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Yıl 2021, Cilt: 25 Sayı: 3, 271 - 276, 27.06.2025

Oksana Buchko Viktoriia Havryliak Andriy Pylypets Taras Buchko

https://doi.org/10.29228/jrp.17
Cited By: 1

Öz

Kaynakça

  • [1] Mishra S, Bharagava RN. Toxic and Genotoxic effects of hexavalent chromium in environment and its bioremediation strategies. J Environ Sci Health C Env Carc Ecotoxicol Rev. 2016; 34(1): 1-32. [CrossRef]
  • [2] Mihaylova IV, Smolyagin AI, Krasikov SI, Karaulov AV. Complex assessment of chromium on rats: immunology, biochemistry, trace elements. Immunologiya. 2015; 36(5): 300–305. [article in Russian with an abstract in English]
  • [3] Ray RR. Adverse haematological effects of hexavalent chromium: an overview. Interdiscip Toxicol. 2016; 9(2): 55–65. [CrossRef]
  • [4] Jeevana LM, Srikanth MK, Gopala RA, Anudeep RM. Haematological study in hexavalent chromium toxicity in female wistar rats and its progeny. The Pharma Innov J. 2018; 7(1): 35-38.
  • [5] Stepchenko LM, Shaidek L, Novik V, Sotnikova OP, Haluzina LI. Dosiahnennia ta perspektyvy zastosuvannia huminovykh rechovyn u silskomu hospodarstvi. Dnipro: Astra-Prynt; 2017.
  • [6] Nurten G, Umit P, Hakan B. Effects of supplemental humic acid on ruminal fermentation and blood variables in rams. Ital J Anim Sci. 2010; 9: 390-393. [CrossRef]
  • [7] Wang Q, Ying J, Zou P, Zhou Y, Wang B, Yu D, Li W, Zhan X. Effects of Dietary Supplementation of Humic Acid Sodium and Zinc Oxide on Growth Performance, Immune Status and Antioxidant Capacity of Weaned Piglets.Animals. 2020; 10(11): 2104. [CrossRef]
  • [8] Balakrishnan R, Satish Kumar CS, Rani MU, Srikanth MK, Boobalan G, Reddy AG. An evaluation of the protective role of α-tocopherol on free radical induced hepatotoxicity and nephrotoxicity due to chromium in rats. Indian J Pharmacol. 2013; 45: 490-495. [CrossRef]
  • [9] Lebedeva EI, Myadelets OD, Kichigina TN, Grushin VN. Influence of toxic liver cyrosis on the content of general protein and protein fractions in serum of rat blood. J New Med Tech, eEdition. 2018; 2: 144-150. [CrossRef]
  • [10] Reham AA, Mounes HAM, Ahmed KM. Use of humic acid and Yucca extract as a benefactor on water quality and their impact on some hematological and histological parameters of Oreochromis niloticus. Egypt J Aquatic Biol Fish. 2018; 22(5): 447-460.
  • [11] Verigo NS, Ryzhkovaskaya EL, Kuznetsova TE, Ulashchik VS. Izmenenie strukturno-funkczionalnogo sostoyaniya pecheni u krys s gepatitom pod vliyaniem malomineralizovannoj mineralnoj vody s povyshennym soderzhaniem guminovykh kislot. Russian Journal of Physiotherapy, Balneology and Rehabilitation. 2013; 12(5): 4-10.
  • [12] Shil K, Pal S. Metabolic adaptability in hexavalent chromium-treated renal tissue: an in vivo study. Clin Kidney J. 2018; 11(2): 222–229. [CrossRef]
  • [13] Hegazy R, Salama A, Mansour D, Hassan A. Renoprotective effect of lactoferrin against chromium-induced acute kidney injury in rats: Involvement of IL-18 and IGF-1 Inhibition. PLoS ONE. 2016; 11(3): 1-18. [CrossRef]
  • [14] Burmas NI. The state of antioxidant system and bile formation function in the organism of rats affected by hexavalent chromium compounds. Med Clin Chem. 2016; 18(1): 89-93. [article in Ukranian with an abstract in English] [CrossRef]
  • [15] Buchko O, Havryliak V. Effect of the Supplement of Humic Origin on the Free Radical Processes and Histological Changes in the Tissues of Rats Affected by Chromium (VI). Biointerface Res Appl Chem. 2021; 11(3): 10996 – 11008. [CrossRef]
  • [16] Sahin A, Iskender H, Terim Kapakin KA, Altinkaynak K, Hayirli A, Gonultas A, Kaynar O. The effect of humic acid substances on the thyroid function and structure in lead poisoning. Braz J Poultry Sci. 2016; 18(4): 649-654. [CrossRef]
  • [17] Szabó J, Vucskits AV, Berta E, Andrásofszky E, Bersényi A, Hullár I. Effect of fulvic and humic acids on iron and manganese homeostasis in rats. Acta Vet Hung. 2017; 65(1): 66–80. [CrossRef]
  • [18] Lavrik NL, Ilyitcheva ТN. Study of the Influence of Humic Acid Macromolecules on the Structure of Erythrocytes of Some Animals by the Method of Absorption. Bioph Chem. 2019; 1-12. [CrossRef]
  • [19] Vlizlo VV (Ed.). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni. Lviv: Spolom;2012.
  • [20] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259): 680-685. [CrossRef]

Effect of food supplement of humic origin on the hematological and biochemical parameters in the Cr (VI) exposed rats

Yıl 2021, Cilt: 25 Sayı: 3, 271 - 276, 27.06.2025

Oksana Buchko Viktoriia Havryliak Andriy Pylypets Taras Buchko

https://doi.org/10.29228/jrp.17
Cited By: 1

Öz

The study aimed to investigate the effect of Humilid, a biologically active food supplement, on biochemical and hematologic parameters in the blood of rats exposed to hexavalent Chromium. Wistar male rats were used in experiments. All animals were divided into 4 groups. Animals of experimental groups D2 and D3 received a 1% solution of food supplement Humilid in a dose of 2 ml/kg of body weight during 28 days. Rats of groups D1 and D2 were injected with potassium bichromate (K2Cr2O7) in the dose of 2 mg Cr (VI)/kg of body weight from 14 days of the experiment. Animals of the C group were administered a 0.9% NaCl solution for 14 days. Data were analyzed using the ANOVA test. It has been found that administration of potassium bichromate caused the cytotoxic effect on rat’s organism, inhibition of the synthetic function of the liver, hypoglycemic effect, toxic anemia and leukocytosis. The treatment with humic substances provided a marked normalization of the biochemical and hematological parameters in the blood of chromium-exposed animals. Biologic active supplement Humilid reduces the toxic effect of chromium on the rat body, normalizes metabolism, and exhibits hepatoprotective and adaptogenic effects. Our findings show that Humilid supplementation may be helpful in the abrogation of heavy metal toxicity.

Anahtar Kelimeler

Humilid Chromium rats hematology biochemical parameters.

Kaynakça

  • [1] Mishra S, Bharagava RN. Toxic and Genotoxic effects of hexavalent chromium in environment and its bioremediation strategies. J Environ Sci Health C Env Carc Ecotoxicol Rev. 2016; 34(1): 1-32. [CrossRef]
  • [2] Mihaylova IV, Smolyagin AI, Krasikov SI, Karaulov AV. Complex assessment of chromium on rats: immunology, biochemistry, trace elements. Immunologiya. 2015; 36(5): 300–305. [article in Russian with an abstract in English]
  • [3] Ray RR. Adverse haematological effects of hexavalent chromium: an overview. Interdiscip Toxicol. 2016; 9(2): 55–65. [CrossRef]
  • [4] Jeevana LM, Srikanth MK, Gopala RA, Anudeep RM. Haematological study in hexavalent chromium toxicity in female wistar rats and its progeny. The Pharma Innov J. 2018; 7(1): 35-38.
  • [5] Stepchenko LM, Shaidek L, Novik V, Sotnikova OP, Haluzina LI. Dosiahnennia ta perspektyvy zastosuvannia huminovykh rechovyn u silskomu hospodarstvi. Dnipro: Astra-Prynt; 2017.
  • [6] Nurten G, Umit P, Hakan B. Effects of supplemental humic acid on ruminal fermentation and blood variables in rams. Ital J Anim Sci. 2010; 9: 390-393. [CrossRef]
  • [7] Wang Q, Ying J, Zou P, Zhou Y, Wang B, Yu D, Li W, Zhan X. Effects of Dietary Supplementation of Humic Acid Sodium and Zinc Oxide on Growth Performance, Immune Status and Antioxidant Capacity of Weaned Piglets.Animals. 2020; 10(11): 2104. [CrossRef]
  • [8] Balakrishnan R, Satish Kumar CS, Rani MU, Srikanth MK, Boobalan G, Reddy AG. An evaluation of the protective role of α-tocopherol on free radical induced hepatotoxicity and nephrotoxicity due to chromium in rats. Indian J Pharmacol. 2013; 45: 490-495. [CrossRef]
  • [9] Lebedeva EI, Myadelets OD, Kichigina TN, Grushin VN. Influence of toxic liver cyrosis on the content of general protein and protein fractions in serum of rat blood. J New Med Tech, eEdition. 2018; 2: 144-150. [CrossRef]
  • [10] Reham AA, Mounes HAM, Ahmed KM. Use of humic acid and Yucca extract as a benefactor on water quality and their impact on some hematological and histological parameters of Oreochromis niloticus. Egypt J Aquatic Biol Fish. 2018; 22(5): 447-460.
  • [11] Verigo NS, Ryzhkovaskaya EL, Kuznetsova TE, Ulashchik VS. Izmenenie strukturno-funkczionalnogo sostoyaniya pecheni u krys s gepatitom pod vliyaniem malomineralizovannoj mineralnoj vody s povyshennym soderzhaniem guminovykh kislot. Russian Journal of Physiotherapy, Balneology and Rehabilitation. 2013; 12(5): 4-10.
  • [12] Shil K, Pal S. Metabolic adaptability in hexavalent chromium-treated renal tissue: an in vivo study. Clin Kidney J. 2018; 11(2): 222–229. [CrossRef]
  • [13] Hegazy R, Salama A, Mansour D, Hassan A. Renoprotective effect of lactoferrin against chromium-induced acute kidney injury in rats: Involvement of IL-18 and IGF-1 Inhibition. PLoS ONE. 2016; 11(3): 1-18. [CrossRef]
  • [14] Burmas NI. The state of antioxidant system and bile formation function in the organism of rats affected by hexavalent chromium compounds. Med Clin Chem. 2016; 18(1): 89-93. [article in Ukranian with an abstract in English] [CrossRef]
  • [15] Buchko O, Havryliak V. Effect of the Supplement of Humic Origin on the Free Radical Processes and Histological Changes in the Tissues of Rats Affected by Chromium (VI). Biointerface Res Appl Chem. 2021; 11(3): 10996 – 11008. [CrossRef]
  • [16] Sahin A, Iskender H, Terim Kapakin KA, Altinkaynak K, Hayirli A, Gonultas A, Kaynar O. The effect of humic acid substances on the thyroid function and structure in lead poisoning. Braz J Poultry Sci. 2016; 18(4): 649-654. [CrossRef]
  • [17] Szabó J, Vucskits AV, Berta E, Andrásofszky E, Bersényi A, Hullár I. Effect of fulvic and humic acids on iron and manganese homeostasis in rats. Acta Vet Hung. 2017; 65(1): 66–80. [CrossRef]
  • [18] Lavrik NL, Ilyitcheva ТN. Study of the Influence of Humic Acid Macromolecules on the Structure of Erythrocytes of Some Animals by the Method of Absorption. Bioph Chem. 2019; 1-12. [CrossRef]
  • [19] Vlizlo VV (Ed.). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni. Lviv: Spolom;2012.
  • [20] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259): 680-685. [CrossRef]
Toplam 20 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Eczacılık ve İlaç Bilimleri (Diğer)
Bölüm Articles
Yazarlar

Oksana Buchko

Viktoriia Havryliak

Andriy Pylypets

Taras Buchko

Yayımlanma Tarihi 27 Haziran 2025
Yayımlandığı Sayı Yıl 2021 Cilt: 25 Sayı: 3

Kaynak Göster

APA Buchko, O., Havryliak, V., Pylypets, A., Buchko, T. (2025). Effect of food supplement of humic origin on the hematological and biochemical parameters in the Cr (VI) exposed rats. Journal of Research in Pharmacy, 25(3), 271-276. https://doi.org/10.29228/jrp.17
AMA Buchko O, Havryliak V, Pylypets A, Buchko T. Effect of food supplement of humic origin on the hematological and biochemical parameters in the Cr (VI) exposed rats. J. Res. Pharm. Haziran 2025;25(3):271-276. doi:10.29228/jrp.17
Chicago Buchko, Oksana, Viktoriia Havryliak, Andriy Pylypets, ve Taras Buchko. “Effect of Food Supplement of Humic Origin on the Hematological and Biochemical Parameters in the Cr (VI) Exposed Rats”. Journal of Research in Pharmacy 25, sy. 3 (Haziran 2025): 271-76. https://doi.org/10.29228/jrp.17.
EndNote Buchko O, Havryliak V, Pylypets A, Buchko T (01 Haziran 2025) Effect of food supplement of humic origin on the hematological and biochemical parameters in the Cr (VI) exposed rats. Journal of Research in Pharmacy 25 3 271–276.
IEEE O. Buchko, V. Havryliak, A. Pylypets, ve T. Buchko, “Effect of food supplement of humic origin on the hematological and biochemical parameters in the Cr (VI) exposed rats”, J. Res. Pharm., c. 25, sy. 3, ss. 271–276, 2025, doi: 10.29228/jrp.17.
ISNAD Buchko, Oksana vd. “Effect of Food Supplement of Humic Origin on the Hematological and Biochemical Parameters in the Cr (VI) Exposed Rats”. Journal of Research in Pharmacy 25/3 (Haziran 2025), 271-276. https://doi.org/10.29228/jrp.17.
JAMA Buchko O, Havryliak V, Pylypets A, Buchko T. Effect of food supplement of humic origin on the hematological and biochemical parameters in the Cr (VI) exposed rats. J. Res. Pharm. 2025;25:271–276.
MLA Buchko, Oksana vd. “Effect of Food Supplement of Humic Origin on the Hematological and Biochemical Parameters in the Cr (VI) Exposed Rats”. Journal of Research in Pharmacy, c. 25, sy. 3, 2025, ss. 271-6, doi:10.29228/jrp.17.
Vancouver Buchko O, Havryliak V, Pylypets A, Buchko T. Effect of food supplement of humic origin on the hematological and biochemical parameters in the Cr (VI) exposed rats. J. Res. Pharm. 2025;25(3):271-6.

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Studia Biologica
https://doi.org/10.30970/sbi.1701.701