Araştırma Makalesi
Axinella damicornis süngerinden izole edilen sucul bakterilerin proteaz üretkenliklerinin araştırılması ve üretilen proteaz enziminin kısmi karakterizasyonu
Öz
Proteolitik enzim grubundan proteazlar ticari enzim pazarında önemli bir yere sahiptirler. Kullanım alanlarına göre farklı karakteristik özelliklere sahip olmaları istenilen proteazların üstün özellikte olanlarının endüstriye kazandırılması önem teşkil etmektedir. Bu yüzden yeni mikrobiyal türlerin izolasyonu ve bu türlerden üretimlerin yapılması önemli bir çalışma konusudur. Bu çalışmada Kaş, Antalya, Türkiye’den izole edilen 81 sucul bakteriyel izolatın proteaz üretkenlikleri taranmıştır. En üretken izolatın Microbacterium genusuna ait olduğu belirlenmiş ve bu izolat ile çalkalamalı flask üretimlerde 166 U ml-1 aktivitesinde proteaz üretilmiştir. Ayrıca üretilen proteazın optimum sıcaklık ve pH değerlerinin 35oC ve pH 8 olduğu belirlenmiştir. Bunun yanında enzim aktivitesinin 10 mM MnCl2 etkisinde arttığı ve enzimin uzun süreli depolanmasında 4oC’nin -20oC ve 25oC koşullarına göre daha avantajlı olduğu gösterilmiştir.
Anahtar Kelimeler
Kaynakça
- Blommel PG, Becker KJ, Duvnjak P, Fo BG (2007) Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition. Biotechnology Progress 23(3): 585-598.
- Cao EH, Chen YH, Cui ZF, Foster PR (2003) Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions. Biotechnology and Bioengineering 82(6): 684-690.
- Cui HX, Wang LP, Yu Y (2015) Production and characterization of alkaline protease from a high yielding and moderately halophilic strain of SD11 marine bacteria. Journal of Chemistry Doi: 10.1155/2015/798304.
- Fulzele R, DeSa E, Yadav A, Shouche Y, Bhadekar R (2011) Characterization of novel extracellular protease produced by marine bacterial isolate from the Indian Ocean. Brazilian Journal of Microbiology 42(4): 1364-1373.
- Gessesse A, Gashe BA (1997) Production of alkaline protease by an alkaliphilic bacteria isolated from an alkaline soda lake. Biotechnology Letters 19(5): 479-481.
- Kannikan V, Arumugam P, Rebecca J (2018) Optimization of protease enzyme production by marine actinomycetes. International Journal of Pharma and Bio Sciences 8(3): 188-194.
- Manachini PL, Fortina MG, Parini C (1988) Thermostable alkaline protease produced by Bacillus thermoruber - a new species of Bacillus. Applied Microbiology and Biotechnology 28(4): 409-413.
- Pilanee V, Taweesiri M, Waraporn A (2008) Silk degumming solution as substrate for microbial protease production. Journal of Biotechnology 136(3): 543-551.
- Rahman RNZA, Razak CN, Ampon K, Basri M, Zin WM, Yunus W, Salleh AB (1994) Purification and characterization of a heat-stable alkaline protease from Bacillus stearothermophilus F1. Applied Microbiology and Biotechnology 40(6): 822-827.
- Razzaq A, Shamsi S, Ali A, Ali Q, Sajjad M, Malik A, Ashraf M (2019) Microbial proteases applications. Frontiers in Bioengineering and Biotechnology 7: 110.
- Saggu SK, Jha G, Mishra PC (2019) Enzymatic degradation of biofilm by metalloprotease from Microbacterium sp. SKS10. Frontiers in Bioengineering and Biotechnology 7: 192.
- Sharma KM, Kumar R, Panwar S, Kumar A (2017) Microbial alkaline proteases: Optimization of production parameters and their properties. Journal of Genetic Engineering and Biotechnology 15(1): 115-126.
- Thys RCS, Brandelli A (2006) Purification and properties of a keratinolytic metalloprotease from Microbacterium sp. Journal of Applied Microbiology 101(6): 1259-1268.
- Ul Qader SA, Sattar H, Aman A (2017) Effect of metal ions, solvents and surfactants on the activity of protease from Aspergillus niger KIBGE-IB36. Journal of Basic & Applied Sciences 13: 491-495.
- Zhang XY, Han XX, Chen XL, Dang HY, Xie BB, Qin QL, Shi M, Zhou BC, Zhang YZ (2015) Diversity of cultivable protease-producing bacteria in sediments of Jiaozhou Bay, China. Frontiers in Microbiology 6: 1021.
Investigation of protease productivity of marine bacteria isolated from Axinella damicornis sponge and partial characterization of produced protease
Öz
Proteases from the proteolytic enzyme group have an important position in the commercial enzyme market. It is crucial to bring the superior properties of the proteases, which are desired to have different characteristics according to their usage, to the industry. Therefore, isolation of new microbial species and enzyme productions from those is a critical study subject. In this study, protease productivity of the 81 bacteria were screened which were isolated from Kas, Antalya, Turkey. It was determined that the most productive strain belongs to the Microbacterium genus, and this strain produced 166 U ml-1 protease activity in shaking flask productions. In addition, the optimum temperature and pH values of the produced protease were determined to be 35oC and pH 8. Also, protease enzyme activity increase under the influence of 10 mM MnCl2 and 4oC condition is more advantageous than of -20oC and 25oC in long-term storage of the enzyme.
Anahtar Kelimeler
Protease Marine bacteria Enzyme production Enzyme characterization
Kaynakça
- Blommel PG, Becker KJ, Duvnjak P, Fo BG (2007) Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition. Biotechnology Progress 23(3): 585-598.
- Cao EH, Chen YH, Cui ZF, Foster PR (2003) Effect of freezing and thawing rates on denaturation of proteins in aqueous solutions. Biotechnology and Bioengineering 82(6): 684-690.
- Cui HX, Wang LP, Yu Y (2015) Production and characterization of alkaline protease from a high yielding and moderately halophilic strain of SD11 marine bacteria. Journal of Chemistry Doi: 10.1155/2015/798304.
- Fulzele R, DeSa E, Yadav A, Shouche Y, Bhadekar R (2011) Characterization of novel extracellular protease produced by marine bacterial isolate from the Indian Ocean. Brazilian Journal of Microbiology 42(4): 1364-1373.
- Gessesse A, Gashe BA (1997) Production of alkaline protease by an alkaliphilic bacteria isolated from an alkaline soda lake. Biotechnology Letters 19(5): 479-481.
- Kannikan V, Arumugam P, Rebecca J (2018) Optimization of protease enzyme production by marine actinomycetes. International Journal of Pharma and Bio Sciences 8(3): 188-194.
- Manachini PL, Fortina MG, Parini C (1988) Thermostable alkaline protease produced by Bacillus thermoruber - a new species of Bacillus. Applied Microbiology and Biotechnology 28(4): 409-413.
- Pilanee V, Taweesiri M, Waraporn A (2008) Silk degumming solution as substrate for microbial protease production. Journal of Biotechnology 136(3): 543-551.
- Rahman RNZA, Razak CN, Ampon K, Basri M, Zin WM, Yunus W, Salleh AB (1994) Purification and characterization of a heat-stable alkaline protease from Bacillus stearothermophilus F1. Applied Microbiology and Biotechnology 40(6): 822-827.
- Razzaq A, Shamsi S, Ali A, Ali Q, Sajjad M, Malik A, Ashraf M (2019) Microbial proteases applications. Frontiers in Bioengineering and Biotechnology 7: 110.
- Saggu SK, Jha G, Mishra PC (2019) Enzymatic degradation of biofilm by metalloprotease from Microbacterium sp. SKS10. Frontiers in Bioengineering and Biotechnology 7: 192.
- Sharma KM, Kumar R, Panwar S, Kumar A (2017) Microbial alkaline proteases: Optimization of production parameters and their properties. Journal of Genetic Engineering and Biotechnology 15(1): 115-126.
- Thys RCS, Brandelli A (2006) Purification and properties of a keratinolytic metalloprotease from Microbacterium sp. Journal of Applied Microbiology 101(6): 1259-1268.
- Ul Qader SA, Sattar H, Aman A (2017) Effect of metal ions, solvents and surfactants on the activity of protease from Aspergillus niger KIBGE-IB36. Journal of Basic & Applied Sciences 13: 491-495.
- Zhang XY, Han XX, Chen XL, Dang HY, Xie BB, Qin QL, Shi M, Zhou BC, Zhang YZ (2015) Diversity of cultivable protease-producing bacteria in sediments of Jiaozhou Bay, China. Frontiers in Microbiology 6: 1021.
Toplam 15 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Ziraat Mühendisliği |
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Ağustos 2020 |
| Gönderilme Tarihi | 14 Şubat 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 33 Sayı: 2 |
Kaynak Göster
Cited By
Production, Optimization and Partial Characterization of Alkaline Protease from Bacillus subtilis spp. subtilis NRRL B-3384 and B-3387
Hittite Journal of Science and Engineering
https://doi.org/10.17350/HJSE19030000300
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