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Yıl 2021, Cilt: 25 Sayı: 4, 398 - 406, 27.06.2025

Gülşah Erel Akbaba , Hasan Akbaba

https://doi.org/10.29228/jrp.30
Cited By: 1

Öz

Kaynakça

  • [1] Ni R, Zhou J, Hossain N, Chau Y. Virus-inspired nucleic acid delivery system: Linking virus and viral mimicry. Adv Drug Deliv Rev. 2016; 106: 3–26. [CrossRef]
  • [2] Salva E, Turan SÖ, Eren F, Akbuğa J. The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGF. Int J Pharm. 2014; 478(1): 147–154. [CrossRef]
  • [3] Erel-Akbaba G, Akbaba H, Kantarcı AG. Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment. Marmara Pharm J. 2018 Apr 6; 22(2): 299–306. [CrossRef]
  • [4] Foldvari M, Chen DW, Nafissi N, Calderon D, Narsineni L, Rafiee A. Non-viral gene therapy: Gains and challenges of non-invasive administration methods. J Control Release. 2016; 240: 165–190. [CrossRef]
  • [5] Shin MD, Shukla S, Chung YH, Beiss V, Chan SK, Ortega-Rivera OA, Wirth DM, Chen A, Sack M, Pokorski JK, Steinmetz NF. COVID-19 vaccine development and a potential nanomaterial path forward. Nat Nanotechnol. 2020;15(8): 646–655. [CrossRef]
  • [6] Erel-Akbaba G, Carvalho LA, Tian T, Zinter M, Akbaba H, Obeid PJ, Chiocca EA, Weissleder R, Kantarci AG, Tannous BA. Radiation-Induced Targeted Nanoparticle-Based Gene Delivery for Brain Tumor Therapy. ACS Nano. 2019; 13(4): 4028–4040. [CrossRef]
  • [7] Rassu G, Soddu E, Posadino AM, Pintus G, Sarmento B, Giunchedi P, Gavini E. Nose-to-brain delivery of BACE1 siRNA loaded in solid lipid nanoparticles for Alzheimer’s therapy. Colloids Surfaces B Biointerfaces. 2017; 152: 296–301. [CrossRef]
  • [8] Yang S, Zheng Y, Chen J, Zhang Q, Zhao D, Han D, Chen X. Comprehensive study of cationic liposomes composed of DC-Chol and cholesterol with different mole ratios for gene transfection. Colloids Surfaces B Biointerfaces. 2013;101: 6–13. [CrossRef]
  • [9] Elsana H, Olusanya TOB, Carr-wilkinson J, Darby S, Faheem A, Elkordy AA. Evaluation of novel cationic gene based liposomes with cyclodextrin prepared by thin film hydration and microfluidic systems. Sci Rep. 2019; 9(1): 1–17. [CrossRef]
  • [10] Ozpolat B, Sood AK, Lopez-Berestein G. Liposomal siRNA nanocarriers for cancer therapy. Adv Drug Deliv Rev. 2014; 66: 110–116. [CrossRef]
  • [11] Zhuang Y, Ma Y, Wang C, Hai L, Yan C, Zhang Y, Liu F, Cai L. PEGylated cationic liposomes robustly augment vaccine-induced immune responses: Role of lymphatic trafficking and biodistribution. J Control Release. 2012; 159(1):135–142. [CrossRef]
  • [12] Akula S, Gurram AK, Devireddy SR, Deshpande PB. Evaluation of Surfactant Effect on Self Micro Emulsifying Drug Delivery System (SMEDDS) of Lercanidipine Hydrochloride: Formulation and Evaluation. J Pharm Innov. 2015;10(4): 374–387. [CrossRef]
  • [13] Çelik Soysal A, Şahbaz S, Uğurlu T, Sezer AD. Preparation and and characterization of poly(lactic-co-glycolic acid) nanoparticles containing TGF- β1 and evaluation of in vitro wound healing effect. J Res Pharm. 2020; 24(2): 277–289. [CrossRef]
  • [14] Akbaba H, Erel Akbaba G, Kantarcı AG. Development and evaluation of antisense shRNA-encoding plasmid loaded solid lipid nanoparticles against 5-α reductase activity. J Drug Deliv Sci Technol. 2018; 44: 270–277. [CrossRef]
  • [15] Deng Y, Wang CC, Choy KW, Du Q, Chen J, Wang Q, Li L, Chung TKH, Tang T. Therapeutic potentials of gene silencing by RNA interference: Principles, challenges, and new strategies. Gene. 2014; 538(2): 217–227. [CrossRef]
  • [16] Şenel B. In vitro preliminary studies of chitooligosaccharide coated nanostructured lipidic nanoparticles for efficient gene delivery. J Res Pharm. 2019; 23(4): 671–681. [CrossRef]
  • [17] de Jesus MB, Zuhorn IS. Solid lipid nanoparticles as nucleic acid delivery system: Properties and molecular mechanisms. J Control Release. 2015; 201:1–13. [CrossRef]
  • [18] dos Santos Rodrigues B, Oue H, Banerjee A, Kanekiyo T, Singh J. Dual functionalized liposome-mediated gene delivery across triple co-culture blood brain barrier model and specific in vivo neuronal transfection. J Control Release. 2018; 286: 264–278. [CrossRef]
  • [19] Jeong UH, Garripelli VK, Jo S, Myung CS, Hwang SJ, Kim JK, Park JS. Potential of pH-sensitive polymer-anchored cationic liposomes for combinatorial anticancer therapy with doxorubicin and siRNA. J Drug Deliv Sci Technol. 2014;24(1): 27–32. [CrossRef]
  • [20] Capan Y, Woo BH, Gebrekidan S, Ahmed S, DeLuca PP. Stability of poly(L-lysine)-complexed plasmid DNA during mechanical stress and DNase I treatment. Pharm Dev Technol. 1999; 4(4): 491–498. [CrossRef]
  • [21] Liu Y, An S, Li J, Kuang Y, He X, Guo Y, Ma H, Zhang Y, Ji B, Jiang C. Brain-targeted co-delivery of therapeutic gene and peptide by multifunctional nanoparticles in Alzheimer’s disease mice. Biomaterials . 2016; 80: 33–45. [CrossRef]
  • [22] Mozafari MR, Reed CJ, Rostron C. Cytotoxicity evaluation of anionic nanoliposomes and nanolipoplexes prepared by the heating method without employing volatile solvents and detergents. Pharmazie. 2007; 62(3): 205–209. [CrossRef]
  • [23] Knudsen KB, Northeved H, Pramod Kumar EK, Permin A, Gjetting T, Andresen TL, et al. In vivo toxicity of cationic micelles and liposomes. Nanomedicine Nanotechnology, Biol Med. 2015; 11(2): 467–477. [CrossRef]
  • [24] Siafaka PI, Okur NÜ, Karantas ID, Okur ME, Gündoğdu EA. Current update on nanoplatforms as therapeutic and diagnostic tools: A review for the materials used as nanotheranostics and imaging modalities. Asian J Pharm Sci. 2021; 16(1): 24–46.[CrossRef]
  • [25] Adami RC, Collard WT, Gupta SA, KwoK KY, Bonadio J, Rice KG. Stability of Peptide-Condensed Plasmid DNA Formulations. J Pharm Sci. 1998; 87(6): 678–683. [CrossRef]
  • [26] Ozder M, Akbaba H. Optimization and screening of solid lipid nanoparticle production for gene delivery by factorial design and response surface methodology. Exp Biomed Res. 2021; 4(1): 23–37. [CrossRef]
  • [27] İsar S, Akbaba H, Erel-Akbaba G, Başpınar Y. Development and characterization of cationic nanoemulsions as non-viral vectors for plasmid DNA delivery. J Res Pharm. 2020; 24(6): 952–960. [CrossRef]
  • [28] Wang MO, Etheridge JM, Thompson JA, Vorwald CE, Dean D, Fisher JP. Evaluation of the in vitro cytotoxicity of cross-linked biomaterials. Biomacromolecules. 2013; 14(5): 1321–1329. [CrossRef]
  • [29] Li M, Han M, Sun Y, Hua Y, Chen G, Zhang L. Oligoarginine mediated collagen/chitosan gel composite for cutaneous wound healing. Int J Biol Macromol. 2019; 122: 1120–1127.[CrossRef]

A comparative study of cationic liposomes for gene delivery

Yıl 2021, Cilt: 25 Sayı: 4, 398 - 406, 27.06.2025

Gülşah Erel Akbaba , Hasan Akbaba

https://doi.org/10.29228/jrp.30
Cited By: 1

Öz

In the field of gene delivery, non-viral vectors have become more attractive carriers for nucleic acids since they can overcome the significant drawbacks of viral systems such as safety, immunogenicity, and oncogenicity. Among non-viral vectors, cationic liposome-mediated gene delivery gives promising results for gene therapy approaches. This study aimed to develop cationic liposomes and examine the effectiveness in terms of gene delivery. For this purpose, cholesterol, lecithin, and cationic lipid containing liposomes have been developed by film hydration method. Two different cationic lipids, DDAB and EQ, and their different mole ratios were investigated. Characterization studies showed that obtained liposomes have appropriate physiochemical characteristics (~100 nm, homogenous in size and positive zeta potential) for gene delivery. Gel retardation assay revealed that they have DNA binding and protection ability against nucleases. According to the cytotoxicity evaluation performed on L929 cell line, EQ containing liposomes shows significantly less toxicity comparing DDAB containing liposomes (p<0.05). Furthermore, in vitro transfection study revealed that increasing the EQ mole ratio has increased transfection ability. The stability results showed that the optimal liposome which contains EQ in a higher mole ratio is stable during 90 days at 4oC. Based on these findings, we propose that the developed optimal liposome system in this study could be considered as a suitable nucleic acid delivery vehicle base for gene therapy.

Anahtar Kelimeler

Liposome gene delivery transfection cytotoxicity DDAB esterquate.

Kaynakça

  • [1] Ni R, Zhou J, Hossain N, Chau Y. Virus-inspired nucleic acid delivery system: Linking virus and viral mimicry. Adv Drug Deliv Rev. 2016; 106: 3–26. [CrossRef]
  • [2] Salva E, Turan SÖ, Eren F, Akbuğa J. The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGF. Int J Pharm. 2014; 478(1): 147–154. [CrossRef]
  • [3] Erel-Akbaba G, Akbaba H, Kantarcı AG. Development and in vitro evaluation of positive-charged solid lipid nanoparticles as nucleic acid delivery system in glioblastoma treatment. Marmara Pharm J. 2018 Apr 6; 22(2): 299–306. [CrossRef]
  • [4] Foldvari M, Chen DW, Nafissi N, Calderon D, Narsineni L, Rafiee A. Non-viral gene therapy: Gains and challenges of non-invasive administration methods. J Control Release. 2016; 240: 165–190. [CrossRef]
  • [5] Shin MD, Shukla S, Chung YH, Beiss V, Chan SK, Ortega-Rivera OA, Wirth DM, Chen A, Sack M, Pokorski JK, Steinmetz NF. COVID-19 vaccine development and a potential nanomaterial path forward. Nat Nanotechnol. 2020;15(8): 646–655. [CrossRef]
  • [6] Erel-Akbaba G, Carvalho LA, Tian T, Zinter M, Akbaba H, Obeid PJ, Chiocca EA, Weissleder R, Kantarci AG, Tannous BA. Radiation-Induced Targeted Nanoparticle-Based Gene Delivery for Brain Tumor Therapy. ACS Nano. 2019; 13(4): 4028–4040. [CrossRef]
  • [7] Rassu G, Soddu E, Posadino AM, Pintus G, Sarmento B, Giunchedi P, Gavini E. Nose-to-brain delivery of BACE1 siRNA loaded in solid lipid nanoparticles for Alzheimer’s therapy. Colloids Surfaces B Biointerfaces. 2017; 152: 296–301. [CrossRef]
  • [8] Yang S, Zheng Y, Chen J, Zhang Q, Zhao D, Han D, Chen X. Comprehensive study of cationic liposomes composed of DC-Chol and cholesterol with different mole ratios for gene transfection. Colloids Surfaces B Biointerfaces. 2013;101: 6–13. [CrossRef]
  • [9] Elsana H, Olusanya TOB, Carr-wilkinson J, Darby S, Faheem A, Elkordy AA. Evaluation of novel cationic gene based liposomes with cyclodextrin prepared by thin film hydration and microfluidic systems. Sci Rep. 2019; 9(1): 1–17. [CrossRef]
  • [10] Ozpolat B, Sood AK, Lopez-Berestein G. Liposomal siRNA nanocarriers for cancer therapy. Adv Drug Deliv Rev. 2014; 66: 110–116. [CrossRef]
  • [11] Zhuang Y, Ma Y, Wang C, Hai L, Yan C, Zhang Y, Liu F, Cai L. PEGylated cationic liposomes robustly augment vaccine-induced immune responses: Role of lymphatic trafficking and biodistribution. J Control Release. 2012; 159(1):135–142. [CrossRef]
  • [12] Akula S, Gurram AK, Devireddy SR, Deshpande PB. Evaluation of Surfactant Effect on Self Micro Emulsifying Drug Delivery System (SMEDDS) of Lercanidipine Hydrochloride: Formulation and Evaluation. J Pharm Innov. 2015;10(4): 374–387. [CrossRef]
  • [13] Çelik Soysal A, Şahbaz S, Uğurlu T, Sezer AD. Preparation and and characterization of poly(lactic-co-glycolic acid) nanoparticles containing TGF- β1 and evaluation of in vitro wound healing effect. J Res Pharm. 2020; 24(2): 277–289. [CrossRef]
  • [14] Akbaba H, Erel Akbaba G, Kantarcı AG. Development and evaluation of antisense shRNA-encoding plasmid loaded solid lipid nanoparticles against 5-α reductase activity. J Drug Deliv Sci Technol. 2018; 44: 270–277. [CrossRef]
  • [15] Deng Y, Wang CC, Choy KW, Du Q, Chen J, Wang Q, Li L, Chung TKH, Tang T. Therapeutic potentials of gene silencing by RNA interference: Principles, challenges, and new strategies. Gene. 2014; 538(2): 217–227. [CrossRef]
  • [16] Şenel B. In vitro preliminary studies of chitooligosaccharide coated nanostructured lipidic nanoparticles for efficient gene delivery. J Res Pharm. 2019; 23(4): 671–681. [CrossRef]
  • [17] de Jesus MB, Zuhorn IS. Solid lipid nanoparticles as nucleic acid delivery system: Properties and molecular mechanisms. J Control Release. 2015; 201:1–13. [CrossRef]
  • [18] dos Santos Rodrigues B, Oue H, Banerjee A, Kanekiyo T, Singh J. Dual functionalized liposome-mediated gene delivery across triple co-culture blood brain barrier model and specific in vivo neuronal transfection. J Control Release. 2018; 286: 264–278. [CrossRef]
  • [19] Jeong UH, Garripelli VK, Jo S, Myung CS, Hwang SJ, Kim JK, Park JS. Potential of pH-sensitive polymer-anchored cationic liposomes for combinatorial anticancer therapy with doxorubicin and siRNA. J Drug Deliv Sci Technol. 2014;24(1): 27–32. [CrossRef]
  • [20] Capan Y, Woo BH, Gebrekidan S, Ahmed S, DeLuca PP. Stability of poly(L-lysine)-complexed plasmid DNA during mechanical stress and DNase I treatment. Pharm Dev Technol. 1999; 4(4): 491–498. [CrossRef]
  • [21] Liu Y, An S, Li J, Kuang Y, He X, Guo Y, Ma H, Zhang Y, Ji B, Jiang C. Brain-targeted co-delivery of therapeutic gene and peptide by multifunctional nanoparticles in Alzheimer’s disease mice. Biomaterials . 2016; 80: 33–45. [CrossRef]
  • [22] Mozafari MR, Reed CJ, Rostron C. Cytotoxicity evaluation of anionic nanoliposomes and nanolipoplexes prepared by the heating method without employing volatile solvents and detergents. Pharmazie. 2007; 62(3): 205–209. [CrossRef]
  • [23] Knudsen KB, Northeved H, Pramod Kumar EK, Permin A, Gjetting T, Andresen TL, et al. In vivo toxicity of cationic micelles and liposomes. Nanomedicine Nanotechnology, Biol Med. 2015; 11(2): 467–477. [CrossRef]
  • [24] Siafaka PI, Okur NÜ, Karantas ID, Okur ME, Gündoğdu EA. Current update on nanoplatforms as therapeutic and diagnostic tools: A review for the materials used as nanotheranostics and imaging modalities. Asian J Pharm Sci. 2021; 16(1): 24–46.[CrossRef]
  • [25] Adami RC, Collard WT, Gupta SA, KwoK KY, Bonadio J, Rice KG. Stability of Peptide-Condensed Plasmid DNA Formulations. J Pharm Sci. 1998; 87(6): 678–683. [CrossRef]
  • [26] Ozder M, Akbaba H. Optimization and screening of solid lipid nanoparticle production for gene delivery by factorial design and response surface methodology. Exp Biomed Res. 2021; 4(1): 23–37. [CrossRef]
  • [27] İsar S, Akbaba H, Erel-Akbaba G, Başpınar Y. Development and characterization of cationic nanoemulsions as non-viral vectors for plasmid DNA delivery. J Res Pharm. 2020; 24(6): 952–960. [CrossRef]
  • [28] Wang MO, Etheridge JM, Thompson JA, Vorwald CE, Dean D, Fisher JP. Evaluation of the in vitro cytotoxicity of cross-linked biomaterials. Biomacromolecules. 2013; 14(5): 1321–1329. [CrossRef]
  • [29] Li M, Han M, Sun Y, Hua Y, Chen G, Zhang L. Oligoarginine mediated collagen/chitosan gel composite for cutaneous wound healing. Int J Biol Macromol. 2019; 122: 1120–1127.[CrossRef]
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Farmasotik Biyoteknoloji, İlaç Dağıtım Teknolojileri
Bölüm Articles
Yazarlar

Gülşah Erel Akbaba

Hasan Akbaba

Yayımlanma Tarihi 27 Haziran 2025
Yayımlandığı Sayı Yıl 2021 Cilt: 25 Sayı: 4

Kaynak Göster

APA Erel Akbaba, G., & Akbaba, H. (2025). A comparative study of cationic liposomes for gene delivery. Journal of Research in Pharmacy, 25(4), 398-406. https://doi.org/10.29228/jrp.30
AMA Erel Akbaba G, Akbaba H. A comparative study of cationic liposomes for gene delivery. J. Res. Pharm. Haziran 2025;25(4):398-406. doi:10.29228/jrp.30
Chicago Erel Akbaba, Gülşah, ve Hasan Akbaba. “A Comparative Study of Cationic Liposomes for Gene Delivery”. Journal of Research in Pharmacy 25, sy. 4 (Haziran 2025): 398-406. https://doi.org/10.29228/jrp.30.
EndNote Erel Akbaba G, Akbaba H (01 Haziran 2025) A comparative study of cationic liposomes for gene delivery. Journal of Research in Pharmacy 25 4 398–406.
IEEE G. Erel Akbaba ve H. Akbaba, “A comparative study of cationic liposomes for gene delivery”, J. Res. Pharm., c. 25, sy. 4, ss. 398–406, 2025, doi: 10.29228/jrp.30.
ISNAD Erel Akbaba, Gülşah - Akbaba, Hasan. “A Comparative Study of Cationic Liposomes for Gene Delivery”. Journal of Research in Pharmacy 25/4 (Haziran 2025), 398-406. https://doi.org/10.29228/jrp.30.
JAMA Erel Akbaba G, Akbaba H. A comparative study of cationic liposomes for gene delivery. J. Res. Pharm. 2025;25:398–406.
MLA Erel Akbaba, Gülşah ve Hasan Akbaba. “A Comparative Study of Cationic Liposomes for Gene Delivery”. Journal of Research in Pharmacy, c. 25, sy. 4, 2025, ss. 398-06, doi:10.29228/jrp.30.
Vancouver Erel Akbaba G, Akbaba H. A comparative study of cationic liposomes for gene delivery. J. Res. Pharm. 2025;25(4):398-406.

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