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Yıl 2025, Cilt: 12 Sayı: 3, 565 - 573, 23.07.2025

Khalid Rasooly , Songül Yalçın Ateş , Burak Özgören , Ali Ferhan Morca , Ali Çelik

https://doi.org/10.30910/turkjans.1665625

Öz

Kaynakça

  • Akbaş B., Değirmenci K., Çiftçi O., Kaya A., Yurtmen M., Uzunoğullari N., Türkölmez Ş., 2011. Update on Plum pox virus distribution in Turkey. Phytopathologia Mediterranea, 50 (1), 75-83.
  • Akbaş B., Morca A. F., Coşkan S., Santosa A. I., Çulal-Kılıç H., Çelik A., 2023. First complete sequences and genetic variation of plum pox virus T strain in Prunus dulcis and Prunus cerasus. 3 Biotech, 13 (10), 332.
  • Atanasoff, D. (1932). Plum pox. A new virus disease. Cook, J., Aydin-Schmidt, B., González, I. J., Bell, D., Edlund, E., Nassor, M. H., ... & Björkman, A. (2015). Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar. Malaria journal, 14, 1-6.
  • Cook J., Aydin-Schmidt B., González I. J., Bell D., Edlund E., Nassor M. H., Msellem M., Ali A., Abass K., Mårtensson A., Björkman, A., 2015. Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar. Malaria journal, 14, 1-6.
  • Candresse T., Svanella-Dumas L., Gentit P., Caglayan K., Çevik, B., 2007. First report of the presence of Plum pox virus Rec strain in Turkey. Plant Disease, 91 (3), 331-331.
  • Cambra M., Boscia D., Myrta A., Palkovics L., Navrátil M., Barba M., Capote N., 2006. Detection and characterization of Plum pox virus: serological methods. EPPO Bulletin, 36(2), 254-26.
  • Cambra M., Madarıaga M., Varverı C., Çağlayan K., Morca A. F., Chırkov S., Glasa M., 2024. Estimated costs of plum pox virus and management of sharka, the disease it causes. Phytopathologia Mediterranea, 343-365.
  • Coşkan S., Morca A. F., Akbaş B., Çelik A., Santosa A. I., 2022. Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey. Journal of Plant Diseases and Protection, 129(4), 981-991.
  • Candresse, T., Macquaire, G., Lanne, M., Bousalem, M., Quiot-Douine, L., Quiot, J. B., & Dunez, J. (1994, June). Analysis of plum pox virus variability and development of strain-specific PCR assays. In XVI International Symposium on Fruit Tree Virus diseases 386 (pp. 357-369).
  • Çelik A., 2020. Bursa, Bilecik ve Bolu illerinde şeftali üretim alanlarında görülen bazı viral hastalıkların serolojik ve moleküler karakterizasyonu. Ankara Fen Bilimleri Enstitüsü Doktora Tezi.
  • Çelik A., and Morca A. F., 2021. Development of colorimetric and real time loop-mediated isothermal amplification (cr-LAMP) assay for rapid detection of Wheat dwarf virus (WDV). Crop Protection, 149, 105786.
  • Çelik A., Çakar D., Derviş S., Morca A. F., Akıllı-Şimşek S., Romon-Ochoa P., Özer G., 2024. New Detection Methods for Cryphonectria Hypovirus 1 (CHV1) through SYBR Green-Based Real-Time PCR and Loop-Mediated Isothermal Amplification (LAMP). Viruses, 16(8), 1203.
  • Çelik, A., & Ertunç, F. (2021). Reverse transcription loop-mediated isothermal amplification (RT-LAMP) of plum pox potyvirus Turkey (PPV-T) strain. Journal of Plant Diseases and Protection, 128(3), 663-671.
  • Elibüyük İ. Ö., 2003. Natural spread of Plum pox virus in Ankara, Turkeye. Journal of Phytopathology, 151(11‐12), 617-619.
  • Fernandez-Soto, P., Gandasegui Arahuetes, J., Sanchez Hernandez, A., Lopez Aban, J., Vicente Santiago, B., & Muro, A. (2014). A loop-mediated isothermal amplification (LAMP) assay for early detection of Schistosoma mansoni in stool samples: a diagnostic approach in a murine model. PLoS neglected tropical diseases, 8(9), e3126.
  • Galitelli, D., & Minafra, A. (1994). Electropheresis. Course on Plant Viruses Diagnosis. University of Cukurova, Dept. Plant Protection. Adana, Turkey, 89-114.
  • Golabi M., Flodrops M., Grasland B., Vinayaka A. C., Quyen T. L., Nguyen, T., Bank D.D., Wolff A., 2021. Development of reverse transcription loop-mediated isothermal amplification assay for rapid and on-site detection of avian influenza virus. Frontiers in Cellular and Infection Microbiology, 11, 652048.
  • Garcia, J. A., Glasa, M., Cambra, M., & Candresse, T. (2014). Plum pox virus and sharka: a model potyvirus and a major disease. Molecular plant pathology, 15(3), 226-241.
  • Gürcan K., and Ceylan A., 2016. Strain identification and sequence variability of plum pox virus in Turkey. Turkish Journal of Agriculture and Forestry, 40(5), 746-760.
  • Gürcan K., Teber S., Çağlayan K., 2019. Further investigation of a genetically divergent group of plum pox virus-M strain in Turkey. Journal of Plant Pathology, 101, 385-391.
  • Gürcan K., Teber S., Candresse T., 2020. Genetic analysis suggests a long and largely isolated evolutionary history of plum pox virus strain D in Turkey. Plant Pathology, 69(2), 370-378.
  • Hadersdorfer J., Neumüller M., Treutter D., Fischer T. C., 2011. Fast and reliable detection of Plum pox virus in woody host plants using the Blue LAMP protocol. Annals of Applied Biology, 159(3), 456-466.
  • Iftikhar R., Moyo L., Pappu H. R., 2020. A loop‐mediated isothermal amplification assay for the detection of Dahlia mosaic caulimovirus in Dahlia (Dahlia variabilis). Annals of Applied Biology, 176(2), 203-209.
  • Jawla J., Kumar R. R., Mendiratta S. K., Agarwal R. K., Singh P., Saksona, V., Kumari S., Kumar, D., 2023. A novel paper based loop mediated isothermal amplification and lateral flow assay (LAMP‐LFA) for point‐of‐care detection of buffalo tissue origin in diverse foods. Journal of Food Safety, 43(3), e13038.
  • Kaarj K., Akarapipad P., Yoon J. Y., 2018. Simpler, faster, and sensitive Zika virus assay using smartphone detection of loop-mediated isothermal amplification on paper microfluidic chips. Scientific reports, 8(1), 12438. Kurçman S., 1973. Detection of sharka virus on apricot and plum trees in Ankara. Journal of Turkish Phytopathology, 2, 124-129.
  • Koç G., 2010. Doğu Akdeniz Bölgesinde Sert Çekirdekli Meyvelerde Plum pox potyvirus (PPV, Sharka)’ünün Durumunun Belirlenmesi ve Karakterizasyonu. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Doktora Tezi.
  • Lalli M. A., Langmade J. S., Chen X., Fronick C. C., Sawyer C. S., Burcea L. C., Wilkinson M.N., Fulton R.S., Heinz M., Buchser W. J., Hea R. D., Mitra R. D.,Milbrandt J., 2021. Rapid and extraction-free detection of SARS-CoV-2 from saliva by colorimetric reverse-transcription loop-mediated isothermal amplification. Clinical chemistry, 67(2), 415-424.
  • Laín, S., Riechmann, J., Martín, M. T., & Garcia, J. A. (1989). Homologous potyvirus and flavivirus proteins belonging to a superfamily of helicase-like proteins. Gene, 82(2), 357-362.
  • Levy L., Damsteegt V., Welliver R., 2000. First report of Plum pox virus (Sharka disease) in Prunus persica in the United States. Plant Disease, 84(2), 202-202.
  • Lopez-Moya J. J., Fernández-Fernández M. R., Cambra M., Garcı́a, J. A., 2000. Biotechnological aspects of plum pox virus. Journal of Biotechnology, 76(2-3), 121-136.
  • Moehling T. J., Choi G., Dugan L. C., Salit M., Meagher R. J., 2021. LAMP diagnostics at the point-of-care: emerging trends and perspectives for the developer community. Expert Review of Molecular Diagnostics, 21(1), 43-61.
  • Morca,A. F., Coşkan S., Öncü F., 2020. Determination and partial molecular characterization of Plum pox virus in Bolu province. Plant Protection Bulletin, 60(4), 59-68.
  • Morca A. F., Coşkan S., Çelik A., 2021. Burdur ilinde Plum pox virus’ un tespiti ve kısmi kılıf protein geninin moleküler karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(4), 805-814.
  • Morca A. F., Coşka, S., Akbaş, B., 2022. Zonguldak İlinde Plum pox virus’ un Tespiti, Karakterizasyonu ve Takibi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(6), 1369-1377.
  • Martin, L. M., Leff, M., Calonge, N., Garrett, C., & Nelson, D. E. (2000). Validation of self-reported chronic conditions and health services in a managed care population. American journal of preventive medicine, 18(3), 215-218.
  • Navratil M., Safarova D., Karesova R., Petrzik K., 2005. First incidence of Plum pox virus on apricot trees in China. Plant Disease, 89(3), 338-338.
  • Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., & Hase, T. (2000). Loop-mediated isothermal amplification of DNA. Nucleic acids research, 28(12), e63-e63.
  • Peltzer D., Tobler K., Fraefel C., Maley M., Bachofen C., 2021. Rapid and simple colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Bovine alphaherpesvirus 1. Journal of Virological Methods, 289, 114041.
  • Panno, S., Matić, S., Tiberini, A., Caruso, A. G., Bella, P., Torta, L., ... & Davino, S. (2020). Loop mediated isothermal amplification: principles and applications in plant virology. Plants, 9(4), 461.
  • Sahtiyanci S., 1969. Virus de la sharka chez le prunier. Bulletin phytosanitaire FAO, 17, 69.
  • Sarkes A., Fu H., Feindel D., Harding M., Feng J., 2020. Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of Tomato brown rugose fruit virus (ToBRFV). PLoS One, 15(6), e0230403.
  • Salvador, B., García, J. A., & Simón‐Mateo, C. (2006). Causal agent of sharka disease: Plum pox virus genome and function of gene products. EPPO Bulletin, 36(2), 229-238.
  • Serçe Ç. U., Candresse T., Svanella-Dumas L., Krizbai L., Gazel M., Çağlayan K., 2009. Further characterization of a new recombinant group of Plum pox virus isolates, PPV-T, found in orchards in the Ankara province of Turkey. Virus Research, 142(1-2), 121-126.
  • Serçe Ç. U., Gazel M., Çağlayan K., 2011. Plum pox virus streynlerinin Türkiye‟ deki Dağılımı (Distribution of Plum pox virus strains in Turkey), Türkiye IV. Bitki Koruma Kongresi Bildirileri, Kahramanmaraş, 72.
  • Spiegel S., Kovalenko E. M., Varga A., James D., 2004. Detection and partial molecular characterization of two Plum pox virus isolates from plum and wild apricot in southeast Kazakhstan. Plant disease, 88(9), 973-979.
  • Stehlíková D., Beran P., Cohen S. P., Čurn V., 2020. Development of real-time and colorimetric loop mediated isothermal amplification assay for detection of Xanthomonas gardneri. Microorganisms, 8(9), 1301.
  • Suzuki R., Fukuta S., Matsumoto Y., Hasegawa T., Kojima H., Hotta M., Miyake N., 2016. Development of reverse transcription loop-mediated isothermal amplification assay as a simple detection method of Chrysanthemum stem necrosis virus in chrysanthemum and tomato. Journal of virological methods, 236, 29-34.
  • Teber S., Ceylan A., Gürcan K., Candresse T., Ulubaş-Serçe Ç., Akbulut M., Akbaş B., 2019. Genetic diversity and molecular epidemiology of the T strain of Plum pox virus. Plant Pathology, 68(4), 755-763.
  • Teber S., Gürcan K., Brevet M., Palmisano F., Candresse T., Dallot S., 2023. High genetic diversity suggests a long evolutionary history of plum pox virus‐M in Turkey. Plant Pathology, 72(2), 300-311.
  • Thompson D., McCann M., MacLeod M., Lye D., Green M., James D., 2001. First report of plum pox potyvirus in Ontario, Canada. Plant Disease, 85(1), 97-97.
  • Varveri C., Ravelonandro M., Dunez J., 1987. Construction and use of a cloned cDNA probe for the detection of plum pox virus in plants. Phytopathology, 77(8), 1221-1224.
  • Varga A., and James D., 2006. Use of reverse transcription loop-mediated isothermal amplification for the detection of Plum pox virus. Journal of Virological Methods, 138(1-2), 184-190.
  • Zhao L. M., Li G., Gao Y., Zhu Y. R., Liu J., Zhu, X. P., 2015. Reverse transcription loop-mediated isothermal amplification assay for detecting tomato chlorosis virus. Journal of virological methods, 213, 93-97.
  • Zotto A. D., Ortego J. M., Raigón J. M., Caloggero S., Rossini M., Ducasse D. A., 2006. First report in Argentina of Plum pox virus causing sharka disease in Prunus. Plant Disease, 90(4), 523-523.

Performance of Ready-To-Use Isothermal Detection Kits in Plum pox virus (PPV) Detection

Yıl 2025, Cilt: 12 Sayı: 3, 565 - 573, 23.07.2025

Khalid Rasooly , Songül Yalçın Ateş , Burak Özgören , Ali Ferhan Morca , Ali Çelik

https://doi.org/10.30910/turkjans.1665625

Öz

Plum pox virus (Potyvirus plumpoxi-PPV), one of the most significant diseases affecting stone fruit trees, has been extensively studied worldwide since its initial detection in 1917, with various diagnostic approaches explored. This study investigates the performance of colorimetric and real-time diagnostic kits developed by DOSA Information and Communication Technologies Ltd. for detecting PPV under isothermal conditions. These isothermal diagnostic kits, developed and optimized with domestic resources, stand out as highly practical diagnostic tools, leveraging an advantage of containing proprietary primer sets that enable direct testing of RNA from infected plants, independent of existing isothermal methods. In this study, the isothermal diagnostic kits enabled successful amplification of PPV at 65°C within 60 minutes, without cross-reaction with potential host genomes or other viruses that could co-infect. The colorimetric and real-time diagnostic kits demonstrated detection sensitivities of 10-4 and 10-5 ng/µL of PPV RNA, respectively, meeting the desired standards for PPV detection. These kits also facilitated effective amplification of PPV-M, D, and T strains, commonly found in Türkiye. Colorimetric and real-time isothermal diagnostic kits can be utilized as rapid and cost-effective tools in PPV screening programs. The application of these kits, especially in field settings and areas with limited laboratory infrastructure, should be encouraged. This successfully developed and optimized isothermal diagnostic platform could also be adapted for the detection of other significant plant viruses, offering a strategic advantage in managing viral diseases in agricultural regions such as Türkiye.

Anahtar Kelimeler

PPV Sharka Disease Isotermal LAMP Kit

Kaynakça

  • Akbaş B., Değirmenci K., Çiftçi O., Kaya A., Yurtmen M., Uzunoğullari N., Türkölmez Ş., 2011. Update on Plum pox virus distribution in Turkey. Phytopathologia Mediterranea, 50 (1), 75-83.
  • Akbaş B., Morca A. F., Coşkan S., Santosa A. I., Çulal-Kılıç H., Çelik A., 2023. First complete sequences and genetic variation of plum pox virus T strain in Prunus dulcis and Prunus cerasus. 3 Biotech, 13 (10), 332.
  • Atanasoff, D. (1932). Plum pox. A new virus disease. Cook, J., Aydin-Schmidt, B., González, I. J., Bell, D., Edlund, E., Nassor, M. H., ... & Björkman, A. (2015). Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar. Malaria journal, 14, 1-6.
  • Cook J., Aydin-Schmidt B., González I. J., Bell D., Edlund E., Nassor M. H., Msellem M., Ali A., Abass K., Mårtensson A., Björkman, A., 2015. Loop-mediated isothermal amplification (LAMP) for point-of-care detection of asymptomatic low-density malaria parasite carriers in Zanzibar. Malaria journal, 14, 1-6.
  • Candresse T., Svanella-Dumas L., Gentit P., Caglayan K., Çevik, B., 2007. First report of the presence of Plum pox virus Rec strain in Turkey. Plant Disease, 91 (3), 331-331.
  • Cambra M., Boscia D., Myrta A., Palkovics L., Navrátil M., Barba M., Capote N., 2006. Detection and characterization of Plum pox virus: serological methods. EPPO Bulletin, 36(2), 254-26.
  • Cambra M., Madarıaga M., Varverı C., Çağlayan K., Morca A. F., Chırkov S., Glasa M., 2024. Estimated costs of plum pox virus and management of sharka, the disease it causes. Phytopathologia Mediterranea, 343-365.
  • Coşkan S., Morca A. F., Akbaş B., Çelik A., Santosa A. I., 2022. Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey. Journal of Plant Diseases and Protection, 129(4), 981-991.
  • Candresse, T., Macquaire, G., Lanne, M., Bousalem, M., Quiot-Douine, L., Quiot, J. B., & Dunez, J. (1994, June). Analysis of plum pox virus variability and development of strain-specific PCR assays. In XVI International Symposium on Fruit Tree Virus diseases 386 (pp. 357-369).
  • Çelik A., 2020. Bursa, Bilecik ve Bolu illerinde şeftali üretim alanlarında görülen bazı viral hastalıkların serolojik ve moleküler karakterizasyonu. Ankara Fen Bilimleri Enstitüsü Doktora Tezi.
  • Çelik A., and Morca A. F., 2021. Development of colorimetric and real time loop-mediated isothermal amplification (cr-LAMP) assay for rapid detection of Wheat dwarf virus (WDV). Crop Protection, 149, 105786.
  • Çelik A., Çakar D., Derviş S., Morca A. F., Akıllı-Şimşek S., Romon-Ochoa P., Özer G., 2024. New Detection Methods for Cryphonectria Hypovirus 1 (CHV1) through SYBR Green-Based Real-Time PCR and Loop-Mediated Isothermal Amplification (LAMP). Viruses, 16(8), 1203.
  • Çelik, A., & Ertunç, F. (2021). Reverse transcription loop-mediated isothermal amplification (RT-LAMP) of plum pox potyvirus Turkey (PPV-T) strain. Journal of Plant Diseases and Protection, 128(3), 663-671.
  • Elibüyük İ. Ö., 2003. Natural spread of Plum pox virus in Ankara, Turkeye. Journal of Phytopathology, 151(11‐12), 617-619.
  • Fernandez-Soto, P., Gandasegui Arahuetes, J., Sanchez Hernandez, A., Lopez Aban, J., Vicente Santiago, B., & Muro, A. (2014). A loop-mediated isothermal amplification (LAMP) assay for early detection of Schistosoma mansoni in stool samples: a diagnostic approach in a murine model. PLoS neglected tropical diseases, 8(9), e3126.
  • Galitelli, D., & Minafra, A. (1994). Electropheresis. Course on Plant Viruses Diagnosis. University of Cukurova, Dept. Plant Protection. Adana, Turkey, 89-114.
  • Golabi M., Flodrops M., Grasland B., Vinayaka A. C., Quyen T. L., Nguyen, T., Bank D.D., Wolff A., 2021. Development of reverse transcription loop-mediated isothermal amplification assay for rapid and on-site detection of avian influenza virus. Frontiers in Cellular and Infection Microbiology, 11, 652048.
  • Garcia, J. A., Glasa, M., Cambra, M., & Candresse, T. (2014). Plum pox virus and sharka: a model potyvirus and a major disease. Molecular plant pathology, 15(3), 226-241.
  • Gürcan K., and Ceylan A., 2016. Strain identification and sequence variability of plum pox virus in Turkey. Turkish Journal of Agriculture and Forestry, 40(5), 746-760.
  • Gürcan K., Teber S., Çağlayan K., 2019. Further investigation of a genetically divergent group of plum pox virus-M strain in Turkey. Journal of Plant Pathology, 101, 385-391.
  • Gürcan K., Teber S., Candresse T., 2020. Genetic analysis suggests a long and largely isolated evolutionary history of plum pox virus strain D in Turkey. Plant Pathology, 69(2), 370-378.
  • Hadersdorfer J., Neumüller M., Treutter D., Fischer T. C., 2011. Fast and reliable detection of Plum pox virus in woody host plants using the Blue LAMP protocol. Annals of Applied Biology, 159(3), 456-466.
  • Iftikhar R., Moyo L., Pappu H. R., 2020. A loop‐mediated isothermal amplification assay for the detection of Dahlia mosaic caulimovirus in Dahlia (Dahlia variabilis). Annals of Applied Biology, 176(2), 203-209.
  • Jawla J., Kumar R. R., Mendiratta S. K., Agarwal R. K., Singh P., Saksona, V., Kumari S., Kumar, D., 2023. A novel paper based loop mediated isothermal amplification and lateral flow assay (LAMP‐LFA) for point‐of‐care detection of buffalo tissue origin in diverse foods. Journal of Food Safety, 43(3), e13038.
  • Kaarj K., Akarapipad P., Yoon J. Y., 2018. Simpler, faster, and sensitive Zika virus assay using smartphone detection of loop-mediated isothermal amplification on paper microfluidic chips. Scientific reports, 8(1), 12438. Kurçman S., 1973. Detection of sharka virus on apricot and plum trees in Ankara. Journal of Turkish Phytopathology, 2, 124-129.
  • Koç G., 2010. Doğu Akdeniz Bölgesinde Sert Çekirdekli Meyvelerde Plum pox potyvirus (PPV, Sharka)’ünün Durumunun Belirlenmesi ve Karakterizasyonu. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Doktora Tezi.
  • Lalli M. A., Langmade J. S., Chen X., Fronick C. C., Sawyer C. S., Burcea L. C., Wilkinson M.N., Fulton R.S., Heinz M., Buchser W. J., Hea R. D., Mitra R. D.,Milbrandt J., 2021. Rapid and extraction-free detection of SARS-CoV-2 from saliva by colorimetric reverse-transcription loop-mediated isothermal amplification. Clinical chemistry, 67(2), 415-424.
  • Laín, S., Riechmann, J., Martín, M. T., & Garcia, J. A. (1989). Homologous potyvirus and flavivirus proteins belonging to a superfamily of helicase-like proteins. Gene, 82(2), 357-362.
  • Levy L., Damsteegt V., Welliver R., 2000. First report of Plum pox virus (Sharka disease) in Prunus persica in the United States. Plant Disease, 84(2), 202-202.
  • Lopez-Moya J. J., Fernández-Fernández M. R., Cambra M., Garcı́a, J. A., 2000. Biotechnological aspects of plum pox virus. Journal of Biotechnology, 76(2-3), 121-136.
  • Moehling T. J., Choi G., Dugan L. C., Salit M., Meagher R. J., 2021. LAMP diagnostics at the point-of-care: emerging trends and perspectives for the developer community. Expert Review of Molecular Diagnostics, 21(1), 43-61.
  • Morca,A. F., Coşkan S., Öncü F., 2020. Determination and partial molecular characterization of Plum pox virus in Bolu province. Plant Protection Bulletin, 60(4), 59-68.
  • Morca A. F., Coşkan S., Çelik A., 2021. Burdur ilinde Plum pox virus’ un tespiti ve kısmi kılıf protein geninin moleküler karakterizasyonu. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 24(4), 805-814.
  • Morca A. F., Coşka, S., Akbaş, B., 2022. Zonguldak İlinde Plum pox virus’ un Tespiti, Karakterizasyonu ve Takibi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 25(6), 1369-1377.
  • Martin, L. M., Leff, M., Calonge, N., Garrett, C., & Nelson, D. E. (2000). Validation of self-reported chronic conditions and health services in a managed care population. American journal of preventive medicine, 18(3), 215-218.
  • Navratil M., Safarova D., Karesova R., Petrzik K., 2005. First incidence of Plum pox virus on apricot trees in China. Plant Disease, 89(3), 338-338.
  • Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., & Hase, T. (2000). Loop-mediated isothermal amplification of DNA. Nucleic acids research, 28(12), e63-e63.
  • Peltzer D., Tobler K., Fraefel C., Maley M., Bachofen C., 2021. Rapid and simple colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Bovine alphaherpesvirus 1. Journal of Virological Methods, 289, 114041.
  • Panno, S., Matić, S., Tiberini, A., Caruso, A. G., Bella, P., Torta, L., ... & Davino, S. (2020). Loop mediated isothermal amplification: principles and applications in plant virology. Plants, 9(4), 461.
  • Sahtiyanci S., 1969. Virus de la sharka chez le prunier. Bulletin phytosanitaire FAO, 17, 69.
  • Sarkes A., Fu H., Feindel D., Harding M., Feng J., 2020. Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of Tomato brown rugose fruit virus (ToBRFV). PLoS One, 15(6), e0230403.
  • Salvador, B., García, J. A., & Simón‐Mateo, C. (2006). Causal agent of sharka disease: Plum pox virus genome and function of gene products. EPPO Bulletin, 36(2), 229-238.
  • Serçe Ç. U., Candresse T., Svanella-Dumas L., Krizbai L., Gazel M., Çağlayan K., 2009. Further characterization of a new recombinant group of Plum pox virus isolates, PPV-T, found in orchards in the Ankara province of Turkey. Virus Research, 142(1-2), 121-126.
  • Serçe Ç. U., Gazel M., Çağlayan K., 2011. Plum pox virus streynlerinin Türkiye‟ deki Dağılımı (Distribution of Plum pox virus strains in Turkey), Türkiye IV. Bitki Koruma Kongresi Bildirileri, Kahramanmaraş, 72.
  • Spiegel S., Kovalenko E. M., Varga A., James D., 2004. Detection and partial molecular characterization of two Plum pox virus isolates from plum and wild apricot in southeast Kazakhstan. Plant disease, 88(9), 973-979.
  • Stehlíková D., Beran P., Cohen S. P., Čurn V., 2020. Development of real-time and colorimetric loop mediated isothermal amplification assay for detection of Xanthomonas gardneri. Microorganisms, 8(9), 1301.
  • Suzuki R., Fukuta S., Matsumoto Y., Hasegawa T., Kojima H., Hotta M., Miyake N., 2016. Development of reverse transcription loop-mediated isothermal amplification assay as a simple detection method of Chrysanthemum stem necrosis virus in chrysanthemum and tomato. Journal of virological methods, 236, 29-34.
  • Teber S., Ceylan A., Gürcan K., Candresse T., Ulubaş-Serçe Ç., Akbulut M., Akbaş B., 2019. Genetic diversity and molecular epidemiology of the T strain of Plum pox virus. Plant Pathology, 68(4), 755-763.
  • Teber S., Gürcan K., Brevet M., Palmisano F., Candresse T., Dallot S., 2023. High genetic diversity suggests a long evolutionary history of plum pox virus‐M in Turkey. Plant Pathology, 72(2), 300-311.
  • Thompson D., McCann M., MacLeod M., Lye D., Green M., James D., 2001. First report of plum pox potyvirus in Ontario, Canada. Plant Disease, 85(1), 97-97.
  • Varveri C., Ravelonandro M., Dunez J., 1987. Construction and use of a cloned cDNA probe for the detection of plum pox virus in plants. Phytopathology, 77(8), 1221-1224.
  • Varga A., and James D., 2006. Use of reverse transcription loop-mediated isothermal amplification for the detection of Plum pox virus. Journal of Virological Methods, 138(1-2), 184-190.
  • Zhao L. M., Li G., Gao Y., Zhu Y. R., Liu J., Zhu, X. P., 2015. Reverse transcription loop-mediated isothermal amplification assay for detecting tomato chlorosis virus. Journal of virological methods, 213, 93-97.
  • Zotto A. D., Ortego J. M., Raigón J. M., Caloggero S., Rossini M., Ducasse D. A., 2006. First report in Argentina of Plum pox virus causing sharka disease in Prunus. Plant Disease, 90(4), 523-523.
Toplam 54 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Tarımda Bitki Virolojisi
Bölüm Araştırma Makalesi
Yazarlar

Khalid Rasooly 0009-0007-0895-7316

Songül Yalçın Ateş 0000-0002-5514-594X

Burak Özgören 0000-0002-7969-9111

Ali Ferhan Morca 0000-0002-7480-922X

Ali Çelik 0000-0002-5836-8030

Yayımlanma Tarihi 23 Temmuz 2025
Gönderilme Tarihi 25 Mart 2025
Kabul Tarihi 22 Mayıs 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 12 Sayı: 3

Kaynak Göster

APA Rasooly, K., Yalçın Ateş, S., Özgören, B., Morca, A. F., vd. (2025). Performance of Ready-To-Use Isothermal Detection Kits in Plum pox virus (PPV) Detection. Turkish Journal of Agricultural and Natural Sciences, 12(3), 565-573. https://doi.org/10.30910/turkjans.1665625
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