 2021; 10(7): 1–25. [CrossRef]
  • [6] Tessema EN, Bosse K, Wohlrab J, Mrestani Y, Neubert RHH. Investigation of ex vivo skin penetration of Coenzyme Q10 from microemulsions and hydrophilic cream. Skin Pharmacol Physiol. 2021; 33(6): 293–299. [CrossRef]
  • [7] Aburahma MH, Badr-Eldin SM. Compritol 888 ATO: A multifunctional lipid excipient in drug delivery systems and nanopharmaceuticals. Expert Opin Drug Deliv. 2014; 11(12): 1865–1883. [CrossRef]
  • [8] Mendes IT, Ruela ALM, Carvalho FC, Freitas JTJ, Bonfilio R, Pereira GR. Development and characterization of nanostructured lipid carrier-based gels for the transdermal delivery of donepezil. Colloids Surf B Biointerfaces. 2019; 177(July 2018): 274–281. [CrossRef]
  • [9] Nene S, Shah S, Rangaraj N, Mehra NK, Singh PK, Srivastava S. Lipid based nanocarriers: A novel paradigm for topical antifungal therapy. J Drug Deliv Sci Technol. 2020; 62(December 2020): 102397. [CrossRef]
  • [10] Tamjidi F, Shahedi M, Varshosaz J, Nasirpour A. Nanostructured lipid carriers (NLC): A potential delivery system for bioactive food molecules. Innov Food Sci Emerg Technol. 2013; 19: 29-43. [CrossRef]
  • [11] Nayak K, Katiyar SS, Kushwah V, Jain S. Coenzyme Q10 and retinaldehyde co-loaded nanostructured lipid carriers for efficacy evaluation in wrinkles. J Drug Target. 2017; 26(4): 333–344. [CrossRef]
  • [12] Gu Y, Tang X, Yang M, Yang D, Liu J. Transdermal drug delivery of triptolide-loaded nanostructured lipid carriers: Preparation, pharmacokinetic, and evaluation for rheumatoid arthritis. Int J Pharm. 2019; 554: 235-244. [CrossRef]
  • [13] Ortiz AC, Yañez O, Salas-Huenuleo E, Morales JO. Development of a nanostructured lipid carrier (NLC) by a lowenergy method, comparison of release kinetics and molecular dynamics simulation. Pharmaceutics. 2021; 13(4): 1-21. [CrossRef]
  • [14] Sznitowska M, Wolska E, Baranska H, Cal K, Pietkiewicz J. The effect of a lipid composition and a surfactant on the characteristics of the solid lipid microspheres and nanospheres (SLM and SLN). Eur J Pharm Biopharm. 2017; 110: 24–30. [CrossRef]
  • [15] Siafaka P, Okur ME, Ayla Ş, Er S, Cağlar EŞ, Okur NÜ. Design and characterization of nanocarriers loaded with levofloxacin for enhanced antimicrobial activity; physicochemical properties, in vitro release and oral acute toxicity. Braz J Pharm Sci., 2019; 55: 1–13. [CrossRef]
  • [16] Remya PN, Damodharan N. Formulation, development and characterisation of nimodipine loaded solid lipid nanoparticles. Int J Appl Pharm. 2020; 12(5): 265–271. [CrossRef]
  • [17] Shoviantari F, Erawati T, Soeratri W. Skin penetration of Coenzyme Q10 in nanostructure lipid carriers using olive oil and cetyl palmitate. Int J Pharm Clin Res. 2017; 9(2): 142-145. [18] Salvi VR, Pawar P. Nanostructured lipid carriers (NLC) system: A novel drug targeting carrier. J Drug Deliv Sci Technol. 2019; 51(990): 255–267. [CrossRef]
  • [19] Apostolou M, Assi S, Fatokun AA, Khan I. The effects of solid and liquid lipids on the physicochemical properties of nanostructured lipid carriers. J Pharm Sci. 2021; 110(8): 2859–2872. [CrossRef]
  • [20] Hendradi E, Rosita N, Rahmadhanniar E. Effect of lipid ratio of stearic acid and oleic acid on characteristics of nanostructure lipid carrier (NLC) system of diethylammonium diclofenac. Indones J Pharm. 2017; 28(4): 198–204. [CrossRef]
  • [21] Khater D, Nsairat H, Odeh F, Saleh M, Jaber A, Alshaer W, Al Bawab A, Mubarak MS. Design, preparation, and characterization of effective dermal and transdermal lipid nanoparticles : A review. Cosmetics. 2021; 8: 1–43. [CrossRef]
  • [22] Prakash C, Bhargave P, Tiwari S, Majumdar B, Bhargava RK. Skin surface pH in acne vulgaris: Insights from an observational study and review of the literature. J Clin Aesthet Dermatol. 2017; 10(7): 33–39. [23] Putranti AR, Primaharinastiti R, Hendradi E. Effectivity and physicochemical stability of nanostructured lipid carrier coenzyme Q10 in different ratio of lipid cetyl palmitate and alpha tocopheryl acetate as carrier. Asian J Pharm ClinRes. 2017; 10(2): 146–152. [CrossRef]

Yıl 2023, Cilt: 27 Sayı: 3, 1134 - 1142, 28.06.2025

Abdulloh Suyuti Esti Hendradi Tutiek Purwanti

Öz

Kaynakça

  • [1] Addor FAS. Antioxidants in dermatology. An Bras Dermatol. 2017;92(3):356-362. [CrossRef]
  • [2] Andarina R, Djauhari T. Antioksidan dalam Dermatologi. J Kedokteran dan Kesehatan. 2017; 4(1): 39–48. [3] Hernández-Camacho JD, Bernier M, López-Lluch G, Navas P. Coenzyme Q10 supplementation in aging and disease. Front Physiol. 2018; 9(FEB): 1–11. [CrossRef]
  • [4] Ryu KA, Park PJ, Kim SB, Bin BH, Jang DJ, Kim ST. Topical delivery of coenzyme Q10-loaded microemulsion for skin regeneration. Pharmaceutics. 2020; 12(4): 1-15. [CrossRef]
  • [5] Guedes L de S, Martinez RM, Bou-Chacra NA, Velasco MVR, Rosado C, Baby AR. An overview on topical administration of carotenoids and coenzyme q10 loaded in lipid nanoparticles. Antioxidants. 2021; 10(7): 1–25. [CrossRef]
  • [6] Tessema EN, Bosse K, Wohlrab J, Mrestani Y, Neubert RHH. Investigation of ex vivo skin penetration of Coenzyme Q10 from microemulsions and hydrophilic cream. Skin Pharmacol Physiol. 2021; 33(6): 293–299. [CrossRef]
  • [7] Aburahma MH, Badr-Eldin SM. Compritol 888 ATO: A multifunctional lipid excipient in drug delivery systems and nanopharmaceuticals. Expert Opin Drug Deliv. 2014; 11(12): 1865–1883. [CrossRef]
  • [8] Mendes IT, Ruela ALM, Carvalho FC, Freitas JTJ, Bonfilio R, Pereira GR. Development and characterization of nanostructured lipid carrier-based gels for the transdermal delivery of donepezil. Colloids Surf B Biointerfaces. 2019; 177(July 2018): 274–281. [CrossRef]
  • [9] Nene S, Shah S, Rangaraj N, Mehra NK, Singh PK, Srivastava S. Lipid based nanocarriers: A novel paradigm for topical antifungal therapy. J Drug Deliv Sci Technol. 2020; 62(December 2020): 102397. [CrossRef]
  • [10] Tamjidi F, Shahedi M, Varshosaz J, Nasirpour A. Nanostructured lipid carriers (NLC): A potential delivery system for bioactive food molecules. Innov Food Sci Emerg Technol. 2013; 19: 29-43. [CrossRef]
  • [11] Nayak K, Katiyar SS, Kushwah V, Jain S. Coenzyme Q10 and retinaldehyde co-loaded nanostructured lipid carriers for efficacy evaluation in wrinkles. J Drug Target. 2017; 26(4): 333–344. [CrossRef]
  • [12] Gu Y, Tang X, Yang M, Yang D, Liu J. Transdermal drug delivery of triptolide-loaded nanostructured lipid carriers: Preparation, pharmacokinetic, and evaluation for rheumatoid arthritis. Int J Pharm. 2019; 554: 235-244. [CrossRef]
  • [13] Ortiz AC, Yañez O, Salas-Huenuleo E, Morales JO. Development of a nanostructured lipid carrier (NLC) by a lowenergy method, comparison of release kinetics and molecular dynamics simulation. Pharmaceutics. 2021; 13(4): 1-21. [CrossRef]
  • [14] Sznitowska M, Wolska E, Baranska H, Cal K, Pietkiewicz J. The effect of a lipid composition and a surfactant on the characteristics of the solid lipid microspheres and nanospheres (SLM and SLN). Eur J Pharm Biopharm. 2017; 110: 24–30. [CrossRef]
  • [15] Siafaka P, Okur ME, Ayla Ş, Er S, Cağlar EŞ, Okur NÜ. Design and characterization of nanocarriers loaded with levofloxacin for enhanced antimicrobial activity; physicochemical properties, in vitro release and oral acute toxicity. Braz J Pharm Sci., 2019; 55: 1–13. [CrossRef]
  • [16] Remya PN, Damodharan N. Formulation, development and characterisation of nimodipine loaded solid lipid nanoparticles. Int J Appl Pharm. 2020; 12(5): 265–271. [CrossRef]
  • [17] Shoviantari F, Erawati T, Soeratri W. Skin penetration of Coenzyme Q10 in nanostructure lipid carriers using olive oil and cetyl palmitate. Int J Pharm Clin Res. 2017; 9(2): 142-145. [18] Salvi VR, Pawar P. Nanostructured lipid carriers (NLC) system: A novel drug targeting carrier. J Drug Deliv Sci Technol. 2019; 51(990): 255–267. [CrossRef]
  • [19] Apostolou M, Assi S, Fatokun AA, Khan I. The effects of solid and liquid lipids on the physicochemical properties of nanostructured lipid carriers. J Pharm Sci. 2021; 110(8): 2859–2872. [CrossRef]
  • [20] Hendradi E, Rosita N, Rahmadhanniar E. Effect of lipid ratio of stearic acid and oleic acid on characteristics of nanostructure lipid carrier (NLC) system of diethylammonium diclofenac. Indones J Pharm. 2017; 28(4): 198–204. [CrossRef]
  • [21] Khater D, Nsairat H, Odeh F, Saleh M, Jaber A, Alshaer W, Al Bawab A, Mubarak MS. Design, preparation, and characterization of effective dermal and transdermal lipid nanoparticles : A review. Cosmetics. 2021; 8: 1–43. [CrossRef]
  • [22] Prakash C, Bhargave P, Tiwari S, Majumdar B, Bhargava RK. Skin surface pH in acne vulgaris: Insights from an observational study and review of the literature. J Clin Aesthet Dermatol. 2017; 10(7): 33–39. [23] Putranti AR, Primaharinastiti R, Hendradi E. Effectivity and physicochemical stability of nanostructured lipid carrier coenzyme Q10 in different ratio of lipid cetyl palmitate and alpha tocopheryl acetate as carrier. Asian J Pharm ClinRes. 2017; 10(2): 146–152. [CrossRef]
Toplam 20 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular İlaç Dağıtım Teknolojileri
Bölüm Articles
Yazarlar

Abdulloh Suyuti 0000-0002-4782-9030

Esti Hendradi 0000-0002-8216-8549

Tutiek Purwanti

Yayımlanma Tarihi 28 Haziran 2025
Yayımlandığı Sayı Yıl 2023 Cilt: 27 Sayı: 3

Kaynak Göster

APA Suyuti, A., Hendradi, E., & Purwanti, T. (2025). Physicochemical Characteristics, Entrapment Efficiency, and Stability of Nanostructured Lipid Carriers Loaded Coenzyme Q10 with Different Lipid Ratios. Journal of Research in Pharmacy, 27(3), 1134-1142.
AMA Suyuti A, Hendradi E, Purwanti T. Physicochemical Characteristics, Entrapment Efficiency, and Stability of Nanostructured Lipid Carriers Loaded Coenzyme Q10 with Different Lipid Ratios. J. Res. Pharm. Haziran 2025;27(3):1134-1142.
Chicago Suyuti, Abdulloh, Esti Hendradi, ve Tutiek Purwanti. “Physicochemical Characteristics, Entrapment Efficiency, and Stability of Nanostructured Lipid Carriers Loaded Coenzyme Q10 With Different Lipid Ratios”. Journal of Research in Pharmacy 27, sy. 3 (Haziran 2025): 1134-42.
EndNote Suyuti A, Hendradi E, Purwanti T (01 Haziran 2025) Physicochemical Characteristics, Entrapment Efficiency, and Stability of Nanostructured Lipid Carriers Loaded Coenzyme Q10 with Different Lipid Ratios. Journal of Research in Pharmacy 27 3 1134–1142.
IEEE A. Suyuti, E. Hendradi, ve T. Purwanti, “Physicochemical Characteristics, Entrapment Efficiency, and Stability of Nanostructured Lipid Carriers Loaded Coenzyme Q10 with Different Lipid Ratios”, J. Res. Pharm., c. 27, sy. 3, ss. 1134–1142, 2025.
ISNAD Suyuti, Abdulloh vd. “Physicochemical Characteristics, Entrapment Efficiency, and Stability of Nanostructured Lipid Carriers Loaded Coenzyme Q10 With Different Lipid Ratios”. Journal of Research in Pharmacy 27/3 (Haziran 2025), 1134-1142.
JAMA Suyuti A, Hendradi E, Purwanti T. Physicochemical Characteristics, Entrapment Efficiency, and Stability of Nanostructured Lipid Carriers Loaded Coenzyme Q10 with Different Lipid Ratios. J. Res. Pharm. 2025;27:1134–1142.
MLA Suyuti, Abdulloh vd. “Physicochemical Characteristics, Entrapment Efficiency, and Stability of Nanostructured Lipid Carriers Loaded Coenzyme Q10 With Different Lipid Ratios”. Journal of Research in Pharmacy, c. 27, sy. 3, 2025, ss. 1134-42.
Vancouver Suyuti A, Hendradi E, Purwanti T. Physicochemical Characteristics, Entrapment Efficiency, and Stability of Nanostructured Lipid Carriers Loaded Coenzyme Q10 with Different Lipid Ratios. J. Res. Pharm. 2025;27(3):1134-42.