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The in vivo Activity of Fraxinus angustifolia in Pain and Inflammation- Examination of the Biological Activities of the Sub-extracts in Mice

Yıl 2023, Cilt: 27 Sayı: 3, 1252 - 1259, 28.06.2025

Yeşim Kaya Yaşar Serhat Sevgi Gülin Renda İrem Çavuşoğlu Nalbantoğlu Mine Duman Feride Sena Sezen

Öz

Fraxinus species (Oleaceae) are commonly used in the treatment of several inflammatory conditions with
pain, such as rheumatism, arthritis, and gout in folk medicine. We aimed to evaluate this ethnobotanical claim through
in vivo experiments. The n-hexane, dichloromethane, ethyl acetate and water subextracts were obtained from the
methanolic extract, which was prepared from the bark of Fraxinus angustifolia Vahl. The antinociceptive and antiinflammatory activities of extracts were evaluated in mice by hot plate and formalin-induced edema assays,
respectively. Mice were treated with 5-100 mg/kg methanol crude extract and 100 mg/kg subextracts intraperitoneally.
Administration of methanol extract decreased paw thickness in doses of 25 and 100 mg/kg. Water and ethyl acetate
subextracts decreased paw thickness while water subextract also decreased paw volume compared with the control.
Paw thickness values in the dichloromethane and n-hexane subextract groups and paw volume values in the ethyl
acetate, dichloromethane, and n-hexane subextract groups were comparable to those in the control group. The latency
values were higher in the groups treated with the dichloromethane and n-hexane subextracts than in the control group
while the other subextracts did not change the latency values compared to the control group in the hot plate test. In
conclusion, the cortex of Fraxinus angustifolia exhibited a significant in vivo anti-inflammatory and antinociceptive
activity.

Anahtar Kelimeler

Antinociceptive hot-plate formaline fraxinus ash

Kaynakça

  • [1] Caudullo G, Houston Durrant T. Fraxinus angustifolia in Europe: distribution, habitat, usage and threats. In: SanMiguel Ayanz J, de Rigo D, Caudullo G, Houston Durrant T, Mauri A. (Eds). European Atlas of Forest Tree Species. Luxembourg, Publication Office of the European Union, 2016, pp.97.
  • [2] Atmani D, Chaher N, Berboucha M, Ayouni K, Lounis H, Boudaoud H, Debbache N, Atmani D. Antioxidant capacity and phenol content of selected Algerian medicinal plants. Food Chem. 2009;112(2):303-309. https://doi.org/10.1016/j.foodchem.2008.05.077.
  • [3] Hamel T, Sadou S, Seridi R, Boukhdir S, Boulemtafes A. Pratique traditionnelle d’utilisation des plantes médicinales dans la population de la péninsule de l’Edough (nord-est algérien). Ethnopharmacologia 2018;59:65-71.
  • [4] Beloued A. Plantes Médicinales d’Algérie. Algiers. Algeria: Office des Publications Universitaires; 1998.
  • [5] Ayouni K, Berboucha-Rahmani M, Kim HK, Atmani D, Verpoorte R, Choi YH. Metabolomic tool to identify antioxidant compounds of Fraxinus angustifolia leaf and stem bark extracts. Ind Crop Prod. 2016;88:65-77. https://doi.org/10.1016/j.indcrop.2016.01.001.
  • [6] Yazıcıoğlu A, Tuzlacı E. Folk medicinal plants of Trabzon (Turkey). Fitoterapia 1996;67:307-318.
  • [7] Moulaoui K, Caddeo C, Manca ML, Castangia I, Valenti D, Escribano E, Atmani D, Fadda AM, Manconi M. Identification and nanoentrapment of polyphenolic phytocomplex from Fraxinus angustifolia: In vitro and in vivo wound healing potential. Eur J Med Chem. 2015;89:179-188. https://doi.org/10.1016/j.ejmech.2014.10.047.
  • [8] Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plant extracts containing phenolic compounds. Food Chem. 2006;97(4):654-660. https://doi.org/10.1016/j.foodchem.2005.04.028.
  • [9] Lingadurai S, Nath LK, Kar PK, Besra SE, Joseph RV. Anti-inflammatory and anti-nociceptive activities of methanolic extract of the leaves of Fraxinus floribunda Wallich. Afr J Tradit Complement Altern Med. 2007;4(4):411-416. https://doi.org/10.4314/ajtcam.v4i4.31235.
  • [10] Younis T, Khan MR, Sajid M, Majid M, Zahra Z, Shah NA. Fraxinus xanthoxyloides leaves reduced the level of inflammatory mediators during in vitro and in vivo studies. BMC Complement Altern Med. 2016;16:230. https://doi.org/10.1186/s12906-016-1189-7.
  • [11] Azib L, Debbache-Benaida N, Da Costa G, Atmani-Kilani D, Saidene N, Bouguellid G, Ourabah A, Krisa S, Richard T, Atmani D. Neuroprotective effects of Fraxinus angustifolia Vahl. bark extract against Alzheimer's disease. J Chem Neuroanat. 2020;109:101848. https://doi.org/10.1016/j.jchemneu.2020.101848.
  • [12] Chang HC, Wang SW, Chen CY, Hwang TL, Cheng MJ, Sung PJ, Liao KW, Chen JJ. Secoiridoid glucosides and anti-inflammatory constituents from the stem bark of Fraxinus chinensis. Molecules. 2020;25(24):5911. https://doi.org/10.3390/molecules25245911.
  • [13] Ahmane N, Atmani-Kilani D, Chaher N, Ayouni K, Rahmani-Berboucha M, Da Costa G, Debbache-Benaida N, Richard T, Atmani D. Identification of bioactive compounds from Fraxinus angustifolia extracts with anti-NADH oxidase activity of bovine milk xanthine oxidoreductase. Turk J Biol. 2019;43:133-147.
  • [14] Attanzio A, D'Anneo A, Pappalardo F, Bonina FP, Livrea MA, Allegra M, Tesoriere L. Phenolic composition of hydrophilic extract of manna from Sicilian Fraxinus angustifolia Vahl and its reducing, antioxidant and anti-inflammatory activity in vitro. Antioxidants-Basel. 2019;8(10):494. https://doi.org/10.3390/antiox8100494.
  • [15] Medjahed Z, Atmani-Kilani D, Fauconnier ML, Richard G, Atmani D. Hepatoprotective and antidiabetic activities of Fraxinus angustifolia Vahl extracts in animal models: characterization by high performance liquid chromatography analysis. Turk J Med Sci. 2016;46(3):910-920. https://doi.org/10.3906/sag-1502-13.
  • [16] Subba A, Sahu RK, Bhardwaj S, Mandal P. Alpha glucosidase inhibiting activity and in vivo antidiabetic activity of Fraxinus floribunda bark in streptozotocin-induced diabetic rats. Pharmacogn Res. 2019;11(3):273-278. https://dx.doi.org/10.4103/pr.pr_32_19.
  • [17] Younis T, Jabeen F, Jafri L, Rasul A, Manzoor M, Shaheen M, Riaz A. The protective potential of a Fraxinus xanthoxyloides ethyl acetate fraction against CCl4-induced oxidative stress in the cardiac tissue of rats. RSC Adv. 2020;10(17):10221-10231. https://doi.org/10.1039/c9ra08729j
  • [18] Hadroug A, Belhattab R, Alipieva K, Nedialkov PT. Isofraxisecoside, a new coumarin-secoiridoid from the stem bark of Fraxinus xanthoxyloides. Nat Prod Res. 2019;33(9):1334-1339. https://doi.org/10.1080/14786419.2018.1475379
  • [19] Jeong NH, Yan EJ, Jin M, Lee JY, Choi YA, Park PH, Lee SR, Kim SU, Shin TY, Kwon TK, Jang YH, Song KS, Kim SH. Esculetin from Fraxinus rhynchophylla attenuates atopic skin inflammation by inhibiting the expression of inflammatory cytokines. Int Immunopharmacol. 2018;59:209-216. https://doi.org/10.1016/j.intimp.2018.04.005
  • [20] Lee BZ, Kim KM, Chae S, Jeong SK, Lee S, Hong K, Lee IS. New coumarins isolated from the stem bark of Fraxinus rhynchophylla inhibit human neutrophil elastase and LPS-induced inflammation in RAW 264.7 cells. Phytochem Lett. 2020;35:78-83. https://doi.org/10.4014/jmb.2006.06022
  • [21] Bouguellid G, Russo C, Lavorgna M, Piscitelli C, Ayouni K, Wilson E, Kim HK, Verpoorte R, Choi YH, Kilani-Atmani D, Atmani D, Isidori M. Antimutagenic, antigenotoxic and antiproliferative activities of Fraxinus angustifolia Vahl. leaves and stem bark extracts and their phytochemical composition. PLoS One. 2020;15(4):e0230690. https://doi.org/10.1371/journal.pone.0230690.
  • [22] Sarfraz I, Rasul A, Jabeen F, Younis T, Zahoor MK, Arshad M, Ali M. Fraxinus: A plant with versatile pharmacological and biological activities. Evid Based Complement Alternat Med. 2017;2017:4269868. https://doi.org/10.1155/2017/4269868.
  • [23] Yaltirik F. Fraxinus L. In: Davis PH. (Ed). Flora of Turkey and the Aegean Islands. 6. Edinburgh: University Press, 1978.
  • [24] Klein LC, Zambiasi D, Salgado GR, Delle Monache F, Cechinel V, Buzzi FD. The validation of Calophyllum brasiliense ("guanandi") uses in Brazilian traditional medicine as analgesic by in vivo antinociceptive evaluation and its chemical analysis. Naunyn Schmiedebergs Arch Pharmacol. 2017;390(7):733-739. https://doi.org/10.1007/s00210-017-1366-3.
  • [25] Hunskaar S, Hole K. The formalin test in mice: Dissociation between inflammatory and non-inflammatory pain. Pain 1987;30:103-114. https://doi.org/10.1016/0304-3959(87)90088-1.

Yıl 2023, Cilt: 27 Sayı: 3, 1252 - 1259, 28.06.2025

Yeşim Kaya Yaşar Serhat Sevgi Gülin Renda İrem Çavuşoğlu Nalbantoğlu Mine Duman Feride Sena Sezen

Öz

Kaynakça

  • [1] Caudullo G, Houston Durrant T. Fraxinus angustifolia in Europe: distribution, habitat, usage and threats. In: SanMiguel Ayanz J, de Rigo D, Caudullo G, Houston Durrant T, Mauri A. (Eds). European Atlas of Forest Tree Species. Luxembourg, Publication Office of the European Union, 2016, pp.97.
  • [2] Atmani D, Chaher N, Berboucha M, Ayouni K, Lounis H, Boudaoud H, Debbache N, Atmani D. Antioxidant capacity and phenol content of selected Algerian medicinal plants. Food Chem. 2009;112(2):303-309. https://doi.org/10.1016/j.foodchem.2008.05.077.
  • [3] Hamel T, Sadou S, Seridi R, Boukhdir S, Boulemtafes A. Pratique traditionnelle d’utilisation des plantes médicinales dans la population de la péninsule de l’Edough (nord-est algérien). Ethnopharmacologia 2018;59:65-71.
  • [4] Beloued A. Plantes Médicinales d’Algérie. Algiers. Algeria: Office des Publications Universitaires; 1998.
  • [5] Ayouni K, Berboucha-Rahmani M, Kim HK, Atmani D, Verpoorte R, Choi YH. Metabolomic tool to identify antioxidant compounds of Fraxinus angustifolia leaf and stem bark extracts. Ind Crop Prod. 2016;88:65-77. https://doi.org/10.1016/j.indcrop.2016.01.001.
  • [6] Yazıcıoğlu A, Tuzlacı E. Folk medicinal plants of Trabzon (Turkey). Fitoterapia 1996;67:307-318.
  • [7] Moulaoui K, Caddeo C, Manca ML, Castangia I, Valenti D, Escribano E, Atmani D, Fadda AM, Manconi M. Identification and nanoentrapment of polyphenolic phytocomplex from Fraxinus angustifolia: In vitro and in vivo wound healing potential. Eur J Med Chem. 2015;89:179-188. https://doi.org/10.1016/j.ejmech.2014.10.047.
  • [8] Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plant extracts containing phenolic compounds. Food Chem. 2006;97(4):654-660. https://doi.org/10.1016/j.foodchem.2005.04.028.
  • [9] Lingadurai S, Nath LK, Kar PK, Besra SE, Joseph RV. Anti-inflammatory and anti-nociceptive activities of methanolic extract of the leaves of Fraxinus floribunda Wallich. Afr J Tradit Complement Altern Med. 2007;4(4):411-416. https://doi.org/10.4314/ajtcam.v4i4.31235.
  • [10] Younis T, Khan MR, Sajid M, Majid M, Zahra Z, Shah NA. Fraxinus xanthoxyloides leaves reduced the level of inflammatory mediators during in vitro and in vivo studies. BMC Complement Altern Med. 2016;16:230. https://doi.org/10.1186/s12906-016-1189-7.
  • [11] Azib L, Debbache-Benaida N, Da Costa G, Atmani-Kilani D, Saidene N, Bouguellid G, Ourabah A, Krisa S, Richard T, Atmani D. Neuroprotective effects of Fraxinus angustifolia Vahl. bark extract against Alzheimer's disease. J Chem Neuroanat. 2020;109:101848. https://doi.org/10.1016/j.jchemneu.2020.101848.
  • [12] Chang HC, Wang SW, Chen CY, Hwang TL, Cheng MJ, Sung PJ, Liao KW, Chen JJ. Secoiridoid glucosides and anti-inflammatory constituents from the stem bark of Fraxinus chinensis. Molecules. 2020;25(24):5911. https://doi.org/10.3390/molecules25245911.
  • [13] Ahmane N, Atmani-Kilani D, Chaher N, Ayouni K, Rahmani-Berboucha M, Da Costa G, Debbache-Benaida N, Richard T, Atmani D. Identification of bioactive compounds from Fraxinus angustifolia extracts with anti-NADH oxidase activity of bovine milk xanthine oxidoreductase. Turk J Biol. 2019;43:133-147.
  • [14] Attanzio A, D'Anneo A, Pappalardo F, Bonina FP, Livrea MA, Allegra M, Tesoriere L. Phenolic composition of hydrophilic extract of manna from Sicilian Fraxinus angustifolia Vahl and its reducing, antioxidant and anti-inflammatory activity in vitro. Antioxidants-Basel. 2019;8(10):494. https://doi.org/10.3390/antiox8100494.
  • [15] Medjahed Z, Atmani-Kilani D, Fauconnier ML, Richard G, Atmani D. Hepatoprotective and antidiabetic activities of Fraxinus angustifolia Vahl extracts in animal models: characterization by high performance liquid chromatography analysis. Turk J Med Sci. 2016;46(3):910-920. https://doi.org/10.3906/sag-1502-13.
  • [16] Subba A, Sahu RK, Bhardwaj S, Mandal P. Alpha glucosidase inhibiting activity and in vivo antidiabetic activity of Fraxinus floribunda bark in streptozotocin-induced diabetic rats. Pharmacogn Res. 2019;11(3):273-278. https://dx.doi.org/10.4103/pr.pr_32_19.
  • [17] Younis T, Jabeen F, Jafri L, Rasul A, Manzoor M, Shaheen M, Riaz A. The protective potential of a Fraxinus xanthoxyloides ethyl acetate fraction against CCl4-induced oxidative stress in the cardiac tissue of rats. RSC Adv. 2020;10(17):10221-10231. https://doi.org/10.1039/c9ra08729j
  • [18] Hadroug A, Belhattab R, Alipieva K, Nedialkov PT. Isofraxisecoside, a new coumarin-secoiridoid from the stem bark of Fraxinus xanthoxyloides. Nat Prod Res. 2019;33(9):1334-1339. https://doi.org/10.1080/14786419.2018.1475379
  • [19] Jeong NH, Yan EJ, Jin M, Lee JY, Choi YA, Park PH, Lee SR, Kim SU, Shin TY, Kwon TK, Jang YH, Song KS, Kim SH. Esculetin from Fraxinus rhynchophylla attenuates atopic skin inflammation by inhibiting the expression of inflammatory cytokines. Int Immunopharmacol. 2018;59:209-216. https://doi.org/10.1016/j.intimp.2018.04.005
  • [20] Lee BZ, Kim KM, Chae S, Jeong SK, Lee S, Hong K, Lee IS. New coumarins isolated from the stem bark of Fraxinus rhynchophylla inhibit human neutrophil elastase and LPS-induced inflammation in RAW 264.7 cells. Phytochem Lett. 2020;35:78-83. https://doi.org/10.4014/jmb.2006.06022
  • [21] Bouguellid G, Russo C, Lavorgna M, Piscitelli C, Ayouni K, Wilson E, Kim HK, Verpoorte R, Choi YH, Kilani-Atmani D, Atmani D, Isidori M. Antimutagenic, antigenotoxic and antiproliferative activities of Fraxinus angustifolia Vahl. leaves and stem bark extracts and their phytochemical composition. PLoS One. 2020;15(4):e0230690. https://doi.org/10.1371/journal.pone.0230690.
  • [22] Sarfraz I, Rasul A, Jabeen F, Younis T, Zahoor MK, Arshad M, Ali M. Fraxinus: A plant with versatile pharmacological and biological activities. Evid Based Complement Alternat Med. 2017;2017:4269868. https://doi.org/10.1155/2017/4269868.
  • [23] Yaltirik F. Fraxinus L. In: Davis PH. (Ed). Flora of Turkey and the Aegean Islands. 6. Edinburgh: University Press, 1978.
  • [24] Klein LC, Zambiasi D, Salgado GR, Delle Monache F, Cechinel V, Buzzi FD. The validation of Calophyllum brasiliense ("guanandi") uses in Brazilian traditional medicine as analgesic by in vivo antinociceptive evaluation and its chemical analysis. Naunyn Schmiedebergs Arch Pharmacol. 2017;390(7):733-739. https://doi.org/10.1007/s00210-017-1366-3.
  • [25] Hunskaar S, Hole K. The formalin test in mice: Dissociation between inflammatory and non-inflammatory pain. Pain 1987;30:103-114. https://doi.org/10.1016/0304-3959(87)90088-1.
Toplam 25 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Temel Farmakoloji
Bölüm Articles
Yazarlar

Yeşim Kaya Yaşar 0000-0002-9938-3288

Serhat Sevgi

Gülin Renda 0000-0001-5504-5896

İrem Çavuşoğlu Nalbantoğlu

Mine Duman

Feride Sena Sezen 0000-0002-7379-2518

Yayımlanma Tarihi 28 Haziran 2025
Yayımlandığı Sayı Yıl 2023 Cilt: 27 Sayı: 3

Kaynak Göster

APA Kaya Yaşar, Y., Sevgi, S., Renda, G., Çavuşoğlu Nalbantoğlu, İ., vd. (2025). The in vivo Activity of Fraxinus angustifolia in Pain and Inflammation- Examination of the Biological Activities of the Sub-extracts in Mice. Journal of Research in Pharmacy, 27(3), 1252-1259.
AMA Kaya Yaşar Y, Sevgi S, Renda G, Çavuşoğlu Nalbantoğlu İ, Duman M, Sezen FS. The in vivo Activity of Fraxinus angustifolia in Pain and Inflammation- Examination of the Biological Activities of the Sub-extracts in Mice. J. Res. Pharm. Haziran 2025;27(3):1252-1259.
Chicago Kaya Yaşar, Yeşim, Serhat Sevgi, Gülin Renda, İrem Çavuşoğlu Nalbantoğlu, Mine Duman, ve Feride Sena Sezen. “The in Vivo Activity of Fraxinus Angustifolia in Pain and Inflammation- Examination of the Biological Activities of the Sub-Extracts in Mice”. Journal of Research in Pharmacy 27, sy. 3 (Haziran 2025): 1252-59.
EndNote Kaya Yaşar Y, Sevgi S, Renda G, Çavuşoğlu Nalbantoğlu İ, Duman M, Sezen FS (01 Haziran 2025) The in vivo Activity of Fraxinus angustifolia in Pain and Inflammation- Examination of the Biological Activities of the Sub-extracts in Mice. Journal of Research in Pharmacy 27 3 1252–1259.
IEEE Y. Kaya Yaşar, S. Sevgi, G. Renda, İ. Çavuşoğlu Nalbantoğlu, M. Duman, ve F. S. Sezen, “The in vivo Activity of Fraxinus angustifolia in Pain and Inflammation- Examination of the Biological Activities of the Sub-extracts in Mice”, J. Res. Pharm., c. 27, sy. 3, ss. 1252–1259, 2025.
ISNAD Kaya Yaşar, Yeşim vd. “The in Vivo Activity of Fraxinus Angustifolia in Pain and Inflammation- Examination of the Biological Activities of the Sub-Extracts in Mice”. Journal of Research in Pharmacy 27/3 (Haziran 2025), 1252-1259.
JAMA Kaya Yaşar Y, Sevgi S, Renda G, Çavuşoğlu Nalbantoğlu İ, Duman M, Sezen FS. The in vivo Activity of Fraxinus angustifolia in Pain and Inflammation- Examination of the Biological Activities of the Sub-extracts in Mice. J. Res. Pharm. 2025;27:1252–1259.
MLA Kaya Yaşar, Yeşim vd. “The in Vivo Activity of Fraxinus Angustifolia in Pain and Inflammation- Examination of the Biological Activities of the Sub-Extracts in Mice”. Journal of Research in Pharmacy, c. 27, sy. 3, 2025, ss. 1252-9.
Vancouver Kaya Yaşar Y, Sevgi S, Renda G, Çavuşoğlu Nalbantoğlu İ, Duman M, Sezen FS. The in vivo Activity of Fraxinus angustifolia in Pain and Inflammation- Examination of the Biological Activities of the Sub-extracts in Mice. J. Res. Pharm. 2025;27(3):1252-9.