Determination of Trichomonas vaginalis Frequency Among SymptomaticCases in Muğla Sıtkı Koçman Hospital Using Different Methods (Direct Microscopy, Culture, PCR and Immunochromatographic Method)

Funda Sankur; Sema Ertuğ; Erdoğan Malatyalı; Evren Tileklioğlu; İbrahim Yıldız; Hatice Ertabaklar

doi:10.4274/meandros.galenos.2023.04557

Araştırma Makalesi

Yıl 2023, Cilt: 24 Sayı: 4, 321 - 326, 30.12.2023

Funda Sankur Sema Ertuğ , Erdoğan Malatyalı , Evren Tileklioğlu İbrahim Yıldız Hatice Ertabaklar

Öz

Kaynakça

1. Kissinger P. Trichomonas vaginalis: a review of epidemiologic, clinical and treatment issues. BMC Infect Dis 2015; 15: 307.
2. Thorburn AL. Alfred François Donné, 1801-1878, discoverer of Trichomonas vaginalis and of leukaemia. Br J Vener Dis 1974; 50: 377-80.
3. Rowley J, Vander Hoorn S, Korenromp E, Low N, Unemo M, AbuRaddad LJ, et al. Chlamydia, gonorrhoea, trichomoniasis and syphilis: global prevalence and incidence estimates, 2016. Bull World Health Organ 2019; 97: 548-62.
4. Menezes CB, Frasson AP, Tasca T. Trichomoniasis - are we givingthe deserved attention to the most common non-viral sexuallytransmitted disease worldwide? Microb Cell 2016; 3: 404-19.
5. Crucitti T, Jespers V, Mulenga C, Khondowe S, Vandepitte J, BuvéA. Non-sexual transmission of Trichomonas vaginalis in adolescent girls attending school in Ndola, Zambia. PLoS One 2011; 6: e16310.
6. Meites E, Gaydos CA, Hobbs MM, Kissinger P, Nyirjesy P, SchwebkeJR, et al. A Review of Evidence-Based Care of SymptomaticTrichomoniasis and Asymptomatic Trichomonas vaginalisInfections. Clin Infect Dis 2015; 61(Suppl 8): S837-48.
7. Değerli S, Şalk S, Malatyalı E. Sivas'ta vaginit ön tanılı hastalardaTrichomonas vaginalis sıklığı [Incidence in Sivas of Trichomonasvaginalis in patients with vaginitis]. Turkiye Parazitol Derg 2011; 35: 145-7.
8. Suay A, Yayla M, Mete Ö, Elçi S. 300 Hayat kadınında direkt mikroskobi ve kültür yöntemleriyle Trichomonas vaginalis vebunabağlı olarak Trikomoniyaz’ın araştırılması. Türkiye Parazitol Derg1995; 19: 170-3.
9. Hobbs MM, Seña AC. Modern diagnosis of Trichomonas vaginalisinfection. Sex Transm Infect 2013; 89: 434-8.
10. Fonseca THS, Oliveira FMS, Alacoque M, Rocha MI, LeiteHV, Santos JFG, et al. Immunocytochemistry Improving the Diagnosisof Trichomonas vaginalis Infections. Biomed Res Int 2017; 2017: 5642535.
11. Bhesania AH, Narayankhedkar A. Trichomoniasis-A Review. Int JCurr Microbiol App Sci 2016; 5: 731-41.
12. Lazenby GB. Trichomonas vaginalis screening and preventioninorderto impact the HIV pandemic: Isn't it time we take this infectionseriously? Infect Dis Rep 2011; 3: e4.
13. Fernando SD, Herath S, Rodrigo C, Rajapakse L. Clinical featuresand sociodemographic factors affecting Trichomonas vaginalisinfection in women attending a central sexually transmitteddiseases clinic in Sri Lanka. Indian J Sex TransmDis AIDS2012; 33: 25-31.
14. Garber GE. The laboratory diagnosis of Trichomonas vaginalis. CanJ Infect Dis Med Microbiol 2005; 16: 35-8.
15. Radonjic IV, Dzamic AM, Mitrovic SM, Arsic Arsenijevic VS, PopadicDM, Kranjcic Zec IF. Diagnosis of Trichomonas vaginalis infection: The sensitivities and specificities of microscopy, culture andPCRassay. Eur J Obstet Gynecol Reprod Biol 2006; 126: 116-20.
16. Morris SR, Bristow CC, Wierzbicki MR, Sarno M, Asbel L, FrenchA, et al. Performance of a single-use, rapid, point-of-care PCRdevicefor the detection of Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis: a cross-sectional study. Lancet Infect Dis 2021; 21: 668-76.
17. Madhivanan P, Li T, Trammell S, Desai C, Srinivas V, Arun A, et al. Performance of the OSOM Trichomonas Rapid Test for diagnosisof Trichomonas vaginalis infection among women in Mysore, India. Sex Health 2013; 10: 320-4.
18. Khatoon R, Jahan N, Ahmad S, Khan HM, Rabbani T. Comparisonof four diagnostic techniques for detection of Trichomonas vaginalisinfection in females attending tertiary care hospital of NorthIndia. Indian J Pathol Microbiol 2015; 58: 36-9.
19. Huppert JS, Batteiger BE, Braslins P, Feldman JA, HobbsMM, Sankey HZ, et al. Use of an immunochromatographic assayforrapid detection of Trichomonas vaginalis in vaginal specimens. JClin Microbiol 2005; 43: 684-7.
20. Aycan Kaya Ö, Benk-Şilfeler D, Özgür T, Keskin Kurt R, Hamamcı B, Sefil N, et al. Bir üniversite hastanesi jinekoloji polikliniğinebaşvuran kadınlarda parazitolojik ve sitolojik yöntemlerleTrichomonas vaginalis araştırılması. Konuralp Tıp Dergisi 2015; 7: 146-8.
21. Ostan I, Sözen U, Lımoncu ME, Kılımcıoğlu AA, Ozbılgın A. Manisa'da vajinal akıntılı kadınlarda Trichomonas vaginalis sıklığı [Incidence in Manisa of Trichomonas vaginalis in women with a vaginal discharge.]. Turkiye Parazitol Derg 2005; 29: 7-9.
22. Ertabaklar H, Caner A, Döşkaya M, Demirtaş LO, Töz SO, Ertuğ S, et al. Trichomoniasis Tanısında Polimeraz Zincir Reaksiyonu ile Mikroskobi ve Kültür Yöntemlerinin Karşılaştırılması [Comparison of polymerase chain reaction with wet mount and culture methods for the diagnosis of trichomoniasis]. Turkiye Parazitol Derg 2011; 35: 1-5.
23. Huppert JS, Batteiger BE, Braslins P, Feldman JA, HobbsMM, Sankey HZ, et al. Use of an immunochromatographic assayforrapid detection of Trichomonas vaginalis in vaginal specimens. JClin Microbiol 2005; 43: 684-7.
24. Banneheke H, Fernandopulle R, Prathanapan S, de Silva G, FernandoN, Wickremasinghe R. Use of culture and immunochromatographictechnique for diagnosis of trichomoniasis in Sri Lanka. CeylonMedJ 2013; 58: 122-3.

Determination of Trichomonas vaginalis Frequency Among SymptomaticCases in Muğla Sıtkı Koçman Hospital Using Different Methods (Direct Microscopy, Culture, PCR and Immunochromatographic Method)

Yıl 2023, Cilt: 24 Sayı: 4, 321 - 326, 30.12.2023

Funda Sankur Sema Ertuğ , Erdoğan Malatyalı , Evren Tileklioğlu İbrahim Yıldız Hatice Ertabaklar

Öz

Objective: Trichomonas vaginalis (T. vaginalis) is a sexually transmitted protozoan parasite that colonizes the urogenital system. Because of the various health risks it poses, the diagnosis of the parasite is crucial. Therefore, the current study investigatedtheincidence of T. vaginalis in cases with vaginal discharge complaints.
Materials and Methods: A total of 150 cases presenting with vaginal discharge complaints were included in the study conductedat Muğla Sıtkı Koçman Hospital’s Gynaecology and Obstetrics Clinics. The demographic characteristics and clinical findingsof the cases were recorded. Swab samples obtained from the cases were evaluated for the detection of T. vaginalis using direct microscopy (DM), culture, polymerase chain reaction (PCR), and immunochromatographic rapid diagnostic test (RDT).
Results: Among the 150 women studied, two cases (1.3%) were positive for T. vaginalis with DM, whereas three cases (2%) werepositive using other methods, including culture, PCR, and RDT. There was no statistically significant difference between the methods(p>0.05). In addition to vaginal discharge, the most common symptoms observed were itching (45%) and abdominal pain (41%).
Conclusion: We reported the frequency of T. vaginalis in Muğla province. In addition, RDT was preferable for routine T. vaginalisdiagnosis because of its ease of use, lack of equipment requirements, and rapid results.

Anahtar Kelimeler

Trichomonas vaginalis, direct microscopy, culture, PCR, immunochromatographic technique

Kaynakça

1. Kissinger P. Trichomonas vaginalis: a review of epidemiologic, clinical and treatment issues. BMC Infect Dis 2015; 15: 307.
2. Thorburn AL. Alfred François Donné, 1801-1878, discoverer of Trichomonas vaginalis and of leukaemia. Br J Vener Dis 1974; 50: 377-80.
3. Rowley J, Vander Hoorn S, Korenromp E, Low N, Unemo M, AbuRaddad LJ, et al. Chlamydia, gonorrhoea, trichomoniasis and syphilis: global prevalence and incidence estimates, 2016. Bull World Health Organ 2019; 97: 548-62.
4. Menezes CB, Frasson AP, Tasca T. Trichomoniasis - are we givingthe deserved attention to the most common non-viral sexuallytransmitted disease worldwide? Microb Cell 2016; 3: 404-19.
5. Crucitti T, Jespers V, Mulenga C, Khondowe S, Vandepitte J, BuvéA. Non-sexual transmission of Trichomonas vaginalis in adolescent girls attending school in Ndola, Zambia. PLoS One 2011; 6: e16310.
6. Meites E, Gaydos CA, Hobbs MM, Kissinger P, Nyirjesy P, SchwebkeJR, et al. A Review of Evidence-Based Care of SymptomaticTrichomoniasis and Asymptomatic Trichomonas vaginalisInfections. Clin Infect Dis 2015; 61(Suppl 8): S837-48.
7. Değerli S, Şalk S, Malatyalı E. Sivas'ta vaginit ön tanılı hastalardaTrichomonas vaginalis sıklığı [Incidence in Sivas of Trichomonasvaginalis in patients with vaginitis]. Turkiye Parazitol Derg 2011; 35: 145-7.
8. Suay A, Yayla M, Mete Ö, Elçi S. 300 Hayat kadınında direkt mikroskobi ve kültür yöntemleriyle Trichomonas vaginalis vebunabağlı olarak Trikomoniyaz’ın araştırılması. Türkiye Parazitol Derg1995; 19: 170-3.
9. Hobbs MM, Seña AC. Modern diagnosis of Trichomonas vaginalisinfection. Sex Transm Infect 2013; 89: 434-8.
10. Fonseca THS, Oliveira FMS, Alacoque M, Rocha MI, LeiteHV, Santos JFG, et al. Immunocytochemistry Improving the Diagnosisof Trichomonas vaginalis Infections. Biomed Res Int 2017; 2017: 5642535.
11. Bhesania AH, Narayankhedkar A. Trichomoniasis-A Review. Int JCurr Microbiol App Sci 2016; 5: 731-41.
12. Lazenby GB. Trichomonas vaginalis screening and preventioninorderto impact the HIV pandemic: Isn't it time we take this infectionseriously? Infect Dis Rep 2011; 3: e4.
13. Fernando SD, Herath S, Rodrigo C, Rajapakse L. Clinical featuresand sociodemographic factors affecting Trichomonas vaginalisinfection in women attending a central sexually transmitteddiseases clinic in Sri Lanka. Indian J Sex TransmDis AIDS2012; 33: 25-31.
14. Garber GE. The laboratory diagnosis of Trichomonas vaginalis. CanJ Infect Dis Med Microbiol 2005; 16: 35-8.
15. Radonjic IV, Dzamic AM, Mitrovic SM, Arsic Arsenijevic VS, PopadicDM, Kranjcic Zec IF. Diagnosis of Trichomonas vaginalis infection: The sensitivities and specificities of microscopy, culture andPCRassay. Eur J Obstet Gynecol Reprod Biol 2006; 126: 116-20.
16. Morris SR, Bristow CC, Wierzbicki MR, Sarno M, Asbel L, FrenchA, et al. Performance of a single-use, rapid, point-of-care PCRdevicefor the detection of Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis: a cross-sectional study. Lancet Infect Dis 2021; 21: 668-76.
17. Madhivanan P, Li T, Trammell S, Desai C, Srinivas V, Arun A, et al. Performance of the OSOM Trichomonas Rapid Test for diagnosisof Trichomonas vaginalis infection among women in Mysore, India. Sex Health 2013; 10: 320-4.
18. Khatoon R, Jahan N, Ahmad S, Khan HM, Rabbani T. Comparisonof four diagnostic techniques for detection of Trichomonas vaginalisinfection in females attending tertiary care hospital of NorthIndia. Indian J Pathol Microbiol 2015; 58: 36-9.
19. Huppert JS, Batteiger BE, Braslins P, Feldman JA, HobbsMM, Sankey HZ, et al. Use of an immunochromatographic assayforrapid detection of Trichomonas vaginalis in vaginal specimens. JClin Microbiol 2005; 43: 684-7.
20. Aycan Kaya Ö, Benk-Şilfeler D, Özgür T, Keskin Kurt R, Hamamcı B, Sefil N, et al. Bir üniversite hastanesi jinekoloji polikliniğinebaşvuran kadınlarda parazitolojik ve sitolojik yöntemlerleTrichomonas vaginalis araştırılması. Konuralp Tıp Dergisi 2015; 7: 146-8.
21. Ostan I, Sözen U, Lımoncu ME, Kılımcıoğlu AA, Ozbılgın A. Manisa'da vajinal akıntılı kadınlarda Trichomonas vaginalis sıklığı [Incidence in Manisa of Trichomonas vaginalis in women with a vaginal discharge.]. Turkiye Parazitol Derg 2005; 29: 7-9.
22. Ertabaklar H, Caner A, Döşkaya M, Demirtaş LO, Töz SO, Ertuğ S, et al. Trichomoniasis Tanısında Polimeraz Zincir Reaksiyonu ile Mikroskobi ve Kültür Yöntemlerinin Karşılaştırılması [Comparison of polymerase chain reaction with wet mount and culture methods for the diagnosis of trichomoniasis]. Turkiye Parazitol Derg 2011; 35: 1-5.
23. Huppert JS, Batteiger BE, Braslins P, Feldman JA, HobbsMM, Sankey HZ, et al. Use of an immunochromatographic assayforrapid detection of Trichomonas vaginalis in vaginal specimens. JClin Microbiol 2005; 43: 684-7.
24. Banneheke H, Fernandopulle R, Prathanapan S, de Silva G, FernandoN, Wickremasinghe R. Use of culture and immunochromatographictechnique for diagnosis of trichomoniasis in Sri Lanka. CeylonMedJ 2013; 58: 122-3.

Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil	İngilizce
Konular	Klinik Mikrobiyoloji
Bölüm	Araştırma Makalesi
Yazarlar	Funda Sankur Sema Ertuğ Erdoğan Malatyalı Evren Tileklioğlu İbrahim Yıldız Hatice Ertabaklar
Yayımlanma Tarihi	30 Aralık 2023
Yayımlandığı Sayı	Yıl 2023 Cilt: 24 Sayı: 4

Kaynak Göster

EndNote	Sankur F, Ertuğ S, Malatyalı E, Tileklioğlu E, Yıldız İ, Ertabaklar H (01 Aralık 2023) Determination of Trichomonas vaginalis Frequency Among SymptomaticCases in Muğla Sıtkı Koçman Hospital Using Different Methods (Direct Microscopy, Culture, PCR and Immunochromatographic Method). Meandros Medical And Dental Journal 24 4 321–326.

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