Araştırma Makalesi
BibTex RIS Kaynak Göster

A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries

Yıl 2023, Cilt: 1 Sayı: 2, 55 - 63, 30.04.2023

Umut Kökbaş

https://doi.org/10.58241/ksbd.6

Öz

Abstract
Statement of Problem: Many mechanisms in the formation of dental caries, but the common point of these mechanisms is the proliferation of microorganisms in the mouth and mostly on the tooth surface. Objective: Although there are approximately 200 to 300 different microorganisms in human dental plaque, Neisseria spp. and S. mutans. The common point of these microorganisms is that they produce lactic acid. In this study, it is aimed to use a biosensor, which is a bioelectrochemical measurement system that combines the measurement capability of physical analysis systems and the specificity of biochemical systems.Material-Method: In this study, lactate oxidase enzyme was immobilised on the gold working electrode surface by means of bovine serum albumin and gelatine. The enzyme was arrested on the electrode surface with the aid of the cross-linking agent glutaraldehyde. Cyclic voltammetry current scanning was performed in lactic acid biosensor preliminary studies. Optimisation studies were conducted in the current range obtained from the peak in the cyclic voltammogram. Within the scope of the optimisation studies, optimum operating temperature and optimum operating pH of the biosensor were tested.Results: An anodic peak at +0.2 V potential was obtained in the cyclic voltammogram drawn according to the reaction on the working electrode surface. In the optimisation studies conducted, it was found that the optimum operating conditions of the biosensor were at 35 °C and pH 6.5.Conclusion: According to the data obtained from the pre-study of the bioelectrochemical method, the concentration of lactic acid in the solution can be determined. With the completion of further studies, a portable lactic acid biosensor can be made. As a result, as a result of the preliminary study, the lactic acid biosensors designed to determine in vitro lactic acid for the early diagnosis of dental caries in the future is promising.

Anahtar Kelimeler

Biosensor Dental Caries Lactic Acid Lactate Oxidase

Kaynakça

  • Assiri, S. A., El Meligy, O., Alzain, I. O., & Bamashmous, N. O. (2022). Assessment of dental caries and salivary characteristics among type 1 diabetic Saudi children. J Dent Sci,17(4), 1634-1639. https://doi.org/10.1016/j.jds.2022.03.010
  • Benn, A. M. L., Heng, N. C. K., Thomson, W. M., & Broadbent, J. M. (2022). Plaque and dental caries risk in mid-life. Caries Res. https://doi.org/10.1159/000527255
  • Borg-Bartolo, R., Roccuzzo, A., Molinero-Mourelle, P., Schimmel, M., Gambetta-Tessini, K., Chaurasia, A., Koca-Unsal, R. B., Tennert, C., Giacaman, R., & Campus, G. (2022). Global prevalence of edentulism and dental caries in middle-aged and elderly persons: A systematic review and meta-analysis. J Dent,127,104335. https://doi.org/10.1016/j.jdent.2022.104335
  • Chaudhry, S., Haroon, F., Irfan Waheed, K. A., Victor, G., Shahzad, M., & Fatima, B. (2022). Blood Lactate Levels And Lactate Clearance As Predictors Of Mortality In Neonatal Sepsis. J Ayub Med Coll Abbottabad,34(3), 438-441. https://doi.org/10.55519/JAMC-03-9087
  • Fernandez-Bonet, J., Marichalar-Mendia, X., & Lertxundi-Manterola, A. (2022). Childhood dental caries experience in northern Spain: a cross-sectional study. Eur Arch Paediatr Dent. https://doi.org/10.1007/s40368-022-00762-2
  • Folayan, M. O., Adeniyi, A. A., Arowolo, O., Maureen, C. N., Alade, M. A., & Tantawi, M. E. (2022). Risk indicators for dental caries, and gingivitis among 6-11-year-old children in Nigeria: a household-based survey. BMC Oral Health,22(1), 465. https://doi.org/10.1186/s12903-022-02470-1
  • Garcia-Rey, S., Ojeda, E., Gunatilake, U. B., Basabe-Desmonts, L., & Benito-Lopez, F. (2021). Alginate Bead Biosystem for the Determination of Lactate in Sweat Using Image Analysis. Biosensors (Basel),11(10). https://doi.org/10.3390/bios11100379
  • Goode, J. A., Rushworth, J. V., & Millner, P. A. (2015). Biosensor Regeneration: A Review of Common Techniques and Outcomes. Langmuir,31(23), 6267-6276. https://doi.org/10.1021/la503533g
  • Hamilton, S., Shea, D., Ibsen, S., & Brasino, M. (2022). On-chip dielectrophoretic recovery and detection of a lactate sensing probiotic from model human saliva. Electrophoresis. https://doi.org/10.1002/elps.202200214
  • Kaur, H., Kumar, R., Babu, J. N., & Mittal, S. (2015). Advances in arsenic biosensor development--a comprehensive review. Biosens Bioelectron,63, 533-545. https://doi.org/10.1016/j.bios.2014.08.003
  • Kimmie-Dhansay, F., & Bhayat, A. (2022). Prevalence of dental caries in the permanent dentition amongst 12-year-olds in Africa: a systematic review and meta-analysis. BMC Oral Health,22(1), 453. https://doi.org/10.1186/s12903-022-02489-4
  • Korona-Glowniak, I., Skawinska-Bednarczyk, A., Wrobel, R., Pietrak, J., Tkacz-Ciebiera, I., Maslanko-Switala, M., Krawczyk, D., Bakiera, A., Borek, A., Malm, A., & Mielnik-Blaszczak, M. (2022). Streptococcus sobrinus as a Predominant Oral Bacteria Related to the Occurrence of Dental Caries in Polish Children at 12 Years Old. Int J Environ Res Public Health,19(22). https://doi.org/10.3390/ijerph192215005
  • Leonard, E. M., Weaver, F. E., & Nurse, C. A. (2022). Lactate sensing by neuroepithelial cells isolated from the gills of killifish (Fundulus heteroclitus). J Exp Biol. https://doi.org/10.1242/jeb.245088Matthews, P. (2022). Prevention of dental caries in children and young people. Nurs Child Young People. https://doi.org/10.7748/ncyp.2022.e145
  • 1Mirzaei, F., Mirzaei, M., & Torkzadeh-Mahani, M. (2019). A hydrophobin-based-biosensor layered by an immobilized lactate dehydrogenase enzyme for electrochemical determination of pyruvate. Bioelectrochemistry,130, 107323. https://doi.org/10.1016/j.bioelechem.2019.06.008
  • Molina, G., Zar, M., Dougall, A., & McGrath, C. (2022). Management of dental caries lesions in patients with disabilities: Update of a systematic review. Front Oral Health,3, 980048. https://doi.org/10.3389/froh.2022.980048
  • Moriyama, C. M., Velasco, S. R. M., Butini, L., Abanto, J., Antunes, J. L. F., & Bonecker, M. (2022). How oral health literacy and parental behavior during the meals relate to dental caries in children. Braz Oral Res,36,e131. https://doi.org/10.1590/1807-3107bor-2022.vol36.0131
  • Nizami, M. Z. I., Yin, I. X., Lung, C. Y. K., Niu, J. Y., Mei, M. L., & Chu, C. H. (2022). In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review. Pharmaceutics,14(10). https://doi.org/10.3390/pharmaceutics14101997
  • Okolo, C. C., Oredugba, F. A., Denloye, O. O., & Adeyemo, Y. I. (2022). Dental Caries, Traumatic Dental Injuries and Gingivitis among Street-Children in Kano, Nigeria. West Afr J Med,39(10), 1040-1044. https://www.ncbi.nlm.nih.gov/pubmed/36260498
  • Olaetxea, I., Lopez, E., Valero, A., & Seifert, A. (2019). Determination of physiological lactate and pH by Raman spectroscopy. Annu Int Conf IEEE Eng Med Biol Soc,475-481. https://doi.org/10.1109/EMBC.2019.8856471
  • Tsvik, L., Steiner, B., Herzog, P., Haltrich, D., & Sutzl, L. (2022). Flavin Mononucleotide-Dependent l-Lactate Dehydrogenases: Expanding the Toolbox of Enzymes for l-Lactate Biosensors. ACS Omega,7(45), 41480-41492. https://doi.org/10.1021/acsomega.2c05257
  • Wei, Y., Wang, J., Dai, D., Wang, H., Zhang, M., Zhang, Z., Zhou, X., He, L., & Cheng, L. (2022). Application of a Caries Treatment Difficulty Assessment System in Dental Caries Management. Int J Environ Res Public Health,19(21). https://doi.org/10.3390/ijerph192114069
  • Zhang, J. S., Chu, C. H., & Yu, O. Y. (2022). Oral Microbiome and Dental Caries Development. Dent J (Basel),10(10). https://doi.org/10.3390/dj10100184
  • Zhang, S., Chen, Y. C., Riezk, A., Ming, D., Tsvik, L., Sutzl, L., Holmes, A., & O'Hare, D. (2022). Rapid Measurement of Lactate in the Exhaled Breath Condensate: Biosensor Optimization and In-Human Proof of Concept. ACS Sens. https://doi.org/10.1021/acssensors.2c01739
  • Zhou, Y., Qi, M., & Yang, M. (2022). Fluorescence determination of lactate dehydrogenase activity based on silicon quantum dots. Spectrochim Acta A Mol Biomol Spectrosc,268,120697. https://doi.org/10.1016/j.saa.2021.120697

Yıl 2023, Cilt: 1 Sayı: 2, 55 - 63, 30.04.2023

Umut Kökbaş

https://doi.org/10.58241/ksbd.6

Öz

Kaynakça

  • Assiri, S. A., El Meligy, O., Alzain, I. O., & Bamashmous, N. O. (2022). Assessment of dental caries and salivary characteristics among type 1 diabetic Saudi children. J Dent Sci,17(4), 1634-1639. https://doi.org/10.1016/j.jds.2022.03.010
  • Benn, A. M. L., Heng, N. C. K., Thomson, W. M., & Broadbent, J. M. (2022). Plaque and dental caries risk in mid-life. Caries Res. https://doi.org/10.1159/000527255
  • Borg-Bartolo, R., Roccuzzo, A., Molinero-Mourelle, P., Schimmel, M., Gambetta-Tessini, K., Chaurasia, A., Koca-Unsal, R. B., Tennert, C., Giacaman, R., & Campus, G. (2022). Global prevalence of edentulism and dental caries in middle-aged and elderly persons: A systematic review and meta-analysis. J Dent,127,104335. https://doi.org/10.1016/j.jdent.2022.104335
  • Chaudhry, S., Haroon, F., Irfan Waheed, K. A., Victor, G., Shahzad, M., & Fatima, B. (2022). Blood Lactate Levels And Lactate Clearance As Predictors Of Mortality In Neonatal Sepsis. J Ayub Med Coll Abbottabad,34(3), 438-441. https://doi.org/10.55519/JAMC-03-9087
  • Fernandez-Bonet, J., Marichalar-Mendia, X., & Lertxundi-Manterola, A. (2022). Childhood dental caries experience in northern Spain: a cross-sectional study. Eur Arch Paediatr Dent. https://doi.org/10.1007/s40368-022-00762-2
  • Folayan, M. O., Adeniyi, A. A., Arowolo, O., Maureen, C. N., Alade, M. A., & Tantawi, M. E. (2022). Risk indicators for dental caries, and gingivitis among 6-11-year-old children in Nigeria: a household-based survey. BMC Oral Health,22(1), 465. https://doi.org/10.1186/s12903-022-02470-1
  • Garcia-Rey, S., Ojeda, E., Gunatilake, U. B., Basabe-Desmonts, L., & Benito-Lopez, F. (2021). Alginate Bead Biosystem for the Determination of Lactate in Sweat Using Image Analysis. Biosensors (Basel),11(10). https://doi.org/10.3390/bios11100379
  • Goode, J. A., Rushworth, J. V., & Millner, P. A. (2015). Biosensor Regeneration: A Review of Common Techniques and Outcomes. Langmuir,31(23), 6267-6276. https://doi.org/10.1021/la503533g
  • Hamilton, S., Shea, D., Ibsen, S., & Brasino, M. (2022). On-chip dielectrophoretic recovery and detection of a lactate sensing probiotic from model human saliva. Electrophoresis. https://doi.org/10.1002/elps.202200214
  • Kaur, H., Kumar, R., Babu, J. N., & Mittal, S. (2015). Advances in arsenic biosensor development--a comprehensive review. Biosens Bioelectron,63, 533-545. https://doi.org/10.1016/j.bios.2014.08.003
  • Kimmie-Dhansay, F., & Bhayat, A. (2022). Prevalence of dental caries in the permanent dentition amongst 12-year-olds in Africa: a systematic review and meta-analysis. BMC Oral Health,22(1), 453. https://doi.org/10.1186/s12903-022-02489-4
  • Korona-Glowniak, I., Skawinska-Bednarczyk, A., Wrobel, R., Pietrak, J., Tkacz-Ciebiera, I., Maslanko-Switala, M., Krawczyk, D., Bakiera, A., Borek, A., Malm, A., & Mielnik-Blaszczak, M. (2022). Streptococcus sobrinus as a Predominant Oral Bacteria Related to the Occurrence of Dental Caries in Polish Children at 12 Years Old. Int J Environ Res Public Health,19(22). https://doi.org/10.3390/ijerph192215005
  • Leonard, E. M., Weaver, F. E., & Nurse, C. A. (2022). Lactate sensing by neuroepithelial cells isolated from the gills of killifish (Fundulus heteroclitus). J Exp Biol. https://doi.org/10.1242/jeb.245088Matthews, P. (2022). Prevention of dental caries in children and young people. Nurs Child Young People. https://doi.org/10.7748/ncyp.2022.e145
  • 1Mirzaei, F., Mirzaei, M., & Torkzadeh-Mahani, M. (2019). A hydrophobin-based-biosensor layered by an immobilized lactate dehydrogenase enzyme for electrochemical determination of pyruvate. Bioelectrochemistry,130, 107323. https://doi.org/10.1016/j.bioelechem.2019.06.008
  • Molina, G., Zar, M., Dougall, A., & McGrath, C. (2022). Management of dental caries lesions in patients with disabilities: Update of a systematic review. Front Oral Health,3, 980048. https://doi.org/10.3389/froh.2022.980048
  • Moriyama, C. M., Velasco, S. R. M., Butini, L., Abanto, J., Antunes, J. L. F., & Bonecker, M. (2022). How oral health literacy and parental behavior during the meals relate to dental caries in children. Braz Oral Res,36,e131. https://doi.org/10.1590/1807-3107bor-2022.vol36.0131
  • Nizami, M. Z. I., Yin, I. X., Lung, C. Y. K., Niu, J. Y., Mei, M. L., & Chu, C. H. (2022). In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review. Pharmaceutics,14(10). https://doi.org/10.3390/pharmaceutics14101997
  • Okolo, C. C., Oredugba, F. A., Denloye, O. O., & Adeyemo, Y. I. (2022). Dental Caries, Traumatic Dental Injuries and Gingivitis among Street-Children in Kano, Nigeria. West Afr J Med,39(10), 1040-1044. https://www.ncbi.nlm.nih.gov/pubmed/36260498
  • Olaetxea, I., Lopez, E., Valero, A., & Seifert, A. (2019). Determination of physiological lactate and pH by Raman spectroscopy. Annu Int Conf IEEE Eng Med Biol Soc,475-481. https://doi.org/10.1109/EMBC.2019.8856471
  • Tsvik, L., Steiner, B., Herzog, P., Haltrich, D., & Sutzl, L. (2022). Flavin Mononucleotide-Dependent l-Lactate Dehydrogenases: Expanding the Toolbox of Enzymes for l-Lactate Biosensors. ACS Omega,7(45), 41480-41492. https://doi.org/10.1021/acsomega.2c05257
  • Wei, Y., Wang, J., Dai, D., Wang, H., Zhang, M., Zhang, Z., Zhou, X., He, L., & Cheng, L. (2022). Application of a Caries Treatment Difficulty Assessment System in Dental Caries Management. Int J Environ Res Public Health,19(21). https://doi.org/10.3390/ijerph192114069
  • Zhang, J. S., Chu, C. H., & Yu, O. Y. (2022). Oral Microbiome and Dental Caries Development. Dent J (Basel),10(10). https://doi.org/10.3390/dj10100184
  • Zhang, S., Chen, Y. C., Riezk, A., Ming, D., Tsvik, L., Sutzl, L., Holmes, A., & O'Hare, D. (2022). Rapid Measurement of Lactate in the Exhaled Breath Condensate: Biosensor Optimization and In-Human Proof of Concept. ACS Sens. https://doi.org/10.1021/acssensors.2c01739
  • Zhou, Y., Qi, M., & Yang, M. (2022). Fluorescence determination of lactate dehydrogenase activity based on silicon quantum dots. Spectrochim Acta A Mol Biomol Spectrosc,268,120697. https://doi.org/10.1016/j.saa.2021.120697
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Halk Sağlığı (Diğer)
Bölüm Araştırma Makalesi
Yazarlar

Umut Kökbaş 0000-0003-4028-3458

Yayımlanma Tarihi 30 Nisan 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 1 Sayı: 2

Kaynak Göster

APA Kökbaş, U. (2023). A Preliminary Study of A Lactic Acid Biosensor for the Early Detection of Dental Caries. Kapadokya Sağlık Bilimleri Dergisi, 1(2), 55-63. https://doi.org/10.58241/ksbd.6
 Kapak Resmi İndir