Öz
Accurate and quick identification of bacterial fish disease has great importance for effective disease control and prevent economic loss in aquaculture facilities. Generally used agar media allow the growth of wide-range bacteria but new chromogenic media results in colored colonies that lead to presumptive identification without requiring biochemical or serological tests. In this study, the recently commercialized chromogenic media; chromID® CPS® Elite, chromID® CPS® Elite / Columbia CNA +5% sheep blood agar, chromID® Vibrio, chromID® MRSA Smart and chromID® Staphylococcus aureus Elite (BioMérieux, France) were tested with growth performance and colony coloration of bacterial fish pathogens. chromID® CPS® Elite and chromID® CPS® Elite / Columbia CNA +5% sheep blood agar were detected the most suitable culture media to grow for all five isolated fish pathogens (i.e., Aeromonas hydrophila, Lactococcus garvieae, Pseudomonas fluorescens, Yersinia ruckeri and Vibrio anguillarum). Of which, L. garvieae was the only one observed with green colonies. chromID® Vibrio designed for V. parahaemolyticus and V cholerae was lead dark pink/purple colonies by V. anguillarum. The chromID® MRSA Smart and chromID® S. aureus Elite were detected not effective for incubation of bacterial fish pathogens.
Anahtar Kelimeler
Destekleyen Kurum
Izmir Katip Celebi University Scientific Research Projects Coordinatorship
Proje Numarası
2017-GAP-SUÜF-0005
Kaynakça
- Algöet M, Bayley AE, Roberts EG, Feist SW, Wheeler RW, Verner‐Jeffreys DW. 2009. Susceptibility of selected freshwater fish species to a UK Lactococcus garvieae isolate. J Fish Dis. 32(10):825-834. doi: 10.1111/j.1365-2761.2009.01058.x
- Alrabadi NI. 2012. The effect of several antibiotics on Lactococcus garvieae isolated from Jordanian dairy products. Am J Agric Biol Sci. 7(4):468-472. doi: 10.3844/ajabssp.2012.468.472
- Austin B, Austin DA. 2007. Bacterial Fish Pathogens 4th ed. the UK: Springer 552 p.
- Balta F, Dengiz Balta Z. 2019. The Isolation of Lactococcus garvieae from Eyes of Diseased Rainbow Trout (Oncorhynchus mykiss) with Exopthalmia. J Anatolian Env and Anim Sciences. 4(1):27-33. doi: 10.35229/jaes.527258
- Blanco-Abad V, Ansede-Bermejo J, Rodrıguez-Castro A, Martinez-Urtaza J. 2009. Evaluation of different procedures for the optimized detection of Vibrio parahaemolyticus in mussels and environmental samples. Int J Food Microbiol. 129(3):229-236. doi: 10.1016/j.ijfoodmicro.2008.11.028
- Cabello FC, Godfrey HP, Tomova A, Ivanova L, Dölz H, Millanao A, Buschmann AH. 2013. Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health. Environ Microbiol. 15(7):1917-1942. doi: 10.1111/1462-2920.12134
- Eddabra R, Piemont Y, Scheftel JM. 2011. Evaluation of a new chromogenic medium, chromID™ Vibrio, for the isolation and presumptive identification of Vibrio cholerae and Vibrio parahaemolyticus from human clinical specimens. Eur J Clin Microbiol. 30(6):733-737. doi: 10.1007/s10096-010-1145-2
- Farmer JJ, Hickman-Brenner FW, Kelly MT. 1985. Vibrio. In: Lennette EH, Balows A, Hausler Jr WJ, Shadomy HJ, editors. Manual of Clinical Microbiology, 4th ed. Washington DC: American Society for Microbiology Press. p. 282-301.
- Ghittino C, Prearo M. 1992. Report of Streptococcosis in rainbow trout (Oncorhynchus mykiss) in Italy: preliminary note. Boll Soc Ital Patol. 8:4-9.
- Gil P, Vivas J, Gallardo CS, Rodriguez LA. 2000. First isolation of Staphylococcus warneri, from diseased rainbow trout, Oncorhynchus mykiss (Walbaum), in Northwest Spain. J Fish Dis. 23(4):295-298. doi: 10.1046/j.1365-2761.2000.00244.x
- Grimes DJ. 1991. Ecology of estuarine bacteria capable of causing human disease: a review. Estuaries. 14(4):345-360. doi: 10.2307/1352260
- Kayış S, Çapkın E, Balta F, Altınok I. 2009. Bacteria in rainbow trout (Oncorhynchus mykiss) in the Southern Black Sea Region of Turkey - A survey. Isr J Aquacult-Bamid.61(4):339-344. doi: 10.46989/001c.20564
- Muziasari WI, Managaki S, Pärnänen K, Karkman A, Lyra C, Tamminen M, Suzuki S, Virta M. 2014. Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment. PlosOne.9(3):e92702. doi:10.1371/journal.pone.0092702
- Perry JD, Freydière AM. 2007. The application of chromogenic media in clinical microbiology. J Appl Microbiol. 103(6):2046-2055. doi: 10.1111/j.1365-2672.2007.03442.x
- Perry JD. 2017. A decade of development of chromogenic culture media for clinical microbiology in an era of molecular diagnostics. Clin Microbiol Rev. 30(2):449-479. doi: 10.1128/CMR.00097-16
- Pinto A, Terio V, Novello L, Tantillo G. 2011. Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus. Food Control. 22(1):124-127. doi: 10.1016/j.foodcont.2010.06.013
- Shotts EB, Teska JD. 1989. Bacterial pathogens of aquatic vertebrates. In: Austin B, Austin DA, editors. Methods for the Microbiological Examinationof Fish and Shellfish. Oxford: Ellis Horwood Ltd. p. 164-181.
- Tanrıkul TT. 2012. Efficacy of a whole cell Lactococcus garvieae vaccine in rainbow trout (Oncorhynchus mykiss). J Anim Vet Adv. 11(7):886-889. doi: 10.3923/javaa.2012.886.889
- Ture M, Boran H. 2015. Phenotypic and genotypic antimicrobial resistance of Lactococcus sp. strains isolated from rainbow trout (Oncorhynchus mykiss). Bull Vet Inst Pulawy. 59(1):37-42. doi: 10.1515/bvip-2015-0006
- Yarbrough ML, Wallace MA, Marshall C, Mathias E, Burnham CAD. 2016. Culture of urine specimens using chromID CPS elite medium can expedite Escherichia coli identification and reduce hands-on time in the clinical laboratory. J Clin Microbiol. 54(11):2767-2773. doi: 10.1128/JCM.01376-16
Öz
Akuakültür işletmelerinde etkin hastalık kontrolü ve ekonomik kayıpların önlenmesi için bakteriyel balık hastalıklarının doğru ve hızlı tanımlanması büyük önem taşımaktadır. Genel olarak kullanılan agar besiyerlerinde çok çeşitli bakteri türünün üremesi gözlenirken, yeni kromojenik besiyerleri, biyokimyasal ve serolojik test gerektirmeden renkli koloni görünümüyle tahmini ön tanımlama imkânı sunmaktadır. Bu çalışmada yakın zamanda ticarileşen kromojenik besiyerlerinde (chromID® CPS® Elite, chromID® CPS® Elite / Columbia CNA +5% sheep blood agar, chromID® Vibrio, chromID® MRSA Smart ve chromID® Staphylococcus aureus Elite (BioMérieux, France)) bakteriyel balık patojenlerinin üremesi ve koloni renklenmeleri tespit edilmiştir. ChromID® CPS® Elite ve chromID® CPS® Elite / Columbia CNA +5% sheep blood agarın test edilen 5 farklı bakteriyel balık patojeni (Aeromonas hydrophila, Lactococcus garvieae, Pseudomonas fluorescens, Yersinia ruckeri ve Vibrio anguillarum) için en uygun besiyeri olduğu, ancak sadece L. garvieae’nin yeşil koloni görünümü ile ürediği belirlenmiştir. Vibrio parahaemolyticus ve Vibrio cholerae’nın hızlı teşhisi için geliştirilen chromID® Vibrio besiyerinde V. anguillarum kolonileri koyu pembe/mor renkli olarak gözlenmiş, chromID® MRSA Smart ve chromID® S. aureus Elite besiyerlerinin bakteriyel balık patojenlerinin üremesi için uygun olmadığı belirlenmiştir.
Anahtar Kelimeler
Kromojenik besiyeri balık hastalıkları balık patojenleri izolasyon
Proje Numarası
2017-GAP-SUÜF-0005
Kaynakça
- Algöet M, Bayley AE, Roberts EG, Feist SW, Wheeler RW, Verner‐Jeffreys DW. 2009. Susceptibility of selected freshwater fish species to a UK Lactococcus garvieae isolate. J Fish Dis. 32(10):825-834. doi: 10.1111/j.1365-2761.2009.01058.x
- Alrabadi NI. 2012. The effect of several antibiotics on Lactococcus garvieae isolated from Jordanian dairy products. Am J Agric Biol Sci. 7(4):468-472. doi: 10.3844/ajabssp.2012.468.472
- Austin B, Austin DA. 2007. Bacterial Fish Pathogens 4th ed. the UK: Springer 552 p.
- Balta F, Dengiz Balta Z. 2019. The Isolation of Lactococcus garvieae from Eyes of Diseased Rainbow Trout (Oncorhynchus mykiss) with Exopthalmia. J Anatolian Env and Anim Sciences. 4(1):27-33. doi: 10.35229/jaes.527258
- Blanco-Abad V, Ansede-Bermejo J, Rodrıguez-Castro A, Martinez-Urtaza J. 2009. Evaluation of different procedures for the optimized detection of Vibrio parahaemolyticus in mussels and environmental samples. Int J Food Microbiol. 129(3):229-236. doi: 10.1016/j.ijfoodmicro.2008.11.028
- Cabello FC, Godfrey HP, Tomova A, Ivanova L, Dölz H, Millanao A, Buschmann AH. 2013. Antimicrobial use in aquaculture re-examined: its relevance to antimicrobial resistance and to animal and human health. Environ Microbiol. 15(7):1917-1942. doi: 10.1111/1462-2920.12134
- Eddabra R, Piemont Y, Scheftel JM. 2011. Evaluation of a new chromogenic medium, chromID™ Vibrio, for the isolation and presumptive identification of Vibrio cholerae and Vibrio parahaemolyticus from human clinical specimens. Eur J Clin Microbiol. 30(6):733-737. doi: 10.1007/s10096-010-1145-2
- Farmer JJ, Hickman-Brenner FW, Kelly MT. 1985. Vibrio. In: Lennette EH, Balows A, Hausler Jr WJ, Shadomy HJ, editors. Manual of Clinical Microbiology, 4th ed. Washington DC: American Society for Microbiology Press. p. 282-301.
- Ghittino C, Prearo M. 1992. Report of Streptococcosis in rainbow trout (Oncorhynchus mykiss) in Italy: preliminary note. Boll Soc Ital Patol. 8:4-9.
- Gil P, Vivas J, Gallardo CS, Rodriguez LA. 2000. First isolation of Staphylococcus warneri, from diseased rainbow trout, Oncorhynchus mykiss (Walbaum), in Northwest Spain. J Fish Dis. 23(4):295-298. doi: 10.1046/j.1365-2761.2000.00244.x
- Grimes DJ. 1991. Ecology of estuarine bacteria capable of causing human disease: a review. Estuaries. 14(4):345-360. doi: 10.2307/1352260
- Kayış S, Çapkın E, Balta F, Altınok I. 2009. Bacteria in rainbow trout (Oncorhynchus mykiss) in the Southern Black Sea Region of Turkey - A survey. Isr J Aquacult-Bamid.61(4):339-344. doi: 10.46989/001c.20564
- Muziasari WI, Managaki S, Pärnänen K, Karkman A, Lyra C, Tamminen M, Suzuki S, Virta M. 2014. Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment. PlosOne.9(3):e92702. doi:10.1371/journal.pone.0092702
- Perry JD, Freydière AM. 2007. The application of chromogenic media in clinical microbiology. J Appl Microbiol. 103(6):2046-2055. doi: 10.1111/j.1365-2672.2007.03442.x
- Perry JD. 2017. A decade of development of chromogenic culture media for clinical microbiology in an era of molecular diagnostics. Clin Microbiol Rev. 30(2):449-479. doi: 10.1128/CMR.00097-16
- Pinto A, Terio V, Novello L, Tantillo G. 2011. Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus. Food Control. 22(1):124-127. doi: 10.1016/j.foodcont.2010.06.013
- Shotts EB, Teska JD. 1989. Bacterial pathogens of aquatic vertebrates. In: Austin B, Austin DA, editors. Methods for the Microbiological Examinationof Fish and Shellfish. Oxford: Ellis Horwood Ltd. p. 164-181.
- Tanrıkul TT. 2012. Efficacy of a whole cell Lactococcus garvieae vaccine in rainbow trout (Oncorhynchus mykiss). J Anim Vet Adv. 11(7):886-889. doi: 10.3923/javaa.2012.886.889
- Ture M, Boran H. 2015. Phenotypic and genotypic antimicrobial resistance of Lactococcus sp. strains isolated from rainbow trout (Oncorhynchus mykiss). Bull Vet Inst Pulawy. 59(1):37-42. doi: 10.1515/bvip-2015-0006
- Yarbrough ML, Wallace MA, Marshall C, Mathias E, Burnham CAD. 2016. Culture of urine specimens using chromID CPS elite medium can expedite Escherichia coli identification and reduce hands-on time in the clinical laboratory. J Clin Microbiol. 54(11):2767-2773. doi: 10.1128/JCM.01376-16
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Proje Numarası | 2017-GAP-SUÜF-0005 |
| Yayımlanma Tarihi | 26 Ağustos 2021 |
| Yayımlandığı Sayı | Yıl 2021 Cilt: 7 Sayı: 2 |