Familial Mediterranean Fever disease (FMF) is an autosomal recessive genetic disease, as well as a disease that seriously impairs the quality of life, accompanied by the appearance of symptoms such as recurrent high fever, peritonitis and acute synovitis. In FMF disease, a mutation status in the MEFV gene is encountered. The presence of faulty protein synthesis as a result of this mutation mediates the emergence of the disease. It is thought that there may be a condition associated with apoptosis in the pathogenesis of this disease. Apoptosis is a form of cell death in which energy is expended and programmed with great importance in maintaining cellular balance. In this study, the levels of Caspase 3, Caspase 8, Caspase 9, Granzyme B and Apaf 1 from apoptosis-related proteins were analyzed using the ELISA method in serum samples obtained from FMF patients treated with colchicine and a healthy control group. There was no statistically significant difference between Caspase 3 (P=0, 111), Granzyme B (P=0, 304), Apaf 1 (P=0, 097) and Caspase 8 (P=0, 245) levels in the FMF patient group compared to the control group (p >0, 05). A statistically significant difference was found in the Caspase 9 levels in the FMF patient group (P=0.001) compared to the control group (p<0.05). In this study, in which we aim to reveal the relationship between FMF and apoptosis, we can predict that drug use mediates the stabilization of apoptosis rate after an attack. However, in order for the relationship between FMF and apoptosis to be revealed more clearly, other studies using other advanced methods are also needed. However, Caspase 3 protein (PDB IDs: 2XYG), Caspase 8 protein (PDB ID: 3KIQ), Caspase 9 protein (PDB ID: 2AR9), Granzyme B protein (PDB ID: 1FQ3), and Apaf 1 protein (PDB ID: 3SDZ) were all subjected to an investigation to determine the effects of the chemical Colchicine. Research using the ADME/T method was carried out on the compounds that demonstrated the highest levels of activity.
Familial Mediterranean Fever Colchicine Apoptosis Caspases Granzyme B Apaf-1 İn silico
Birincil Dil | İngilizce |
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Konular | Moleküler Görüntüleme |
Bölüm | Research Article |
Yazarlar | |
Erken Görünüm Tarihi | 23 Haziran 2025 |
Yayımlanma Tarihi | |
Gönderilme Tarihi | 22 Mayıs 2025 |
Kabul Tarihi | 3 Haziran 2025 |
Yayımlandığı Sayı | Yıl 2025 Cilt: 9 Sayı: 5 |
Journal Full Title: Turkish Computational and Theoretical Chemistry
Journal Abbreviated Title: Turkish Comp Theo Chem (TC&TC)