Öz
Objective: This study aimed to establish in vivo adipose tissue, autologous transplantation of preadipocytes and adipocytes amplified in rat adipose culture suspensions and to compare histological features, adipose tissue formation and permanent volume following autologous transfer.
Materials and Methods: There were 32 rats in the study divided into 4 groups. Adipocyte cell suspensions were injected into the scapular and evaluated 4, 8, 12, and 24 weeks later for groups I, II, III, and IV, respectively. Histological and volumetric changes were detected.
Results: Although fatty tissue volume increased with time, there was no significant difference was between the groups. Histological scores were not different between groups (p>0.05).
Conclusion: In our study, the formation of fatty tissue in healthy appearance was observed in a similar manner after autologous transplantation of the preadipocytes and adipocyte cells in the culture. In the future, we believe that the preadipocyte cell suspension can be transplanted in cell culture and can be transferred autologous to fill the soft tissue defect in the same patient.
Anahtar Kelimeler
Adipocyte cell culture autologous tissue transfer pre-adipocyte cell culture
Kaynakça
- 1. Neuber GA. Fett Transplantation. Verl Dtsch Ges Chir 1893; 22: 66.
- 2. Billings E Jr, May JW Jr. Historical review and present status of free fat graft autotransplantation in plastic and reconstructive surgery. Plast Reconstr Surg 1989; 83: 368-81.
- 3. Lexer E. Freie fett transplantation. Dtsch MEd Wochenschr 1910; 36: 640.
- 4. Hinderer UT, del Rio JL. Erich Lexer’s mammaplasty. Aesthetic Plast Surg 1992; 16: 101-7.
- 5. Kaufman MR, Bradley JP, Dickinson B, Heller JB, Wasson K, O’Hara C, et al. Autologous fat transfer national consensus survey: trends in techniques for harvest, preparation, and application, and perception of short- and long-term results. Plast Reconstr Surg 2007; 119: 323-31.
- 6. Vanessa van H, Skurk T, Hauner H. Primary culture and differentiation of human adipocyte precursor cells. Human cell culture protocols. Humana Press 2005: 125-35.
- 7. Marler JJ, Guha A, Rowley J, Koka R, Mooney D, Upton J, et al. Soft-tissue augmentation with injectable alginate and syngeneic fibroblasts. Plast Reconstr Surg 2000; 105: 2049-58.
- 8. Torio-Padron N, Baerlecken N, Momeni A, Stark GB, Borges J. Engineering of adipose tissue by injection of human preadipocytes in fibrin. Aesthetic Plast Surg 2007; 31: 285-93.
- 9. Cho SW, Kim I, Kim SH, Rhie JW, Choi CY, Kim BS. Enhancement of adipose tissue formation by implantation of adipogenic-differentiated preadipocytes. Biochem Biophys Res Commun 2006; 345: 588-94.
- 10. Hemmrich K, von HD, Rendchen R, Di BC, Milella E, Pallua N. Implantation of preadipocyte-loaded hyaluronic acid-based scaffolds into nude mice to evaluate potential for soft tissue engineering. Biomaterials 2005; 26: 7025-37.
- 11. Yoshimura K, Suga H, Eto H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen Med 2009; 4: 265-73.
Öz
Kaynakça
- 1. Neuber GA. Fett Transplantation. Verl Dtsch Ges Chir 1893; 22: 66.
- 2. Billings E Jr, May JW Jr. Historical review and present status of free fat graft autotransplantation in plastic and reconstructive surgery. Plast Reconstr Surg 1989; 83: 368-81.
- 3. Lexer E. Freie fett transplantation. Dtsch MEd Wochenschr 1910; 36: 640.
- 4. Hinderer UT, del Rio JL. Erich Lexer’s mammaplasty. Aesthetic Plast Surg 1992; 16: 101-7.
- 5. Kaufman MR, Bradley JP, Dickinson B, Heller JB, Wasson K, O’Hara C, et al. Autologous fat transfer national consensus survey: trends in techniques for harvest, preparation, and application, and perception of short- and long-term results. Plast Reconstr Surg 2007; 119: 323-31.
- 6. Vanessa van H, Skurk T, Hauner H. Primary culture and differentiation of human adipocyte precursor cells. Human cell culture protocols. Humana Press 2005: 125-35.
- 7. Marler JJ, Guha A, Rowley J, Koka R, Mooney D, Upton J, et al. Soft-tissue augmentation with injectable alginate and syngeneic fibroblasts. Plast Reconstr Surg 2000; 105: 2049-58.
- 8. Torio-Padron N, Baerlecken N, Momeni A, Stark GB, Borges J. Engineering of adipose tissue by injection of human preadipocytes in fibrin. Aesthetic Plast Surg 2007; 31: 285-93.
- 9. Cho SW, Kim I, Kim SH, Rhie JW, Choi CY, Kim BS. Enhancement of adipose tissue formation by implantation of adipogenic-differentiated preadipocytes. Biochem Biophys Res Commun 2006; 345: 588-94.
- 10. Hemmrich K, von HD, Rendchen R, Di BC, Milella E, Pallua N. Implantation of preadipocyte-loaded hyaluronic acid-based scaffolds into nude mice to evaluate potential for soft tissue engineering. Biomaterials 2005; 26: 7025-37.
- 11. Yoshimura K, Suga H, Eto H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen Med 2009; 4: 265-73.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Plastik, Rekonstrüktif ve Estetik Cerrahi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Haziran 2022 |
| Yayımlandığı Sayı | Yıl 2022 Cilt: 23 Sayı: 2 |