Araştırma Makalesi
Fraser Photinia shoot explantation in vitro: effects of two distinct gamma-ray sources and identification of the optimal mutation dose
Öz
Because of its beautiful qualities and ability to withstand harsh conditions, Fraser photinia (Photinia × fraseri cv. Red Robin) is frequently used as an ornamental plant in garden designs. The efforts to create new, highly marketable variations of the species have begun to increase in response to the growing ability of the current kinds to adapt to changing climatic circumstances. For this species, which is susceptible to in vitro propagation, the in vitro mutation breeding technique holds significant promise for increasing the current variety. It is essential to ascertain whether ionizing gamma ray sources are suitable for in vitro mutation investigations on Fraser photinia. In order to do this, in vitro shoot explants were exposed to a total of thirteen different doses of radiation using 60Co (dosage rate: 235 Gy/h) and 137Cs (dosage rate: 821 Gy/h) gamma ray sources. The number of leaves and shoot length in in vitro plantlets were assessed thirty days after irradiation, and linear regression analysis was used to get the EMD50 values. Based on the quantity of leaves, the EMD50 dose for the cesium-137 source ray application was 60.34 Gy, whereas the cobalt-60 source resulted in an EMD50 dose of 80.88 Gy. These findings demonstrated that the EMD50 dose difference was significantly impacted by the source power, irradiation duration, and the influence produced by the linear energy transfer value of the irradiation during tissue penetration.
Anahtar Kelimeler
Etik Beyan
No environmentally harmful application was made in this research.
Destekleyen Kurum
This research was supported by the Margeht Biotechnology Llc. Co.
Teşekkür
This research was supported by the Margeht Biotechnology Llc. Co. The authors express their sincere gratitude to the TENMAK NÜKEN for irradiation treatment.
Kaynakça
- Guney, K., Cetin, M., Sevik, H., Guney, K. B. (2016). Influence of germination percentage and morphological properties of some hormones practice on Lilium martagon L. seeds. Oxidation Communications 39(1): 466-474.
- Ayan, S., Sarsekova, D., Kenesaryuly, G., Yilmaz, E., Gülseven, O., Şahin, I. (2021). Accumulation of heavy metal pollution caused by traffic in forest trees in the park of Kerey and Janibek Khans of the city of NurSultan, Kazakhstan. Journal of Forest Science 67(7): 357-366. https://doi.org/10.17221/37/2021-JFS.
- Laaribya, S., Alaoui, A., Ayan, S., Dindaroglu, T. (2023). Changes in the potential distribution of Atlas cedar in Morocco in the twenty-first century according to the emission scenarios of RCP 4,5 and RCP 8,5. Forestist 74(3): 1-10.
- Bonaminio, V. P., Blazich, F. A. (1983). Response of Fraser’s photinia stem cuttings to selected rooting compounds. Journal of Environmental Horticulture 1(1): 9-11. https://doi.org/10.24266/0738-2898-1.1.9.
- Brickell, C. (1996). Enciclopedia de Plantas y Flores. Royal Horticultural Society, Grijalbo Mondadori, Verona, Italy, p.566.
- Anonymous (2024a). Photinia Red Robin Hedge Plants. https://www.hedgingplantsdirect.co.uk/ (access date: October 2024).
- Anonymous (2024b). Introducing...Photinia. Common name: Christmas berry, red robin. https://www.rhs.org. uk/plants/photinia. (access date: October 2024).
- Anonymous (2024f). Photinia × fraseri “Red Robin.” (n.d.). Missouri Botanical Garden. (https:// www.missouribotanicalgarden.org/PlantFinder/ PlantFinderDetails.aspx?taxonid=271346). (access date: October 2024)Anonymous (2024d). Photinia fraseri red robin wall (ROSACEAE) Çit formlu alev çalısı. https://katalog. smsmarmaragroup.com/photinia-fraseri-red-robin-wallrosaceae/cit-formlu-alev-calisi. (access date: October 2024).
- [Anonymous (2024c). Thriving in the Elements: Choosing Photinia ‘Red Robin’ for Extreme Climates. https://duskhedges.co.nz/test-post-2/ (access date: October 2024).
- Anonymous (2024e). Photinia ‘Red Robin’ - How To Grow This Extremely Popular, Glossy-Leaved Cultivar? Horticulture Magazine, https://horticulture.co.uk/ photinia-red-robin/ (access date: October 2024).
- Tosca, A., Valagussa, M., Martinetti, L., Frangi, P. (2021). Biochar and green compost as peat alternatives in the cultivation of photinia and olive tree. Acta Horticulturae 1305: 257-262 https://doi.org/10.17660/ ActaHortic.2021.1305.35.
- [Anonymous (2024g). Best Soil for Your Red Tip Photinia. https://greg.app/red-tip-photinia-soil/ (access date: October 2024).
- Özel, H. B., Yücedağ, C., Ayan, S. (2023). Growth performances of Photinia × fraseri Dress. seedlings from cuttings of five ortets in different districts. SilvaWorld, 2(2), 60-65. https://doi.org/10.61326/silvaworld.v2i2.23.
- Anonymous (1977). Manual on Mutation Breeding. International Atomic Energy Agency, Technical Report Series No:119. Vienna, 290p.
- Waycott, W., Fort, S. B. T., Ryder, E. J. (1995). Inheritance of dwarfing genes in Lactuca sativa L. Journal of Heredity 86(1): 39-44.
- Sağel, Z., Peşkircioğlu, H., Tutluer, I., Uslu, N., Senay, A., Taner, K. Y., Kunter, B., Sekerci, S., Yalcin, S. (2002). Bitki Islahında Mutasyon ve Doku Kültürü Teknikleri, III: Ulusal Mutasyon Kursu Kurs Notları, TAEK, ANTHAM Nükleer Tarım Radyobiyoloji Bölümü, Ankara (in Turkish).
- Çakın, I., Kunter, B., Kantoglu, K. Y., Göktuğ, A., Akyüz Çağdaş, E., Ellialtıoğlu, Ş. Ş. (2025). In vitro mutasyon islahı çalışmalarına yönelik olarak farklı gama işını kaynaklarının kalanşo (Kalanchoe blossfeldiana Poelnn.) Yaprak ayası eksplantı üzerine etkilerinin karşılaştırılması. Bahçe, 54(Özel Sayı 1), 312-318. https://doi.org/10.53471/bahce.1539465
- Schum, A. (2003). Mutation Breeding in Ornamentals: An Efficient Breeding Method. Proj., 21st IS on Classical Molecular Breeding (Ed. G. Forkmann et al.), Acta. Hort. 612: 47-53.
- Maliga, P. (1984). Isolation and characterization of mutants in plant cell cultures. Annual Review of Plant Physiology 35: 519-552.
- Ahloowalia, B. S. (1995). In vitro mutagenesis for the improvement of vegetatively propagated plants. In: Induced Mutations and Molecular Techniques for Crop Improvement, Proceedings of IAEA/FAO Symposium. Vienna 19-23 June 1995, pp 531-541.
- Ahloowalia B. S., Maluszynski M. (2001). Induced Mutations- A new paradigm in plant breeding. EuphyticaKane, M. E., Sheehan, T. J., Philman, N. L. (1988). A micropropagation protocol using Fraser photinia for mutation induction and new cultivar selection. Proceedings of the Florida State Horticultural Society 100:334-337. (https://www.cabidigitallibrary.org/doi/ full/10.5555/19890354891).
- MVD (2024). Mutant Variety database. https://nucleus. iaea.org/sites/mvd/SitePages/Search.aspx (access date: May 2024).
- Farkash, E. A., Kao, G. D., Horman, S. R., Prak, E. T. L. (2006). Gamma irradiation increases endonucleasedependent L1 retrotranspositions in a cultured cell assay. Nucleic Acids Research 33(4): 1196-1204.
- Lagoda, P., Shu, Q., Foster, B., Nakagawa, H., Nakagawa, H. (2012). Effects of radiation on living cells and plant. Plant Mutation Breeding and Biotechnology. CABI Publishing, Wallinford, 123-124.
- Spencer-Lopes, M. M., Foster, B. P., Jankuloski, L. (2018). Manual on mutation breeding third edition. FAO Publishing, Italy, 301 pp.
- Turan Büyükdinç, D., Kantoğlu, K. Y., Karataş, A., İpek, A., Ellialtıoğlu, Ş. Ş. (2019). determination of effective mutagen dose for carrot (Daucus carota ssp. sativus var. atrorubens Alef and D. carota) callus cultures. International Journal of Scientific and Technological Research 5 (3): 15-23. https://doi.org/10.7176/ JSTR/5-3-02.
- Kantoglu, K. Y., Sarıtoprak, O., Akyüz Çağdaş, E., Okutan, E., Aktaş, H., Ellialtıoğlu, Ş. Ş. (2024). Kalanşoda (Kalanchoe blossfeldiana Poelnn.) in vitro mutasyon ıslahı. Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi, 20(Özel Sayı), 31-43. https://doi. org/10.58816/duzceod.1537178
- Larraburu, E. E., Carletti, S. M., Rodríguez Cáceres, E. A., Llorente, B. E. (2007). Micropropagation of photinia employing rhizobacteria to promote root development. Plant Cell Rep. Jun;26(6):711-7. doi: 10.1007/s00299- 006-0279-2. Epub 2007 Jan 5. PMID: 17205338.
- Gamborg, O. L., Miller, R. A., Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151- 158. https://doi. org/10.1016/0014-4827(68)90403-5.
- Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures, Physiol. Plant, 15: 473- 497.
- Haspolat, G. (2022). Induction of mutagenesis on Chrysanthemums. Ornamental Horticult. 28(4): 431- 441. https://doi.org/10.1590/2447-536X.v28i4.2523.
- Melsen, K., van de Wouw, M., Contreras, R. (2021). Mutation breeding in ornamentals. HortScience 56(10): 1154-1165. 2021.
- Micke, A., Donini, B. (1993). Induced mutations. Eds. M.D., Hayward, N., O., Bosemark, I., Romagosa. Plant Breeding, Principles and Prospects, Chapman & Hall, London. p52-62.
- Mba, C., Afza, R., Shu, Q., Shu, Q., Foster, B., Nakagowa, H. (2012). Mutagenic radiation: X-ray, ionizing particles and ultraviolet. Plant Mutation Breeding and Biotechnology. CABI, Oxfordshire, UK. 83-90.
- Leonard, A., Jacquet, P., Lauwerys, R. (1983). Mutagenicity and teratogenicity of mercury compounds. Mutation Research/Reviews in Genetic Toxicology 11(4): 1-18.
Farklı Gama Kaynaklarının In Vitro Fraser photinia Sürgün Explantları Üzerine Etkisi ve Etkili Mutasyon Dozunun Belirlenmesi
Öz
Fraser photinia (Photinia × fraseri cv. Red Robin), çevresel dayanıklılığı ve dekoratif özelliği nedeniyle bahçe tasarımlarında süs bitkisi olarak yaygın olarak tercih edilmektedir. Türün mevcut çeşitlerinin değişen iklim koşullarına uyum kapasitesinin artması göz önüne alındığında, pazara çekiciliği yüksek yeni çeşitlerin geliştirilmesi çabaları artmaya başlamıştır. In vitro çoğaltılmaya yatkın olan bu tür için in vitro mutasyon ıslah yöntemi, mevcut değişkenliği genişletmek için ciddi bir potansiyele sahiptir. Fraser photinia üzerinde in vitro mutasyon çalışmaları için iyonlaştırıcı gama ışını kaynaklarının uygulanabilirliğini belirlemek çok önemlidir. Bu çalışmada, in vitro sürgün eksplantlarının radyasyon duyarlılığını belirlemek için iki farklı gama iyonlaştırıcı radyasyon kaynağı sezyum 137 (137Cs) ve kobalt 60 (60Co) kullanılmış ve kaynaklar için etkili mutasyon dozu (EMD50) değerleri belirlenmiştir. Bu amaçla, in vitro sürgün eksplantları on üç farklı dozda (0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 130 Gy) 60Co (Doz hızı: 235 Gy/saat) ve 137Cs (Doz hızı: 821 Gy/saat) gama ışını kaynaklarıyla ışınlanmıştır. Işınlamadan otuz gün sonra, in vitro bitkiciklerde sürgün uzunluğu ve yaprak sayısı ölçülmüş ve EMD50 değerleri doğrusal regresyon analizi ile hesaplanmıştır. 137Cs kaynak ışını uygulaması sonucu yaprak sayısına dayalı EMD50 dozu 60.34 Gy olarak belirlenirken, 60Co kaynağı için bu değer 80.88 Gy olarak belirlenmiştir. Bu sonuçlara göre kaynak gücünün, ışınlama süresinin ve ışının dokuya nüfuz etmesi sırasında ışının doğrusal enerji transfer değerinin oluşturduğu etkinin EMD50 doz farkı üzerinde etkili olduğu görülmüştür.
Anahtar Kelimeler
Kaynakça
- Guney, K., Cetin, M., Sevik, H., Guney, K. B. (2016). Influence of germination percentage and morphological properties of some hormones practice on Lilium martagon L. seeds. Oxidation Communications 39(1): 466-474.
- Ayan, S., Sarsekova, D., Kenesaryuly, G., Yilmaz, E., Gülseven, O., Şahin, I. (2021). Accumulation of heavy metal pollution caused by traffic in forest trees in the park of Kerey and Janibek Khans of the city of NurSultan, Kazakhstan. Journal of Forest Science 67(7): 357-366. https://doi.org/10.17221/37/2021-JFS.
- Laaribya, S., Alaoui, A., Ayan, S., Dindaroglu, T. (2023). Changes in the potential distribution of Atlas cedar in Morocco in the twenty-first century according to the emission scenarios of RCP 4,5 and RCP 8,5. Forestist 74(3): 1-10.
- Bonaminio, V. P., Blazich, F. A. (1983). Response of Fraser’s photinia stem cuttings to selected rooting compounds. Journal of Environmental Horticulture 1(1): 9-11. https://doi.org/10.24266/0738-2898-1.1.9.
- Brickell, C. (1996). Enciclopedia de Plantas y Flores. Royal Horticultural Society, Grijalbo Mondadori, Verona, Italy, p.566.
- Anonymous (2024a). Photinia Red Robin Hedge Plants. https://www.hedgingplantsdirect.co.uk/ (access date: October 2024).
- Anonymous (2024b). Introducing...Photinia. Common name: Christmas berry, red robin. https://www.rhs.org. uk/plants/photinia. (access date: October 2024).
- Anonymous (2024f). Photinia × fraseri “Red Robin.” (n.d.). Missouri Botanical Garden. (https:// www.missouribotanicalgarden.org/PlantFinder/ PlantFinderDetails.aspx?taxonid=271346). (access date: October 2024)Anonymous (2024d). Photinia fraseri red robin wall (ROSACEAE) Çit formlu alev çalısı. https://katalog. smsmarmaragroup.com/photinia-fraseri-red-robin-wallrosaceae/cit-formlu-alev-calisi. (access date: October 2024).
- [Anonymous (2024c). Thriving in the Elements: Choosing Photinia ‘Red Robin’ for Extreme Climates. https://duskhedges.co.nz/test-post-2/ (access date: October 2024).
- Anonymous (2024e). Photinia ‘Red Robin’ - How To Grow This Extremely Popular, Glossy-Leaved Cultivar? Horticulture Magazine, https://horticulture.co.uk/ photinia-red-robin/ (access date: October 2024).
- Tosca, A., Valagussa, M., Martinetti, L., Frangi, P. (2021). Biochar and green compost as peat alternatives in the cultivation of photinia and olive tree. Acta Horticulturae 1305: 257-262 https://doi.org/10.17660/ ActaHortic.2021.1305.35.
- [Anonymous (2024g). Best Soil for Your Red Tip Photinia. https://greg.app/red-tip-photinia-soil/ (access date: October 2024).
- Özel, H. B., Yücedağ, C., Ayan, S. (2023). Growth performances of Photinia × fraseri Dress. seedlings from cuttings of five ortets in different districts. SilvaWorld, 2(2), 60-65. https://doi.org/10.61326/silvaworld.v2i2.23.
- Anonymous (1977). Manual on Mutation Breeding. International Atomic Energy Agency, Technical Report Series No:119. Vienna, 290p.
- Waycott, W., Fort, S. B. T., Ryder, E. J. (1995). Inheritance of dwarfing genes in Lactuca sativa L. Journal of Heredity 86(1): 39-44.
- Sağel, Z., Peşkircioğlu, H., Tutluer, I., Uslu, N., Senay, A., Taner, K. Y., Kunter, B., Sekerci, S., Yalcin, S. (2002). Bitki Islahında Mutasyon ve Doku Kültürü Teknikleri, III: Ulusal Mutasyon Kursu Kurs Notları, TAEK, ANTHAM Nükleer Tarım Radyobiyoloji Bölümü, Ankara (in Turkish).
- Çakın, I., Kunter, B., Kantoglu, K. Y., Göktuğ, A., Akyüz Çağdaş, E., Ellialtıoğlu, Ş. Ş. (2025). In vitro mutasyon islahı çalışmalarına yönelik olarak farklı gama işını kaynaklarının kalanşo (Kalanchoe blossfeldiana Poelnn.) Yaprak ayası eksplantı üzerine etkilerinin karşılaştırılması. Bahçe, 54(Özel Sayı 1), 312-318. https://doi.org/10.53471/bahce.1539465
- Schum, A. (2003). Mutation Breeding in Ornamentals: An Efficient Breeding Method. Proj., 21st IS on Classical Molecular Breeding (Ed. G. Forkmann et al.), Acta. Hort. 612: 47-53.
- Maliga, P. (1984). Isolation and characterization of mutants in plant cell cultures. Annual Review of Plant Physiology 35: 519-552.
- Ahloowalia, B. S. (1995). In vitro mutagenesis for the improvement of vegetatively propagated plants. In: Induced Mutations and Molecular Techniques for Crop Improvement, Proceedings of IAEA/FAO Symposium. Vienna 19-23 June 1995, pp 531-541.
- Ahloowalia B. S., Maluszynski M. (2001). Induced Mutations- A new paradigm in plant breeding. EuphyticaKane, M. E., Sheehan, T. J., Philman, N. L. (1988). A micropropagation protocol using Fraser photinia for mutation induction and new cultivar selection. Proceedings of the Florida State Horticultural Society 100:334-337. (https://www.cabidigitallibrary.org/doi/ full/10.5555/19890354891).
- MVD (2024). Mutant Variety database. https://nucleus. iaea.org/sites/mvd/SitePages/Search.aspx (access date: May 2024).
- Farkash, E. A., Kao, G. D., Horman, S. R., Prak, E. T. L. (2006). Gamma irradiation increases endonucleasedependent L1 retrotranspositions in a cultured cell assay. Nucleic Acids Research 33(4): 1196-1204.
- Lagoda, P., Shu, Q., Foster, B., Nakagawa, H., Nakagawa, H. (2012). Effects of radiation on living cells and plant. Plant Mutation Breeding and Biotechnology. CABI Publishing, Wallinford, 123-124.
- Spencer-Lopes, M. M., Foster, B. P., Jankuloski, L. (2018). Manual on mutation breeding third edition. FAO Publishing, Italy, 301 pp.
- Turan Büyükdinç, D., Kantoğlu, K. Y., Karataş, A., İpek, A., Ellialtıoğlu, Ş. Ş. (2019). determination of effective mutagen dose for carrot (Daucus carota ssp. sativus var. atrorubens Alef and D. carota) callus cultures. International Journal of Scientific and Technological Research 5 (3): 15-23. https://doi.org/10.7176/ JSTR/5-3-02.
- Kantoglu, K. Y., Sarıtoprak, O., Akyüz Çağdaş, E., Okutan, E., Aktaş, H., Ellialtıoğlu, Ş. Ş. (2024). Kalanşoda (Kalanchoe blossfeldiana Poelnn.) in vitro mutasyon ıslahı. Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi, 20(Özel Sayı), 31-43. https://doi. org/10.58816/duzceod.1537178
- Larraburu, E. E., Carletti, S. M., Rodríguez Cáceres, E. A., Llorente, B. E. (2007). Micropropagation of photinia employing rhizobacteria to promote root development. Plant Cell Rep. Jun;26(6):711-7. doi: 10.1007/s00299- 006-0279-2. Epub 2007 Jan 5. PMID: 17205338.
- Gamborg, O. L., Miller, R. A., Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151- 158. https://doi. org/10.1016/0014-4827(68)90403-5.
- Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures, Physiol. Plant, 15: 473- 497.
- Haspolat, G. (2022). Induction of mutagenesis on Chrysanthemums. Ornamental Horticult. 28(4): 431- 441. https://doi.org/10.1590/2447-536X.v28i4.2523.
- Melsen, K., van de Wouw, M., Contreras, R. (2021). Mutation breeding in ornamentals. HortScience 56(10): 1154-1165. 2021.
- Micke, A., Donini, B. (1993). Induced mutations. Eds. M.D., Hayward, N., O., Bosemark, I., Romagosa. Plant Breeding, Principles and Prospects, Chapman & Hall, London. p52-62.
- Mba, C., Afza, R., Shu, Q., Shu, Q., Foster, B., Nakagowa, H. (2012). Mutagenic radiation: X-ray, ionizing particles and ultraviolet. Plant Mutation Breeding and Biotechnology. CABI, Oxfordshire, UK. 83-90.
- Leonard, A., Jacquet, P., Lauwerys, R. (1983). Mutagenicity and teratogenicity of mercury compounds. Mutation Research/Reviews in Genetic Toxicology 11(4): 1-18.
Toplam 35 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Radyasyon Teknolojisi |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 29 Haziran 2025 |
| Gönderilme Tarihi | 5 Haziran 2025 |
| Kabul Tarihi | 26 Haziran 2025 |
| Yayımlandığı Sayı | Yıl 2025 Cilt: 38 Sayı: 1 |
