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Preliminary Investigation of the Usability of Brown Meagre Otoliths as an Alternative Material in Bone Tissue Engineering

Yıl 2025, Cilt: 8 Sayı: 2, 94 - 102

Nermin Demirkol , Nihal Derin Coşkun

https://doi.org/10.34088/kojose.1591403

Öz

The need for biomaterials is increasing daily due to the increasing number of health problems and the doubling of the population. Especially bone structures are among the most important tissues in terms of human quality of life. In this study, Brown Meagre (BM) fish skull otoliths, which are organic-based calcite structures, were examined and a preliminary investigation of their usability for bone structures was carried out. For this purpose, otoliths taken from BMs speared from the Ordu coast of the Black Sea were cleaned with H2O2 and SEM, SEM EDX, XRD, FTIR and ICP MS analyses were performed, and the results were compared with bone structures. In the light of these data, it was determined by analyses that the calcite structure is in the aragonite phase and allows the accumulation of oligoelements. Otolith structures, which have the potential to be alternative raw materials for biomaterial production, also provide the utilisation of organic-based waste materials. It is aimed that otoliths, like coral structures studied in the literature, will contribute to bone tissue engineering studies, which are seen in calcite structures and whose biochemical interactions with enzyme structures are still not completely solved.

Anahtar Kelimeler

Biomaterial Brown Meagre Hydroxyapatite Material Characterization Otolith Tissue Engineering

Kaynakça

  • [1] Zahra D., Shokat Z., Ahmad A., Javaid A., Khurshid M., Ashfaq U.A., Nashwan A.J., 2023. Exploring the recent developments of alginate silk fibroin material for hydrogel wound dressing: A review. International Journal of Biological Macromolecules, 248, 125989, ISSN 0141-8130, https://doi.org/10.1016/j.ijbiomac.2023.125989.
  • [2] Uslu C., Tatar B.E., Uyanıkgil Y., Tomruk C., Yılmaz B., Demirkol N., Bozkurt, 2024. Evaluation of graphene oxide-doped polylactic-co-glycolic acid (GO-PLGA) nanofiber absorbable plates and titanium plates for bone stability and healing in mandibular corpus fractures: An experimental study. Journal of Plastic, Reconstructive & Aesthetic Surgery. 92, pp.79 – 86. https://doi.org/10.1016/j.bjps.2024.02.063.
  • [3] Kalmar L., Homola D., Varga G., and Tompa. P. 2012. Structural Disorder in Proteins Brings Order to Crystal Growth in Biomineralization. Bone, 51(3), pp. 528–34. https://doi.org/10.1016/j.bone.2012.05.009.
  • [4] Payan P., Pontual A.E.H.D., Borelli G., Boeuf G., and Gostan N.M. 1999. Chemical Composition of Saccular Endolymph and Otolith in Fish Inner Ear: Lack of Spatial Uniformity. American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 277(1), pp. 123–31. https://doi.org/10.1152/ajpregu.1999.277.1.r123.
  • [5] Olyveira G.M, Valido D.P., Costa L.M.M., Gois P.B.P., Filho L.X., and Basmaji P. 2011. First Otoliths/Collagen/Bacterial Cellulose Nanocomposites as a Potential Scaffold for Bone Tissue Regeneration. Journal of Biomaterials and Nanobiotechnology 02(03), pp. 239–43. https://doi.org/10.4236/jbnb.2011.23030.
  • [6] Montañez N.D., Carreño H., Escobar P., Estupiñán H.A., Peña D.Y., Goel S., and Endrino J.L. 2020. Functional Evaluation and Testing of a Newly Developed Teleost’s Fish Otolith Derived Biocomposite Coating for Healthcare. Scientific Reports. 10 (1), pp. 1–16. https://doi.org/10.1038/s41598-019-57128-w.
  • [7] Zhou Q., Su X., Wu J., Zhang X., Su R., Ma L., Sun Q., and He R., 2023. Additive Manufacturing of Bioceramic Implants for Restoration Bone Engineering: Technologies, Advances, and Future Perspectives. ACS Biomaterials Science and Engineering. 9 (3), pp. 1164–89. https://doi.org/10.1021/acsbiomaterials.2c01164.
  • [8] Zyoud W. A., Haddadin D., Hasan S.A., Jaradat H., and Kanoun O., 2023. Biocompatibility Testing for Implants: A Novel Tool for Selection and Characterization. Materials 16 (21). https://doi.org/10.3390/ma16216881
  • [9] Battat M., Omair N., WildAli M.A. et al. 2023. Factors associated with palliative care symptoms in cancer patients in Palestine. Sci Rep, 13, 16190. https://doi.org/10.1038/s41598-023-43469-0
  • [10] Miron R. J., 2023. Optimized Bone Grafting. Periodontology. 2000 no. July, pp. 143–60. https://doi.org/10.1111/prd.12517.
  • [11] Monika F., Detsch R., Horváth Z.E, Balázsi K., Boccaccini A. R., and Balázsi C., 2024. Amorphous, Carbonated Calcium Phosphate and Biopolymer-Composite-Coated Si3N4/MWCNTs as Potential Novel Implant Materials. Nanomaterials. 14 (3). https://doi.org/10.3390/nano14030279.
  • [12] Gomathy M., Paul A.J., and Krishnakumar V., 2024. A Systematic Review of Fish-Based Biomaterial on Wound Healing and Anti-Inflammatory Processes. Advances in Wound Care. 13 (2), pp. 83–96. https://doi.org/10.1089/wound.2022.0142.
  • [13] Xiangya H., Lou Y., Duan Y., Liu H., Tian J., Shen Y., and Wei X., 2024. Biomaterial Scaffolds in Maxillofacial Bone Tissue Engineering: A Review of Recent Advances. Bioactive Materials. 33 (October 2023): pp. 129–56. https://doi.org/10.1016/j.bioactmat.2023.10.031.
  • [14]https://www.scienceandthesea.org/program/200706/otoliths
  • [15] https://haberton.com/baligin-basindaki-otolit.
  • [16] İnsal B., and İlksin P., 2017. Kemik Dokusunun Fizyolojisi. Etlik Veteriner Mikrobiyoloji Dergisi. 28 (1), pp. 28–32. https://doi.org/10.35864/evmd.530089.
  • [17] Bodur B., Aydin M., and Karadurmuş U. 2023. Population Structure, Exploitation Status, and Prospects of Brown Meagre Sciaena Umbra Linnaeus, 1758 From the Turkish Coast of the Black Sea. Marine Science and Technology Bulletin. 2 (1), pp. 1–11. https://doi.org/10.33714/masteb.1178161.
  • [18] Sreedharan M., Vijayamma R., Liyaskina E., Revin, R.R. Ullah M.W., Shi Z., Yang G., et al. 2024. Nanocellulose-Based Hybrid Scaffolds for Skin and Bone Tissue Engineering: A 10-Year Overview. Biomacromolecules. 25 (4), pp. 2136–55. https://doi.org/10.1021/acs.biomac.3c00975.
  • [19] Aydın M., and Bengil, E.G.T., 2020. Feeding Habit and Length-Weight Relationship, Sciaena Umbra Linnaeus, 1758 from Southeastern Black Sea. Acta Aquatica Turcica 16 (4), pp. 479–86. https://doi.org/10.22392/actaquatr.714094.
  • [20] Bazin D., Chappard C., Combes C., Carpentier X., Rouzière S., André G., Matzen G., et al. 2009. Diffraction Techniques and Vibrational Spectroscopy Opportunities to Characterise Bones. Osteoporosis International. 20 (6), pp. 1065–75. https://doi.org/10.1007/s00198-009-0868-3.
  • [21] Manuela G.S., Zamora-Ledezma C., Elango J., and Morales-Flórez V., 2024. Novel Bioactive and Biocompatible Alumina-Wollastonite Porous Constructs Mimicking Physical Properties of Human Cortical Bone. Journal of the European Ceramic Society 44 (7), pp. 4699–4708. https://doi.org/10.1016/j.jeurceramsoc.2024.02.001.
  • [22] Habibovic P., Kruyt M.C., Juhl M.V., Clyens S., Martinetti R., Dolcini L., Theilgaard N., Blitterswijk C.Av., 2008. Comparative in vivo study of six hydroxyapatite-based bone graft substitutes. J Orthop Res; 26, pp. 1363–1370.
  • [23] Lafon, J. P., E., 2008. Champion, and D. Bernache-Assollant. Processing of AB-Type Carbonated Hydroxyapatite Ca10-x(PO4)6-x(CO3)x(OH)2-x-2y(CO3)y Ceramics with Controlled Composition. Journal of the European Ceramic Society. 28(1). https://doi.org/10.1016/j.jeurceramsoc.2007.06.009.
  • [24] Kapolos J., Koutsoukos P.G., 1999. Formation of calcium phosphates in aqueous solutions in the presence of carbonate ions. Langmuir, 15, pp.6557–6562, doi:10.1021/la981285k.
  • [25] Santos M., Gonzalez-Diaz P.F., 1977. A model for B carbonate apatite. Inorg Chem, 16, pp. 2131–2134.
  • [26] Hanan A., Redzuan M., Nasution A.K., Hussain R., and Saidin S., 2018. Fabrication of Poly(Lactic-Co-Glycolic Acid)/Calcium Phosphate Bone Cement Composite: Synthesization of Calcium Phosphate from Crab Shells. Jurnal Teknologi, 80 (4), pp. 103–9. https://doi.org/10.11113/jt.v80.11525.
  • [27] Ritesh K., and Mohanty S., 2022. Hydroxyapatite: A Versatile Bioceramic for Tissue Engineering Application. Journal of Inorganic and Organometallic Polymers and Materials, 32 (12), pp. 4461–77. https://doi.org/10.1007/s10904-022-02454-2.
  • [28] Leventouri, Th. A., Antonakos A., Kyriacou R., Liarokapis V.E., and Perdikatsis V., 2009. Crystal Structure Studies of Human Dental Apatite as a Function of Age. International Journal of Biomaterials, 2009(1), 698547, https://doi.org/10.1155/2009/698547.
  • [29] Carvalho D.C., Luciana M. C., Soledad M., Acevedo M.S.F., and Coelho N.M.M., 2015. The Oligoelements. Handbook of Mineral Elements in Food, pp. 109–22. https://doi.org/10.1002/9781118654316.ch5.

Preliminary Investigation of the Usability of Brown Meagre Otoliths as an Alternative Material in Bone Tissue Engineering

Yıl 2025, Cilt: 8 Sayı: 2, 94 - 102

Nermin Demirkol , Nihal Derin Coşkun

https://doi.org/10.34088/kojose.1591403

Öz

The need for biomaterials is increasing daily due to the increasing number of health problems and the doubling of the population. Especially bone structures are among the most important tissues in terms of human quality of life. In this study, Brown Meagre (BM) fish skull otoliths, which are organic-based calcite structures, were examined and a preliminary investigation of their usability for bone structures was carried out. For this purpose, otoliths taken from BMs speared from the Ordu coast of the Black Sea were cleaned with H2O2 and SEM, SEM EDX, XRD, FTIR and ICP MS analyses were performed, and the results were compared with bone structures. In the light of these data, it was determined by analyses that the calcite structure is in the aragonite phase and allows the accumulation of oligoelements. Otolith structures, which have the potential to be alternative raw materials for biomaterial production, also provide the utilisation of organic-based waste materials. It is aimed that otoliths, like coral structures studied in the literature, will contribute to bone tissue engineering studies, which are seen in calcite structures and whose biochemical interactions with enzyme structures are still not completely solved.

Anahtar Kelimeler

Biomaterials Brown Meagre Hydroxyapatite Material Characterization Otolith Tissue Engineering

Kaynakça

  • [1] Zahra D., Shokat Z., Ahmad A., Javaid A., Khurshid M., Ashfaq U.A., Nashwan A.J., 2023. Exploring the recent developments of alginate silk fibroin material for hydrogel wound dressing: A review. International Journal of Biological Macromolecules, 248, 125989, ISSN 0141-8130, https://doi.org/10.1016/j.ijbiomac.2023.125989.
  • [2] Uslu C., Tatar B.E., Uyanıkgil Y., Tomruk C., Yılmaz B., Demirkol N., Bozkurt, 2024. Evaluation of graphene oxide-doped polylactic-co-glycolic acid (GO-PLGA) nanofiber absorbable plates and titanium plates for bone stability and healing in mandibular corpus fractures: An experimental study. Journal of Plastic, Reconstructive & Aesthetic Surgery. 92, pp.79 – 86. https://doi.org/10.1016/j.bjps.2024.02.063.
  • [3] Kalmar L., Homola D., Varga G., and Tompa. P. 2012. Structural Disorder in Proteins Brings Order to Crystal Growth in Biomineralization. Bone, 51(3), pp. 528–34. https://doi.org/10.1016/j.bone.2012.05.009.
  • [4] Payan P., Pontual A.E.H.D., Borelli G., Boeuf G., and Gostan N.M. 1999. Chemical Composition of Saccular Endolymph and Otolith in Fish Inner Ear: Lack of Spatial Uniformity. American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 277(1), pp. 123–31. https://doi.org/10.1152/ajpregu.1999.277.1.r123.
  • [5] Olyveira G.M, Valido D.P., Costa L.M.M., Gois P.B.P., Filho L.X., and Basmaji P. 2011. First Otoliths/Collagen/Bacterial Cellulose Nanocomposites as a Potential Scaffold for Bone Tissue Regeneration. Journal of Biomaterials and Nanobiotechnology 02(03), pp. 239–43. https://doi.org/10.4236/jbnb.2011.23030.
  • [6] Montañez N.D., Carreño H., Escobar P., Estupiñán H.A., Peña D.Y., Goel S., and Endrino J.L. 2020. Functional Evaluation and Testing of a Newly Developed Teleost’s Fish Otolith Derived Biocomposite Coating for Healthcare. Scientific Reports. 10 (1), pp. 1–16. https://doi.org/10.1038/s41598-019-57128-w.
  • [7] Zhou Q., Su X., Wu J., Zhang X., Su R., Ma L., Sun Q., and He R., 2023. Additive Manufacturing of Bioceramic Implants for Restoration Bone Engineering: Technologies, Advances, and Future Perspectives. ACS Biomaterials Science and Engineering. 9 (3), pp. 1164–89. https://doi.org/10.1021/acsbiomaterials.2c01164.
  • [8] Zyoud W. A., Haddadin D., Hasan S.A., Jaradat H., and Kanoun O., 2023. Biocompatibility Testing for Implants: A Novel Tool for Selection and Characterization. Materials 16 (21). https://doi.org/10.3390/ma16216881
  • [9] Battat M., Omair N., WildAli M.A. et al. 2023. Factors associated with palliative care symptoms in cancer patients in Palestine. Sci Rep, 13, 16190. https://doi.org/10.1038/s41598-023-43469-0
  • [10] Miron R. J., 2023. Optimized Bone Grafting. Periodontology. 2000 no. July, pp. 143–60. https://doi.org/10.1111/prd.12517.
  • [11] Monika F., Detsch R., Horváth Z.E, Balázsi K., Boccaccini A. R., and Balázsi C., 2024. Amorphous, Carbonated Calcium Phosphate and Biopolymer-Composite-Coated Si3N4/MWCNTs as Potential Novel Implant Materials. Nanomaterials. 14 (3). https://doi.org/10.3390/nano14030279.
  • [12] Gomathy M., Paul A.J., and Krishnakumar V., 2024. A Systematic Review of Fish-Based Biomaterial on Wound Healing and Anti-Inflammatory Processes. Advances in Wound Care. 13 (2), pp. 83–96. https://doi.org/10.1089/wound.2022.0142.
  • [13] Xiangya H., Lou Y., Duan Y., Liu H., Tian J., Shen Y., and Wei X., 2024. Biomaterial Scaffolds in Maxillofacial Bone Tissue Engineering: A Review of Recent Advances. Bioactive Materials. 33 (October 2023): pp. 129–56. https://doi.org/10.1016/j.bioactmat.2023.10.031.
  • [14]https://www.scienceandthesea.org/program/200706/otoliths
  • [15] https://haberton.com/baligin-basindaki-otolit.
  • [16] İnsal B., and İlksin P., 2017. Kemik Dokusunun Fizyolojisi. Etlik Veteriner Mikrobiyoloji Dergisi. 28 (1), pp. 28–32. https://doi.org/10.35864/evmd.530089.
  • [17] Bodur B., Aydin M., and Karadurmuş U. 2023. Population Structure, Exploitation Status, and Prospects of Brown Meagre Sciaena Umbra Linnaeus, 1758 From the Turkish Coast of the Black Sea. Marine Science and Technology Bulletin. 2 (1), pp. 1–11. https://doi.org/10.33714/masteb.1178161.
  • [18] Sreedharan M., Vijayamma R., Liyaskina E., Revin, R.R. Ullah M.W., Shi Z., Yang G., et al. 2024. Nanocellulose-Based Hybrid Scaffolds for Skin and Bone Tissue Engineering: A 10-Year Overview. Biomacromolecules. 25 (4), pp. 2136–55. https://doi.org/10.1021/acs.biomac.3c00975.
  • [19] Aydın M., and Bengil, E.G.T., 2020. Feeding Habit and Length-Weight Relationship, Sciaena Umbra Linnaeus, 1758 from Southeastern Black Sea. Acta Aquatica Turcica 16 (4), pp. 479–86. https://doi.org/10.22392/actaquatr.714094.
  • [20] Bazin D., Chappard C., Combes C., Carpentier X., Rouzière S., André G., Matzen G., et al. 2009. Diffraction Techniques and Vibrational Spectroscopy Opportunities to Characterise Bones. Osteoporosis International. 20 (6), pp. 1065–75. https://doi.org/10.1007/s00198-009-0868-3.
  • [21] Manuela G.S., Zamora-Ledezma C., Elango J., and Morales-Flórez V., 2024. Novel Bioactive and Biocompatible Alumina-Wollastonite Porous Constructs Mimicking Physical Properties of Human Cortical Bone. Journal of the European Ceramic Society 44 (7), pp. 4699–4708. https://doi.org/10.1016/j.jeurceramsoc.2024.02.001.
  • [22] Habibovic P., Kruyt M.C., Juhl M.V., Clyens S., Martinetti R., Dolcini L., Theilgaard N., Blitterswijk C.Av., 2008. Comparative in vivo study of six hydroxyapatite-based bone graft substitutes. J Orthop Res; 26, pp. 1363–1370.
  • [23] Lafon, J. P., E., 2008. Champion, and D. Bernache-Assollant. Processing of AB-Type Carbonated Hydroxyapatite Ca10-x(PO4)6-x(CO3)x(OH)2-x-2y(CO3)y Ceramics with Controlled Composition. Journal of the European Ceramic Society. 28(1). https://doi.org/10.1016/j.jeurceramsoc.2007.06.009.
  • [24] Kapolos J., Koutsoukos P.G., 1999. Formation of calcium phosphates in aqueous solutions in the presence of carbonate ions. Langmuir, 15, pp.6557–6562, doi:10.1021/la981285k.
  • [25] Santos M., Gonzalez-Diaz P.F., 1977. A model for B carbonate apatite. Inorg Chem, 16, pp. 2131–2134.
  • [26] Hanan A., Redzuan M., Nasution A.K., Hussain R., and Saidin S., 2018. Fabrication of Poly(Lactic-Co-Glycolic Acid)/Calcium Phosphate Bone Cement Composite: Synthesization of Calcium Phosphate from Crab Shells. Jurnal Teknologi, 80 (4), pp. 103–9. https://doi.org/10.11113/jt.v80.11525.
  • [27] Ritesh K., and Mohanty S., 2022. Hydroxyapatite: A Versatile Bioceramic for Tissue Engineering Application. Journal of Inorganic and Organometallic Polymers and Materials, 32 (12), pp. 4461–77. https://doi.org/10.1007/s10904-022-02454-2.
  • [28] Leventouri, Th. A., Antonakos A., Kyriacou R., Liarokapis V.E., and Perdikatsis V., 2009. Crystal Structure Studies of Human Dental Apatite as a Function of Age. International Journal of Biomaterials, 2009(1), 698547, https://doi.org/10.1155/2009/698547.
  • [29] Carvalho D.C., Luciana M. C., Soledad M., Acevedo M.S.F., and Coelho N.M.M., 2015. The Oligoelements. Handbook of Mineral Elements in Food, pp. 109–22. https://doi.org/10.1002/9781118654316.ch5.
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Malzeme Karekterizasyonu, Malzeme Mühendisliğinde Seramik
Bölüm Makaleler
Yazarlar

Nermin Demirkol 0000-0001-9088-023X

Nihal Derin Coşkun 0000-0002-3024-9443

Erken Görünüm Tarihi 28 Haziran 2025
Yayımlanma Tarihi
Gönderilme Tarihi 26 Kasım 2024
Kabul Tarihi 30 Aralık 2024
Yayımlandığı Sayı Yıl 2025 Cilt: 8 Sayı: 2

Kaynak Göster

APA Demirkol, N., & Derin Coşkun, N. (t.y.). Preliminary Investigation of the Usability of Brown Meagre Otoliths as an Alternative Material in Bone Tissue Engineering. Kocaeli Journal of Science and Engineering, 8(2), 94-102. https://doi.org/10.34088/kojose.1591403