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Determination of Effective Mutation Dose (ED50) To Be Used In Variety Treatment In Tea Plant

Yıl 2025, Cilt: 6 Sayı: 2, 569 - 579, 29.07.2025

Burcu Göksu Karaoğlu , Yıldız Aka Kaçar , Kadriye Yaprak Kantoglu , Burak Kunter , Keziban Yazıcı

https://doi.org/10.53501/rteufemud.1543862

Öz

Although tea is cultivated only in the Eastern Black Sea region of Türkiye, all tea plantations in the area have been established from seeds since the beginning of tea farming. This has negatively affected both yield and quality. The high level of heterozygosity in tea plants and the presence of self-incompatibility mechanisms prolong breeding efforts. Mutation breeding is used as a fast and practical method to induce genetic variation. In leading tea-producing countries such as China, India, Kenya, and Sri Lanka, commercially important tea cultivars have been developed through mutation breeding. However, no such studies have been conducted in Türkiye. This study aimed to determine the effective mutation dose that can be used to induce variation in tea plants. The research was conducted between 2020 and 2021 at the Tea Research Greenhouse of the Faculty of Agriculture, Recep Tayyip Erdoğan University. The plant material used was 3–4-node shoots of the Zihni Derin tea cultivar. These shoots were irradiated with 0, 10, 20, 30, 40, 50, 60, and 70 Gray (Gy) doses using the “Ob-Servo Sanguis Co-60 Research Irradiator” gamma irradiation device at the Turkish Energy, Nuclear and Mineral Research Agency. Single-node cuttings obtained from the irradiated shoots were planted in a peat-vermiculite mixture. Survival and rooting rates of the tea cuttings exposed to different gamma doses were recorded, and shoot length (mm) was measured in the growing tea seedlings. Survival and rooting rates decreased with increasing radiation dose. Based on a probit regression analysis of shoot length, the “Effective Mutation Dose” for tea was determined to be 11.45 Gy. This study represents the first mutation breeding research on tea in Türkiye and is expected to provide a foundation for future work in this field.

Anahtar Kelimeler

Mutation Breeding Tea Effective Dose Gamma ray Variety Development

Kaynakça

  • Ahloowalia, B.S., Maluszynski, M., Nichterlein, K. (2004). Global impact of mutation-derived varieties. Euphytica, 135, 187-204. https://doi.org/10.1023/B:EUPH.0000014914.85465.4f.
  • Ali, M.A., Samad, M.A., Amin, M.K. (2005). Effect of gamma rays on the growth performance of Bangladesh clone tea. Korean Journal of Environmental Agriculture, 24(1), 66-70. https://doi.org/10.5338/KJEA.2005.24.1.66.
  • Alyanak, N. (2019). Possibilities to Generate Mutation Using Co60 in Isabella and Alphonse Lavallèe Grape Cultivars, Master's thesis, Ordu University, Türkiye.
  • Ayar., A. (2022). Fig (Ficus carica L.) field gene banks and studies. Biological Diversity and Conservation, 15(3), 308-319. https://doi.org/ 10.46309/biodicon.2022.1144871
  • Babu, S., (2008). Studies on ınduced mutation and fixing of LD50 value in tea (Camellia spp. (L.)). Journal of Plantation Crops, 36(3), 200-203. Acces link: https://www.cabidigitallibrary.org/doi/full/10.5555/20103048543
  • Banerjee, B. (1992). Selection and breeding of tea. In: Tea: cultivation to consumption, (Willson, K.C., Clifford, M.N.), Springer, ISBN No: 978-94-010-5027-2, Dordrecht, XX, 769. https://doi.org/10.1007/978-94-011-2326-6_3
  • Chan, Y.K. (2009). Radiation-induced mutation breeding of papaya. Induced Mutation in Tropical Fruit Trees. Vienna, Austria, IAEA-TECDOC-1615, ISBN: 978–92–0–102709–2, ISSN: 1011–4289, p: 161.
  • Chen, L., Zhou, Z.X., Yang, Y.J. (2007). Genetic improvement and breeding of tea plant (Camellia sinensis) in China: from individual selection to hybridization and molecular breeding. Euphytica, 154, 239-248. https://doi.org/10.1007/s10681-006-9292-3.
  • Çimen, B., Yeşiloğlu, T., İncesu, M., Yılmaz, B., Tuzcu, Ö. (2019). Evaluation of Robinson Mutant Population Derived By Gamma Irradiation. Ege Üniversitesi Ziraat Fakültesi Dergisi, 56(3), 375-381. https://doi.org/10.20289/zfdergi.526322
  • Dong, L.J., Gong, J.W., Wang, P.F. (1985). A preliminary report on the sensitivity of tea plants to ırradiation. Applicaiton of Atomic Energy in Agriculture (YuanzinengNongyeYingyong), 1, 51-55.
  • Food and Agriculture Organization (FAO). (2021). Tea Production Quantity. https://www.fao.org/faostat/en/#data/QCL, 22.03.2023
  • Gunasekare, M.T.K., (2007). Applications of molecular markers to the genetic improvement of Camellia sinensis L. (tea). A review. The Journal of Horticultural Science and Biotechnology, 82 (2), 161-169. https://doi.org/ 10.1080/14620316.2007.11512214.
  • Hasim, A.A., Shamsiah, A., Hussein, S. (2021). Induced mutations using gamma-ray and multiplication of plantlet through micro cross section culture of banana (Musa acuminata cv. Berangan). International Journal of Recent Research in Life Sciences,1(3), 22-28. https://doi.org/10.1088/1755-1315/757/1/012007
  • International Atomic Energy Agency (IAEA), (2009). International Atomic Energy Agency, Plant Breeding and Genetics Section. Vienna, Austria, IAEA-TECDOC--1615, 170s.. External URL: http://www.pub.iaea.org/MTCD/Publications/PDF/TE_1615_web.pdf, http://www.iaea.org/books, Date of Access: 05.10.2024
  • Kamau, P., Maritim, T., Chalo, R., Kamunya, S., Wanyoko, J. (2014). Gamma irradiation induces changes in biochemical properties of tea. Tea, 35(1), 24-32.
  • Kamunya, S.M., Wachira, F.N., Pathak, R.S., Muoki, R.C., Sharma, R.K. (2012). Tea Improvement in Kenya. Global tea breeding: achievements, challenges and perspectives, Springer, ISBN No: 978-3-642-31878-8, Springer Berlin, Heidelberg, XX, 384. https://doi.org/10.1007/978-3-642-31878-8
  • Karmarkar V.M., Kulkarni V.M., Suprasanna P., Bapat V.A., Rao P.S. (2001). Radio-sensitivity of in vivo and in vitro cultures of banana cv Basrai (AAA). Fruits, 56, 67-74. https://doi.org/10.1051/fruits:2001114
  • Khan, A.Q. (2012). Tea Germplasm and Improvement in Bangladesh. Global tea breeding: achievements, challenges and perspectives, Springer, ISBN No: 978-3-642-31878-8, Springer Berlin, Heidelberg, XX, 384. https://doi.org/10.1007/978-3-642-31878-8
  • Kudo, K., Futsuhara, Y. (1974). Effects of r-ray irradiation on the development of leaf and flower buds in tea plants. Japanese Journal of Breeding, 24(4),169-175.
  • Kunter, B., Baş, M., Özyiğit, S., Kantoğlu, Y., Burak, M. (2013). Improving New Traits By Mutation Breeding in Sweet Cherry, International Plant Breeding Congress, 10-14 November 2013, Antalya, Türkiye.
  • Ma, J.Q., Yao, M.Z., Ma, C.L., Wang, X.C., Jin, J.Q., Wang, X.M., Chen, L. (2014). Construction of a SSR-based genetic map and identification of QTLs for catechins content in tea plant (Camellia sinensis). PloS one, 9(3), e93131. https://doi.org/10.1371/journal.pone.0093131
  • Marasalı, B., Kunter, B., Değirmenci, D., Keskin, N., Kantoğlu, K.Y., Çelik, H., Tutluer, İ., Uslu, N., Sağel, Z., Peşkircioğlu, H. (2003). Determination of effective mutation doses and first effects on M1V1 generation in native grape cultivars. IV. National Horticulture Congress, 8-12 September 2003, Antalya, Türkiye.
  • Marcu, D., Damian, G., Cosma, C., Cristea, V. (2013). Dose-dependent effects of gamma radiation on lettuce (Lactuca sativa var. Capitata) seedlings. International Journal of Radiation Biology, 89(3), 219-223. https://doi.org/10.3109/09553002.2013.734946
  • Mikautadze, N.G. (1986). Production of polyploid forms of tea following gamma irradiation of seeds. Materialy 5 S’’ezda Gruzinskogo obshchestva genetikov i selektsionerov, pp: 100-106.
  • Muhammad, I., Rafii, M.Y., Nazli, M.H., Ramlee, S.I., Harun, A.R., Oladosu, Y. (2021). Determination of lethal (LD) and growth reduction (GR) doses on acute and chronic gamma-irradiated Bambara groundnut (Vigna subterranea (L.) Verdc.) varieties. Journal of Radiation Research and Applied Sciences, 14(1), 133-145. https://doi.org/10.1080/16878507.2021.1883320
  • Nakagawa, H. (2009). Induced mutations in plant breeding and biological researches in Japan. In: Induced Plant Mutations in the Genomics, Era, Shu QY (ed), Food and Agriculture Organization of the United Nations, ISBN: 978-92-5-106324-8, Italy, 458.
  • Nakayama, A. (1973). Induction of the somatic mutations in tea plants by gamma irradiation. Gamma Field Symposia, 12, 37-47.
  • Nakayama, A. (1976). Somatic mutation of tea plants ınduced by gamma ırradiation. Japan Agricultural Research Quarterly,10(2), 79-83.
  • Nwachukwu, E.C., Mbanaso, E.N.A., Nwosu, K.I. (2009). The development of new genotypes of the white yam by mutation induction using yam mini-tubers. In: Induced Plant Mutations in the Genomics, Era, Shu QY (ed), Food and Agriculture Organization of the United Nations, ISBN: 978-92-5-106324-8, Italy, 458.
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  • Özyiğit, S., Baş, M., Doğan, A., Şen, A. (2021). Determination of cracking conditions of cherry varieties/types obtaıned by hybridization and mutation breeding. Fruit Science, 8(2), 34-45. https://doi.org/10.51532/meyve.1029788
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Çay Bitkisinin Çeşit Islahında Kullanılacak Etkili Mutasyon Dozunun (ED50) Belirlenmesi

Yıl 2025, Cilt: 6 Sayı: 2, 569 - 579, 29.07.2025

Burcu Göksu Karaoğlu , Yıldız Aka Kaçar , Kadriye Yaprak Kantoglu , Burak Kunter , Keziban Yazıcı

https://doi.org/10.53501/rteufemud.1543862

Öz

Çay bitkisi Türkiye’de sadece Doğu Karadeniz bölgesinde yetiştirilmekle birlikte yetiştiriciliğinin bu bölgede yaygınlaşmaya başladığı tarihten itibaren bahçeler tohumla tesis edilmiştir. Bu durum verim ve kaliteyi olumsuz yönde etkilemektedir. Çay bitkisinde heterezigoti oranının yüksek olması ve kendine uyuşmazlık mekanizmasının varlığı ıslah çalışmalarının süresini uzatmaktadır. Hızlı ve pratik bir şekilde genetik çeşitlilik ortaya çıkarmak amacı ile mutasyon ıslahı yöntemi kullanılmaktadır. Çay üretim sıralamasında ilk sıralarda yer alan Çin, Hindistan, Kenya ve Sri Lanka gibi ülkelerde mutasyon ıslahı ile ticarete konu olan önemli çay çeşitleri geliştirilmiştir. Ülkemizde ise bu konuda bir çalışma yapılmamıştır. Bu çalışmada, çay bitkisinde varyasyon oluşturmada kullanılabilecek etkili mutasyon dozununun belirlenmesi amaçlanmıştır. Çalışmamız Recep Tayyip Erdoğan Üniversitesi, Ziraat Fakültesi, Çay Araştırma Serasında 2020-2021 yıllarında yürütülmüştür. Materyal olarak kullanılan Zihni Derin çay çeşidine ait 3-4 gözlü sürgünlere Türkiye Enerji Nükleer ve Maden Araştırma Kurumu’nda İzotop marka “Ob-Servo Sanguis Co-60 Research Irradiator” model gama ışınlama cihazı ile 0, 10, 20, 30, 40, 50, 60 ve 70 Gray (Gy) dozlarında ışınlama yapılarak tek gözlü olarak alınan çay çelikleri torf-vermikulit karışımına dikilmiştir. Farklı dozlarda gama ışını uygulanan çay çeliklerinin canlılık ve köklenme oranları belirlenmiş büyüyen çay fidanlarında ise sürgün uzunluğu (mm) ölçümleri yapılmıştır. Çay çeliklerinin canlılık ve köklenme oranları doz artışına bağlı olarak azalma göstermiştir. Sürgün uzunluğuna göre yapılan probit regresyon analizi sonucunda ise çayda ‘’Etkili Mutasyon Dozunun’’ 11, 45 Gy olduğu tespit edilmiştir. Bu çalışma Türkiye’de çayda mutasyon ıslahı konusunda yapılan ilk çalışma olup bundan sonraki çalışmalara ışık tutacak niteliğe sahiptir.

Anahtar Kelimeler

Mutasyon Islahı Çay Etkili Doz Gama Işını

Kaynakça

  • Ahloowalia, B.S., Maluszynski, M., Nichterlein, K. (2004). Global impact of mutation-derived varieties. Euphytica, 135, 187-204. https://doi.org/10.1023/B:EUPH.0000014914.85465.4f.
  • Ali, M.A., Samad, M.A., Amin, M.K. (2005). Effect of gamma rays on the growth performance of Bangladesh clone tea. Korean Journal of Environmental Agriculture, 24(1), 66-70. https://doi.org/10.5338/KJEA.2005.24.1.66.
  • Alyanak, N. (2019). Possibilities to Generate Mutation Using Co60 in Isabella and Alphonse Lavallèe Grape Cultivars, Master's thesis, Ordu University, Türkiye.
  • Ayar., A. (2022). Fig (Ficus carica L.) field gene banks and studies. Biological Diversity and Conservation, 15(3), 308-319. https://doi.org/ 10.46309/biodicon.2022.1144871
  • Babu, S., (2008). Studies on ınduced mutation and fixing of LD50 value in tea (Camellia spp. (L.)). Journal of Plantation Crops, 36(3), 200-203. Acces link: https://www.cabidigitallibrary.org/doi/full/10.5555/20103048543
  • Banerjee, B. (1992). Selection and breeding of tea. In: Tea: cultivation to consumption, (Willson, K.C., Clifford, M.N.), Springer, ISBN No: 978-94-010-5027-2, Dordrecht, XX, 769. https://doi.org/10.1007/978-94-011-2326-6_3
  • Chan, Y.K. (2009). Radiation-induced mutation breeding of papaya. Induced Mutation in Tropical Fruit Trees. Vienna, Austria, IAEA-TECDOC-1615, ISBN: 978–92–0–102709–2, ISSN: 1011–4289, p: 161.
  • Chen, L., Zhou, Z.X., Yang, Y.J. (2007). Genetic improvement and breeding of tea plant (Camellia sinensis) in China: from individual selection to hybridization and molecular breeding. Euphytica, 154, 239-248. https://doi.org/10.1007/s10681-006-9292-3.
  • Çimen, B., Yeşiloğlu, T., İncesu, M., Yılmaz, B., Tuzcu, Ö. (2019). Evaluation of Robinson Mutant Population Derived By Gamma Irradiation. Ege Üniversitesi Ziraat Fakültesi Dergisi, 56(3), 375-381. https://doi.org/10.20289/zfdergi.526322
  • Dong, L.J., Gong, J.W., Wang, P.F. (1985). A preliminary report on the sensitivity of tea plants to ırradiation. Applicaiton of Atomic Energy in Agriculture (YuanzinengNongyeYingyong), 1, 51-55.
  • Food and Agriculture Organization (FAO). (2021). Tea Production Quantity. https://www.fao.org/faostat/en/#data/QCL, 22.03.2023
  • Gunasekare, M.T.K., (2007). Applications of molecular markers to the genetic improvement of Camellia sinensis L. (tea). A review. The Journal of Horticultural Science and Biotechnology, 82 (2), 161-169. https://doi.org/ 10.1080/14620316.2007.11512214.
  • Hasim, A.A., Shamsiah, A., Hussein, S. (2021). Induced mutations using gamma-ray and multiplication of plantlet through micro cross section culture of banana (Musa acuminata cv. Berangan). International Journal of Recent Research in Life Sciences,1(3), 22-28. https://doi.org/10.1088/1755-1315/757/1/012007
  • International Atomic Energy Agency (IAEA), (2009). International Atomic Energy Agency, Plant Breeding and Genetics Section. Vienna, Austria, IAEA-TECDOC--1615, 170s.. External URL: http://www.pub.iaea.org/MTCD/Publications/PDF/TE_1615_web.pdf, http://www.iaea.org/books, Date of Access: 05.10.2024
  • Kamau, P., Maritim, T., Chalo, R., Kamunya, S., Wanyoko, J. (2014). Gamma irradiation induces changes in biochemical properties of tea. Tea, 35(1), 24-32.
  • Kamunya, S.M., Wachira, F.N., Pathak, R.S., Muoki, R.C., Sharma, R.K. (2012). Tea Improvement in Kenya. Global tea breeding: achievements, challenges and perspectives, Springer, ISBN No: 978-3-642-31878-8, Springer Berlin, Heidelberg, XX, 384. https://doi.org/10.1007/978-3-642-31878-8
  • Karmarkar V.M., Kulkarni V.M., Suprasanna P., Bapat V.A., Rao P.S. (2001). Radio-sensitivity of in vivo and in vitro cultures of banana cv Basrai (AAA). Fruits, 56, 67-74. https://doi.org/10.1051/fruits:2001114
  • Khan, A.Q. (2012). Tea Germplasm and Improvement in Bangladesh. Global tea breeding: achievements, challenges and perspectives, Springer, ISBN No: 978-3-642-31878-8, Springer Berlin, Heidelberg, XX, 384. https://doi.org/10.1007/978-3-642-31878-8
  • Kudo, K., Futsuhara, Y. (1974). Effects of r-ray irradiation on the development of leaf and flower buds in tea plants. Japanese Journal of Breeding, 24(4),169-175.
  • Kunter, B., Baş, M., Özyiğit, S., Kantoğlu, Y., Burak, M. (2013). Improving New Traits By Mutation Breeding in Sweet Cherry, International Plant Breeding Congress, 10-14 November 2013, Antalya, Türkiye.
  • Ma, J.Q., Yao, M.Z., Ma, C.L., Wang, X.C., Jin, J.Q., Wang, X.M., Chen, L. (2014). Construction of a SSR-based genetic map and identification of QTLs for catechins content in tea plant (Camellia sinensis). PloS one, 9(3), e93131. https://doi.org/10.1371/journal.pone.0093131
  • Marasalı, B., Kunter, B., Değirmenci, D., Keskin, N., Kantoğlu, K.Y., Çelik, H., Tutluer, İ., Uslu, N., Sağel, Z., Peşkircioğlu, H. (2003). Determination of effective mutation doses and first effects on M1V1 generation in native grape cultivars. IV. National Horticulture Congress, 8-12 September 2003, Antalya, Türkiye.
  • Marcu, D., Damian, G., Cosma, C., Cristea, V. (2013). Dose-dependent effects of gamma radiation on lettuce (Lactuca sativa var. Capitata) seedlings. International Journal of Radiation Biology, 89(3), 219-223. https://doi.org/10.3109/09553002.2013.734946
  • Mikautadze, N.G. (1986). Production of polyploid forms of tea following gamma irradiation of seeds. Materialy 5 S’’ezda Gruzinskogo obshchestva genetikov i selektsionerov, pp: 100-106.
  • Muhammad, I., Rafii, M.Y., Nazli, M.H., Ramlee, S.I., Harun, A.R., Oladosu, Y. (2021). Determination of lethal (LD) and growth reduction (GR) doses on acute and chronic gamma-irradiated Bambara groundnut (Vigna subterranea (L.) Verdc.) varieties. Journal of Radiation Research and Applied Sciences, 14(1), 133-145. https://doi.org/10.1080/16878507.2021.1883320
  • Nakagawa, H. (2009). Induced mutations in plant breeding and biological researches in Japan. In: Induced Plant Mutations in the Genomics, Era, Shu QY (ed), Food and Agriculture Organization of the United Nations, ISBN: 978-92-5-106324-8, Italy, 458.
  • Nakayama, A. (1973). Induction of the somatic mutations in tea plants by gamma irradiation. Gamma Field Symposia, 12, 37-47.
  • Nakayama, A. (1976). Somatic mutation of tea plants ınduced by gamma ırradiation. Japan Agricultural Research Quarterly,10(2), 79-83.
  • Nwachukwu, E.C., Mbanaso, E.N.A., Nwosu, K.I. (2009). The development of new genotypes of the white yam by mutation induction using yam mini-tubers. In: Induced Plant Mutations in the Genomics, Era, Shu QY (ed), Food and Agriculture Organization of the United Nations, ISBN: 978-92-5-106324-8, Italy, 458.
  • Özen, M., Kocataş, H., Çobanoğlu, F., Ertan, B., Kösoğlu, İ., Tan, N., Gülşen, O. (2015). Mutation breeding studies on fig. Acta Horticulture, 1173, 93-98. https://doi.org/10.17660/ActaHortic.2017.1173.16
  • Özyiğit, S., Baş, M., Doğan, A., Şen, A. (2021). Determination of cracking conditions of cherry varieties/types obtaıned by hybridization and mutation breeding. Fruit Science, 8(2), 34-45. https://doi.org/10.51532/meyve.1029788
  • Rana, M.A., Usman, M., Fatima, B., Fatima, A., Rana, I.A., Rehman, W., Shoukat, D. (2020). Prospects of mutation breeding in grapefruit (Citrus paradisi Macf.). Journal of Horticultural Science and Technology, 3(2), 31-35. https://doi.org/10.46653/jhst20030231
  • Rashid, A., Alam, A.F.M.B. (1976). Effect of low and high doses of gamma radiation on tea cuttings and seeds. Tea Journal of Bangladesh, 12(1),18-22.
  • Rostini, N., Haeruman, M.K., Mansyah, E., Muas, I. (2003). Current status on mangosteen mutation breeding in Indonesia. Technological and Institutional Innovations for Sustainable Rural Development, October 8-10, 2003, Göttingen, Germany.
  • Sadhukhan, R., Swathi, K., Sarmah, D., Mandal, T. (2015). Effect of different doses of gamma rays on survivability and rooting ability in chrysanthemum (Chrysanthemum morifolim Ramat.). Journal. Crop Weed, 11, 62-65.
  • Sanada, T. (1988). Induced mutation breeding in fruit trees. In Gamma field symposia, 10(20), 87-108. https://inis.iaea.org/search/search.aspx?orig_q=RN:20033607
  • Sarathchandra T.M, Pieris R. (2001). Induction of mutations in tea (Camellia sinensis L.). Sri Lanka Association of the Advancement of Science, Proceeding of the 57th Annual Session of, Colombo.
  • Sarıçam, Ş., Kantoğlu, K.Y., Ellialtıoğlu, Ş.Ş. (2018). Determination of effective mutation dose for coated and uncoated lettuce (Lactuca sativa var. Longifolia cv. Cervantes) seeds. Tralleis Journal of Agricultural Sciences, 3(2), 229-242.
  • Shehasen, M.Z. (2019). Tea plant (Camellia sinensis) breeding mechanisms role in genetic improvement and production of major producing countries. International Journal of Research Studies in Science, Engineering and Technology, 6(11), 10-20.
  • Singh, I.D. (1980). Nonconventional approaches to the breeding of tea in north-east India. Two and a Bud, 27, 3–6.
  • Singh, N., Rawal, H.C., Angadi, U.B., Sharma, T.R., Singh, N.K., Mondal, T.K. (2022). A first-generation haplotype map (HapMap-1) of tea (Camellia sinensis L. O. Kuntz). Bioinformatics, 38(2), 318-324. https://doi.org/10.1093/bioinformatics/btab690.
  • Sukirtharuban, S., Ranaweera, K.K., Ranatunga, M.A.B., Perera, C. (2020). A short review on the application of mutation breeding techniques using physical mutagen on tea (Camellia sinensis (L.) O. Kuntze): An economically important medicinal herb. 1st International Conference and Exhibition on Siddha Medicine 2020, Srilanka.
  • Tan, L. Q., Peng, M., Xu, L. Y., Wang, L. Y., Chen, S. X., Zou, Y., Qi, N.G., Cheng, H. (2015). Fingerprinting 128 Chinese clonal tea cultivars using SSR markers provides new insights into their pedigree relationships. Tree Genetics & Genomes, 11, 1-12. https://doi.org/10.1007/s11295-015-0914-6
  • Turgutoğlu, E. (2019). A Research to Obtain Tolerant New Mutant Genotypes via Mutation Breeding from Fortune Mandarin Against Alternaria Brown Spot Disease and Determination of Some Phytohormone Levels in These Mutant Genotypes. PhD Thesis, University of Akdeniz, Türkiye.
  • Turgutoğlu, E., Baktır, İ. (2019). In vitro and in vivo assessment of the sensitivity of some tangerine mutants to Alternaria alternata pv. Citri. Mediterranean Agricultural Sciences, 32(2), 117-120. https://doi.org/10.29136/mediterranean.452671
  • Turkish Statistical Institute (TSI). (2022). Tea Production Data Portal (tuik.gov.tr), 25.05.2024.
  • Udage, A.C. (2021). Introduction to plant mutation breeding: different approaches and mutagenic agents. The Journal of Agricultural Sciences Sri Lanka, 16(3), 466-483. http://doi.org/10.4038/jas.v16i03.9472
  • URL-1, (2024). https://nucleus.iaea.org/sites/mvd/SitePages/Variety.aspx, October 5, 2024.
  • Wi S.G., Chung B.Y., Kim J.S. (2007). Effects of gamma irradiation on morphological changes and biological responses in plants. Micron, 38, 553-564. https://doi.org/10.1016/j.micron.2006.11.002
  • Yang, Y.J., Yang, S.J., Yang, Y.H., Zeng, J.M. (2003). The breeding of an early sprouting and high quality new clone suitable for fine gren tea. Journal China Tea, 25(2), 13–15.
  • Yarar, G., Kocak, M., Denli, N., Cavagnaro, P.F., Yildiz, M. (2022). Determination of the effective radiation dose for mutation breeding in purple carrot (Daucus carota L.) and possible variations formed. Molecular Biology Reports, 49(6), 5219-5228. https://doi.org/10.1007/s11033-021-06618-0
  • Yazici, L., Çiçek, S., Küçüktaban, F., Çoban, M., Tuncel, N. (2016). Determination of Appropirate Gamma Ray Dose and Effect on Seedlig Growth in M1 of Different Gamma Ray Dose in Cotton (Gossypium hirsitum L.) Variety Nazilli 663. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, 25(ÖZEL SAYI-2), 88-93. https://doi.org/10.21566/tarbitderg.281862
  • Yılmaz, A., Soysal, Ö. (2021). Use of Mutation Breeding Techniques In Field Crops, 6th International Conference on Agriculture, Animal Science And Rural Development, May 16-18, 20
Toplam 53 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bahçe Bitkileri Yetiştirme ve Islahı (Diğer)
Bölüm Araştırma Makaleleri
Yazarlar

Burcu Göksu Karaoğlu 0000-0002-1019-6188

Yıldız Aka Kaçar 0000-0001-5314-7952

Kadriye Yaprak Kantoglu 0000-0002-7247-9116

Burak Kunter 0009-0001-8546-7480

Keziban Yazıcı 0000-0002-5957-053X

Yayımlanma Tarihi 29 Temmuz 2025
Gönderilme Tarihi 5 Eylül 2024
Kabul Tarihi 4 Mart 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 6 Sayı: 2

Kaynak Göster

APA Göksu Karaoğlu, B., Aka Kaçar, Y., Kantoglu, K. Y., Kunter, B., vd. (2025). Determination of Effective Mutation Dose (ED50) To Be Used In Variety Treatment In Tea Plant. Recep Tayyip Erdogan University Journal of Science and Engineering, 6(2), 569-579. https://doi.org/10.53501/rteufemud.1543862
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