Antibacterial Activity of Laetiporus sulphureus Extracts Against Multidrug Resistant Bacterial Isolates
Öz
Ethanol extracts from Laetiporus sulphureus (Bull.) Murrill (Polyporaceae) were tested for their antibacterial activity against multidrug-resistant bacterial isolates using agar well diffusion and dilution procedures. According to the findings, L. sulphureus's antibacterial activity differed depending on the extract concentration. While no activity was observed at other dosages, the ethanolic extract demonstrated inhibitory zones ranging from 10.2 to 17.4 mm at 200 mg/mL; 8.2 to 12.6 mm at 100 mg/mL; and 9.2 to 9.8 mm at 50 mg/mL. Klebsiella pneumoniae (10.2 mm at 200 mg/mL) was the least susceptible to the extract, while Bacillus subtilis (17.4 mm at 200 mg/mL) was the most sensitive. The extract’s minimum inhibitory concentration (MIC) against Staphylococcus aureus, B. subtilis, and K. pneumoniae ranged between 25 mg/mL and 200 mg/mL. For B. subtilis, S. aureus, and Pseudomonas aeruginosa, the minimum bactericidal concentration (MBC) of the extract was recorded at 50 mg/mL and 200 mg/mL, respectively; however, no MBC was found for K. pneumoniae. S. aureus exhibited the highest level of resistance (70%), while P. aeruginosa demonstrated the lowest level of resistance (10%). Pefloxacin, ciprofloxacin, septrin, and sparfloxacin were identified as the most potent antibiotics. The findings suggest that L. sulphureus has potential as an antibacterial agent, though its effectiveness varies according to extract concentration and bacterial species.
Anahtar Kelimeler
Laetiporus sulphureus Antibacterial Activity Multidrug-Resistant Bacterial Isolates
Kaynakça
- Acharya, K., Ghosh, S., Khatua, S.,and Mitra, P. (2016). Pharmacognostic Standardization and Antioxidant Capacity of An Edible Mushroom Laetiporus sulphureus. Journal für Verbraucherschutz und Lebensmittelsicherheit,11: 33-42. https://doi.org:10.1007/s00003-015-0977-1
- Ahmad, I.,and Beg, A.Z. (2001). Antimicrobial and Phytochemical Studies on 45 Indian Medicinal Plants Against Multi-drug Resistant Human Pathogens. Journal of Ethnopharmacology, 74: 113-123. https://doi.org:10.1016/S0378-8741(00)00335-4
- Ajaiyeoba, E.O., Onocha, P.A., Nwoza, S.O., and Soma, W. (2003). Antimicrobial and Cytotoxicity Evaluation of Buchlolzia curiacea Stembark. Phytotherapy, 74: 706-709. https://doi.org:10.1016/s0367-326x(03)00142-4
- Alves, M.J., Ferreira, I.C.F.R., Dias, J., Teiceira, V., Martins, A., and Pitado, M. (2012). A Review on Antimicrobial Activity of Mushroom (Basidiomyctes) Extracts and Isolated Compounds. Planta Medica, 78: 1707-1718. https://doi.org:10.1055/s-0032-1315370
- Brochers, A., Keen, C.L., and Gershwin, M.E. (2004). Mushrooms, Tumors, and Immunity: An Update. Experimental Biology and Medicine, 229: 393-406. https://doi.org: 10.1177/153537020422900507
- Duvnjak, D., Pantic, M., Pavlovic, V., Nedovic, V., Levic, S., Matijasevic, D., Sknepnek, A., and Niksic, M. (2016). Advances in Batch Culture Fermented Coriolus versicolor Medicinal Mushroom for the Production of Antibacterial Compounds. Innovative Food Science and Emerging Technologies, 34: 1-8. https://doi.org:10.1016/j.ifset.2015.12.028
- Fan, Q., Yin, X., Li, Z., Li, Y., Liu, J., Feng, T., and Zhao, A. (2014). Mycophenolic Acid Derivatives from Cultures of the Mushroom Laetiporus sulphureus. Chinese Journal of Natural Medicines, 12: 685-688. https://doi.org: 10.1016/S1875-5364(14)60104-7
- Grienke, U., Zoll, M., Peintner, U., and Rollinger, J.M. (2014). European Medicinal Polypores – A Modern View on Traditional Uses. Journal of Ethnopharmacology, 154: 564-583 https://doi.org: 10.1016/j.jep.2014.04.030.
- Kalac, P. (2009). Chemical Composition and Nutritional Value of European Species of Wild Growing Mushrooms: A Review. Food Chemistry, 113:9-16. https://doi.org: 10.1016/j.foodchem.2008.07.077
- Khatua, S., Ghosh, S., and Acharya, K. (2017). Laetiporus sulphureus (Bull.:Fr.) Murr. As Food As Medicine. Pharmacognosy Journal, 9(6): 1-15 suppl. https://doi.org: 10.5530/pj.2017.6s.151
- Li, W.R., Xie, X.B., Shi, Q.S., Zeng, H.Y., Ou-Yang, Y.S., and Chen, Y.B. (2010). Antibacterial Activity and Mechanism of Silver Nanoparticles on Escherichia coli.Applied Microbiology and Biotechnology, 85: 1115-1122. https://doi.org: 10.1007/s00253-009-2159-5
- Lindequist, U., Niedermeyer, T.H.J., and Julich, W.D. (2005). The Pharmacological Potential of Mushrooms. Evidence-Based Complementary and Alternative Medicine (eCAM), 2: 285-299. https://doi.org:10.1093/ecam/neh107
- NCCLS (2000). National Committee for Clinical Laboratory Standards (NCCLS). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically: Approved Standards – fifth edition. NCCLS Document M7-A5. NCCLS: Wayne, PA, USA.
- Parvu, M., Andrei, A., and Rosca-Casian, O. (2010). Antifungal Activity of Laetiporus sulphureus Mushroom Extract. Contributii Botanice, XLV: 65-70.
- Petrovic, J., Glamoclija, J., Stojkovic, D.S., Civic, A., Nikolic, M., Bukvicki, D., Guerzoni, M.E., and Sokovic, M.D. (2013). Laetiporus sulphureus, Edible Mushroom from Serbia: Investigation on Volatile Compounds in vitro Antimicrobial Activity and in situ Control of Aspergillus flavus in Tomato Paste. Food and Chemical Toxicology, 59: 297-302. https://doi.org: 10.1016/j.fct.2013.06.021
- Poucheret, P., Fons, F., and Rapior, S. (2006). Biological and Pharmacological Activity of Higher Fungi: 20 Years Retrospective Analysis. Cryptogamie, Mycologie, 27: 311-333.
- Ren, L., Hemar, Y., Perera, C.O., Lewis, G., Krissansen, G.W., and Buchanon, P.K. (2014). Antibacterial and Antioxidant Activities of Aqueous Extracts of Eight Mushrooms. Bioactive Carbohydrates and Dietary Fibre,3: 41-51. https://doi.org:10.1016/j.bcdf.2014.01.003
- Sesli, E., Asan, A. ve Selçuk, F. (edlr.) Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H.H., Erdoğdu, M., Ergül, C.C., Eroğlu, G., Giray, G., Halikî Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Ya., Uzun, Yu., Yoltaş, A. (2020). Türkiye Mantarları Listesi. İstanbul: Ali Nihat Gökyiğit Vakfı Yayınları.
- Sinanoglu, V.J., Zoumpoulakis, P., Heropoulos, G., Proestos, C., Ciric, A., Petrovic, I., Glamoclija, J., and Sokovic,M. (2015). Lipid and Fatty Acid Profile of the Edible Fungus Laetiporus sulphureus. Antifungal and Antibacterial Properties. Journal of Food Science and Technology, 52(6): 3264-3272. https://doi.org:10.1007/s13197-014-1377-8.
- Turkoglu, A., Duru, M.E., Mercan, N., Kıvrak, I., and Gezer, K. (2007). Antioxidant and Antimicrobial Activities of Laetiporus sulphureus (Bull.) Murrill. Food chemistry, 101: 267-273. https://doi.org/10.1016/j.foodchem.2006.01.025.
- Wang, J., Sun, W., Luo, H., He, H., Deng, W., Zou, K., Liu, C., Song, J., and Huang, W. (2015). Protective Effect of Eburicoic Acid of the Chicken of the Woods Mushroom, Laetiporus sulphureus (higher basidiomycetes), against gastric ulcers in mice. International Journal of Medicinal Mushroom, 17: 619-626.https://doi.org:10.1615/intjmedmushrooms.v17.i7.20
- Younis, A.M., Yosri, M., and Stewart, J.K. (2019). In vitro Evaluation of Pleiotropic Properties of Wild Mushroom Laetiporus sulphureus. Annals of Agricultural Sciences, 64: 79-87. https://doi.org/10.1016/j.aoas.2019.05.001
- Zjawiony, J.K. (2004). Biologically Active Compounds from Aphyllophorales (Polypore) Fungi. Journal of Natural Products, 67(2): 300-310. https://doi.org:10.1021/np030372w
Çoklu İlaca Dirençli Bakteri İzolatlarına Karşı Laetiporus sulphureus Ekstraktlarının Antibakteriyel Aktivitesi
Öz
Laetiporus sulphureus (Bull.) Murrill (Polyporaceae) etanol ekstraktlarının, çoklu ilaca dirençli bakteri izolatlarına karşı antibakteriyel aktivitesi agar kuyucuk difüzyon ve dilüsyon yöntemleri kullanılarak test edilmiştir. Bulgulara göre, L. sulphureus'un antibakteriyel aktivitesi ekstrakt konsantrasyonuna bağlı olarak değişiklik göstermektedir. Diğer dozlarda aktivite gözlenmezken, etanolik ekstrakt 200 mg/mL'de 10,2 ile 17,4 mm arasında, 100 mg/mL'de 8,2 ile 12,6 mm arasında ve 50 mg/mL'de 9,2 ile 9,8 mm arasında inhibisyon zonları göstermiştir. Ekstrakta en az duyarlı bakteri 200 mg/mL'de 10,2 mm ile Klebsiella pneumoniae iken, en duyarlı bakteri 200 mg/mL'de 17,4 mm ile Bacillus subtilis olmuştur. Ekstraktın Staphylococcus aureus, B. subtilis ve K. pneumoniae'ye karşı minimum inhibitör konsantrasyonu (MİK) 25 mg/mL ile 200 mg/mL arasında değişmektedir. B. subtilis, S. aureus ve Pseudomonas aeruginosa için ekstraktın minimum bakterisidal konsantrasyonu (MBK) sırasıyla 50 mg/mL ve 200 mg/mL olarak kaydedilmiştir; ancak K. pneumoniae için herhangi bir MBK bulunamamıştır. S. aureus en yüksek direnç seviyesini (%70) gösterirken, P. aeruginosa en düşük direnç seviyesini (%10) göstermiştir. Pefloksasin, siprofloksasin, septrin ve sparfloksasin en güçlü antibiyotikler olarak tanımlanmıştır. Bulgular, L. sulphureus'un bir antibakteriyel ajan olarak potansiyel taşıdığını, ancak etkinliğinin ekstrakt konsantrasyonuna ve bakteri türüne göre değiştiğini göstermektedir.
Anahtar Kelimeler
Laetiporus sulphureus Antibakteriyel Aktivite Çoklu İlaca Dirençli Bakteri İzolatları
Kaynakça
- Acharya, K., Ghosh, S., Khatua, S.,and Mitra, P. (2016). Pharmacognostic Standardization and Antioxidant Capacity of An Edible Mushroom Laetiporus sulphureus. Journal für Verbraucherschutz und Lebensmittelsicherheit,11: 33-42. https://doi.org:10.1007/s00003-015-0977-1
- Ahmad, I.,and Beg, A.Z. (2001). Antimicrobial and Phytochemical Studies on 45 Indian Medicinal Plants Against Multi-drug Resistant Human Pathogens. Journal of Ethnopharmacology, 74: 113-123. https://doi.org:10.1016/S0378-8741(00)00335-4
- Ajaiyeoba, E.O., Onocha, P.A., Nwoza, S.O., and Soma, W. (2003). Antimicrobial and Cytotoxicity Evaluation of Buchlolzia curiacea Stembark. Phytotherapy, 74: 706-709. https://doi.org:10.1016/s0367-326x(03)00142-4
- Alves, M.J., Ferreira, I.C.F.R., Dias, J., Teiceira, V., Martins, A., and Pitado, M. (2012). A Review on Antimicrobial Activity of Mushroom (Basidiomyctes) Extracts and Isolated Compounds. Planta Medica, 78: 1707-1718. https://doi.org:10.1055/s-0032-1315370
- Brochers, A., Keen, C.L., and Gershwin, M.E. (2004). Mushrooms, Tumors, and Immunity: An Update. Experimental Biology and Medicine, 229: 393-406. https://doi.org: 10.1177/153537020422900507
- Duvnjak, D., Pantic, M., Pavlovic, V., Nedovic, V., Levic, S., Matijasevic, D., Sknepnek, A., and Niksic, M. (2016). Advances in Batch Culture Fermented Coriolus versicolor Medicinal Mushroom for the Production of Antibacterial Compounds. Innovative Food Science and Emerging Technologies, 34: 1-8. https://doi.org:10.1016/j.ifset.2015.12.028
- Fan, Q., Yin, X., Li, Z., Li, Y., Liu, J., Feng, T., and Zhao, A. (2014). Mycophenolic Acid Derivatives from Cultures of the Mushroom Laetiporus sulphureus. Chinese Journal of Natural Medicines, 12: 685-688. https://doi.org: 10.1016/S1875-5364(14)60104-7
- Grienke, U., Zoll, M., Peintner, U., and Rollinger, J.M. (2014). European Medicinal Polypores – A Modern View on Traditional Uses. Journal of Ethnopharmacology, 154: 564-583 https://doi.org: 10.1016/j.jep.2014.04.030.
- Kalac, P. (2009). Chemical Composition and Nutritional Value of European Species of Wild Growing Mushrooms: A Review. Food Chemistry, 113:9-16. https://doi.org: 10.1016/j.foodchem.2008.07.077
- Khatua, S., Ghosh, S., and Acharya, K. (2017). Laetiporus sulphureus (Bull.:Fr.) Murr. As Food As Medicine. Pharmacognosy Journal, 9(6): 1-15 suppl. https://doi.org: 10.5530/pj.2017.6s.151
- Li, W.R., Xie, X.B., Shi, Q.S., Zeng, H.Y., Ou-Yang, Y.S., and Chen, Y.B. (2010). Antibacterial Activity and Mechanism of Silver Nanoparticles on Escherichia coli.Applied Microbiology and Biotechnology, 85: 1115-1122. https://doi.org: 10.1007/s00253-009-2159-5
- Lindequist, U., Niedermeyer, T.H.J., and Julich, W.D. (2005). The Pharmacological Potential of Mushrooms. Evidence-Based Complementary and Alternative Medicine (eCAM), 2: 285-299. https://doi.org:10.1093/ecam/neh107
- NCCLS (2000). National Committee for Clinical Laboratory Standards (NCCLS). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically: Approved Standards – fifth edition. NCCLS Document M7-A5. NCCLS: Wayne, PA, USA.
- Parvu, M., Andrei, A., and Rosca-Casian, O. (2010). Antifungal Activity of Laetiporus sulphureus Mushroom Extract. Contributii Botanice, XLV: 65-70.
- Petrovic, J., Glamoclija, J., Stojkovic, D.S., Civic, A., Nikolic, M., Bukvicki, D., Guerzoni, M.E., and Sokovic, M.D. (2013). Laetiporus sulphureus, Edible Mushroom from Serbia: Investigation on Volatile Compounds in vitro Antimicrobial Activity and in situ Control of Aspergillus flavus in Tomato Paste. Food and Chemical Toxicology, 59: 297-302. https://doi.org: 10.1016/j.fct.2013.06.021
- Poucheret, P., Fons, F., and Rapior, S. (2006). Biological and Pharmacological Activity of Higher Fungi: 20 Years Retrospective Analysis. Cryptogamie, Mycologie, 27: 311-333.
- Ren, L., Hemar, Y., Perera, C.O., Lewis, G., Krissansen, G.W., and Buchanon, P.K. (2014). Antibacterial and Antioxidant Activities of Aqueous Extracts of Eight Mushrooms. Bioactive Carbohydrates and Dietary Fibre,3: 41-51. https://doi.org:10.1016/j.bcdf.2014.01.003
- Sesli, E., Asan, A. ve Selçuk, F. (edlr.) Abacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H.H., Erdoğdu, M., Ergül, C.C., Eroğlu, G., Giray, G., Halikî Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Ya., Uzun, Yu., Yoltaş, A. (2020). Türkiye Mantarları Listesi. İstanbul: Ali Nihat Gökyiğit Vakfı Yayınları.
- Sinanoglu, V.J., Zoumpoulakis, P., Heropoulos, G., Proestos, C., Ciric, A., Petrovic, I., Glamoclija, J., and Sokovic,M. (2015). Lipid and Fatty Acid Profile of the Edible Fungus Laetiporus sulphureus. Antifungal and Antibacterial Properties. Journal of Food Science and Technology, 52(6): 3264-3272. https://doi.org:10.1007/s13197-014-1377-8.
- Turkoglu, A., Duru, M.E., Mercan, N., Kıvrak, I., and Gezer, K. (2007). Antioxidant and Antimicrobial Activities of Laetiporus sulphureus (Bull.) Murrill. Food chemistry, 101: 267-273. https://doi.org/10.1016/j.foodchem.2006.01.025.
- Wang, J., Sun, W., Luo, H., He, H., Deng, W., Zou, K., Liu, C., Song, J., and Huang, W. (2015). Protective Effect of Eburicoic Acid of the Chicken of the Woods Mushroom, Laetiporus sulphureus (higher basidiomycetes), against gastric ulcers in mice. International Journal of Medicinal Mushroom, 17: 619-626.https://doi.org:10.1615/intjmedmushrooms.v17.i7.20
- Younis, A.M., Yosri, M., and Stewart, J.K. (2019). In vitro Evaluation of Pleiotropic Properties of Wild Mushroom Laetiporus sulphureus. Annals of Agricultural Sciences, 64: 79-87. https://doi.org/10.1016/j.aoas.2019.05.001
- Zjawiony, J.K. (2004). Biologically Active Compounds from Aphyllophorales (Polypore) Fungi. Journal of Natural Products, 67(2): 300-310. https://doi.org:10.1021/np030372w
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Mikoloji |
| Bölüm | ARAŞTIRMA MAKALESİ |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Nisan 2025 |
| Gönderilme Tarihi | 30 Ekim 2024 |
| Kabul Tarihi | 19 Kasım 2024 |
| Yayımlandığı Sayı | Yıl 2025 Cilt: 16 Sayı: 1 |
Uluslararası Hakemli Dergi
Dergimiz, herhangi bir başvuru veya yayımlama ücreti almamaktadır
