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Yıl 2025, Cilt: 9 Sayı: 2, 570 - 576, 26.06.2025

Gökhan Ovalıoglu Yeter Çilesiz

https://doi.org/10.31015/2025.2.30

Öz

Proje Numarası

2025-YLTP-TBT-0001

Kaynakça

  • Bernard, A., Barreneche, T., Lheureux, F., Dirlewanger, E. (2018). Analysis of genetic diversity and structure in a worldwide walnut (Juglans regia L.) germplasm using SSR markers. Plos One, 13(11), e0208021. https://doi.org/10.1371/journal.pone.0208021
  • Collard, B.C., Mackill, D.J. (2009). Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Molecular Biology Reporter, 27, 86-93. https://doi.org/10.1007/s11105-008-0060-5
  • Delaviz, H., Mohammadi, J., Ghalamfarsa, G., Mohammadi, B., Farhadi, N. (2017). A review study on phytochemistry and pharmacology applications of Juglans regia plant. Pharmacognosy Reviews, 11(22), 145. https://doi.org/10.4103/phrev.phrev_10_17
  • Doyle, J.J., Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13-15.
  • El-Sheikh, M.H., Ghareeb, R.Y., Ibrahim, A.A., Hafez, E.E. (2020). Genetic diversity among Juglans regia and Prunus dulcis genotypes using RAPD and ISSR molecular markers. Plant Archives, 20(2), 9693-9700.
  • Guney, M., Kafkas, S., Keles, H., Zarifikhosroshahi, M., Gundesli, M. A., Ercisli, S., Bujdoso, G. (2021). Genetic diversity among some walnut (Juglans regia L.) genotypes by SSR markers. Sustainability, 13(12), 6830. https://doi.org/10.3390/su13126830
  • Hayes, D., Angove, M.J., Tucci, J., Dennis, C. (2016). Walnuts (Juglans regia) chemical composition and research in human health. Critical Reviews in Food Science and Nutrition, 56(8), 1231-1241. https://doi.org/10.1080/10408398.2012.760516
  • Jalilian, H., Zarei, A., Erfani-Moghadam, J. (2018). Phylogeny relationship among commercial and wild pear species based on morphological characteristics and SCoT molecular markers. Scientia Horticulturae, 235, 323-333. https://doi.org/10.1016/j.scienta.2018.03.020
  • Kovach, W.L. (2007) MVSP-A MultiVariate Statistical Package for Windows, ver. 3.1. Kovach Computing Services, Pentraeth Wales, UK.
  • Martínez, M.L., Labuckas, D.O., Lamarque, A.L., Maestri, D.M. (2010). Walnut (Juglans regia L.): genetic resources, chemistry, by‐products. Journal of the Science of Food and Agriculture, 90(12), 1959-1967. https://doi.org/10.1002/jsfa.4059
  • Mishra, S.P., Sarkar, U., Taraphder, S., Datta, S., Swain, D., Saikhom, R., Laishram, M., (2017). Multivariate statistical data analysis—principal component analysis (PCA), International Journal of Livestock Research, 7, 60–78. https://doi.org/10.5455/ijlr.20170415115235
  • Mohammadi, C., Seyedi, N., Mandoulakani, B.A., Caeiro, M.F. (2021). Genetic diversity in natural populations and cultivated stands of walnut (Juglans regia L.) using microsatellite marker. Brazilian Archives of Biology and Technology, 64, e21200745. https://doi.org/10.1590/1678-4324-2021200745
  • Nachimuthu, V.V., Robin, S., Sudhakar, D., Raveendran, M., Rajeswari, S., Manonmani, S. (2014). Evaluation of rice genetic diversity and variability in a population panel by principal component analysis. Indian journal of Science and Technology, 7(10), 1555-1562.
  • Pan, H., Deng, L., Zhu, K., Shi, D., Wang, F., Cui, G. (2024). Evaluation of genetic diversity and population structure of Annamocarya sinensis using SCoT markers. PloS One, 19(9), e0309283. https://doi.org/10.1371/journal.pone.0309283
  • Sağbaş, H., Ercişli, S., Aydin, M., Ilhan, E., Aydinyurt, R., Kasapoglu, A., Polat, Y. (2023). Evaluation of genetic diversity using iPBS-SCoT marker methods in native hawthorn genetic resources and species ıdentification by using DNA barcoding method. Cellular and molecular biology (Noisy-le-Grand, France), 69(10).
  • Seresht, E.S., Karimi, H.R., Malekzadeh, K., Mirdehghan, S.H., Mirik, A.A.M. (2023). Genetic structure analysis and genetic fingerprinting of pomegranate cultivars (Punica granatum L.) by using SCoT molecular markers. Plant Genetic Resources, 21(1), 37-44. https://doi.org/10.1017/S1479262123000394
  • Shah, R.A., Bakshi, P., Jasrotia, A., Itoo, H., Padder, B. A., Gupta, R., Dolkar, D. (2023). Morphological to molecular markers: Plant genetic diversity studies in walnut (Juglans regia L.) - A Review. Erwerbs-Obstbau, 65(5), 1499-1511. https://doi.org/10.1007/s10341-023-00892-x
  • Tabasi, M., Sheidai, M., Hassani, D., Koohdar, F. (2020). DNA fingerprinting and genetic diversity analysis with SCoT markers of Persian walnut populations (Juglans regia L.) in Iran. Genetic Resources and Crop Evolution, 67, 1437-1447. https://doi.org/10.1007/s10722-020-00914-7
  • Tenche-Constantinescu, A.M., Lalescu, D.V., Popescu, S., Sarac, I., Petolescu, C., Camen, D., Onisan, E. (2024). Juglans regia as urban trees: genetic diversity and walnut kernel quality assessment. Horticulturae, 10(10), 1027. https://doi.org/10.3390/horticulturae10101027
  • Vischi, M., Chiabà, C., Raranciuc, S., Poggetti, L., Messina, R., Ermacora, P., Testolin, R. (2017). Genetic diversity of walnut (Juglans regia L.) in the Eastern Italian Alps. Forests, 8(3), 81. https://doi.org/10.3390/f8030081
  • Wani, M.S., Hussain, A., Ganie, S.A., Munshi, A.H., Lal, E.P., Gupta, R.C. (2016). Juglans regia-A review. International Journal of Latest Research in Science and Technology, 5, 90-97.
  • Xie, Q., Liu, B., Wang, X., Wu, H., Du, J., Liu, Z. (2025). Assessment of genetic diversity and population structure of common walnut (Juglans regia) germplasm with simple sequence repeat (SSR) markers. Genetic Resources and Crop Evolution, 72(2), 2265-2276. https://doi.org/10.1007/s10722-024-02096-y
  • Yıldız, E., Pinar, H., Uzun, A., Yaman, M., Sumbul, A., Ercisli, S. (2021). Identification of genetic diversity among Juglans regia L. genotypes using molecular, morphological and fatty acid data. Genetic Resources and Crop Evolution, 68, 1425-1437. https://doi.org/10.1007/s10722-020-01072-6
  • Zarinkolah, M., Karimi, H.R., Esmaeilzadeh, M., Malekzadeh, K., Sahhafi, S.R. (2025). Genetic relationships among some old walnuts (Juglans regia L.) genotypes in Iran and selection of superior genotypes. Plant Genetic Resources, 23(2), 106-119. https://doi.org/10.1017/S1479262124000583
  • Zarinkolah, M., Karimi, H.R., Esmaeilzadeh, M., Malekzadeh, K., Sahhafi, S.R. (2024). Study of association between nut traits and SCoT molecular marker in some old iranian walnut trees. Plant Molecular Biology Reporter, 1-13. https://doi.org/10.1007/s11105-024-01524-8
  • Zhang, S., Li, Y., Li, Y., Zhang, Y., Hao, Y., Hou, Z., Qi, J. (2024). Development of SSR markers for and fingerprinting of walnut genetic resources. Forests, 15(3), 405. https://doi.org/10.3390/f15030405

SCoT marker-based evaluation of genetic diversity in selected walnut (Juglans regia L.) accessions

Yıl 2025, Cilt: 9 Sayı: 2, 570 - 576, 26.06.2025

Gökhan Ovalıoglu Yeter Çilesiz

https://doi.org/10.31015/2025.2.30

Öz

This study investigates the genetic diversity among different walnut (Juglans regia L.) genotypes using the SCoT (Start Codon Targeted) marker system. A total of 136 bands were obtained from 15 markers, of which 106 were polymorphic, with an average of 9.07 bands per primer. The SCoT markers yielded a polymorphism rate of 77.9%. In order to interpret the genetic distance among Juglans regia L. genotypes, a UPGMA dendrogram was constructed using MVSP 3.22 software. According to the resulting dendrogram, the lowest similarity (0.593) was observed between samples W6 and W26, while the highest similarity (0.970) was observed between W1 and W4, followed by 0.962 between W4 and W5. Principal Component Analysis (PCA) was also conducted using MVSP 3.22. The PCA results revealed a homogeneous distribution and wide variation. The UPGMA dendrogram and PCA analysis were consistent with each other. The SCoT analyses conducted on walnut genotypes provide highly valuable information for assessing the level of genetic diversity, understanding population structure and selecting superior individuals. Such studies contribute to the conservation of genetic resources and support the development of new cultivars with high yield, disease resistance and strong adaptation to climate change in future breeding programs. Based on this study, SCoT analyses demonstrate that the Sivas province constitutes an important genetic diversity reservoir and that walnut genotypes possess rich genetic variation.

Anahtar Kelimeler

Juglans regia L. SCoT marker Genetic diversity

Destekleyen Kurum

Sivas Science and Technology University Scientific Research Projects Coordination

Proje Numarası

2025-YLTP-TBT-0001

Teşekkür

This study was produced from the project number 2025-YLTP-TBT-0001 (Determination of genetic diversity in some walnut genotypes (Juglans regia L.) using start codon-targeted (SCoT) markers), which was carried out with the support of Sivas Science and Technology University Scientific Research Projects Coordination. This study was derived from the first author's master's thesis titled "Determination of Genetic Diversity in Some Walnut Genotypes (Juglans regia L.) Using Start Codon Targeted (SCoT) Markers."

Kaynakça

  • Bernard, A., Barreneche, T., Lheureux, F., Dirlewanger, E. (2018). Analysis of genetic diversity and structure in a worldwide walnut (Juglans regia L.) germplasm using SSR markers. Plos One, 13(11), e0208021. https://doi.org/10.1371/journal.pone.0208021
  • Collard, B.C., Mackill, D.J. (2009). Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Molecular Biology Reporter, 27, 86-93. https://doi.org/10.1007/s11105-008-0060-5
  • Delaviz, H., Mohammadi, J., Ghalamfarsa, G., Mohammadi, B., Farhadi, N. (2017). A review study on phytochemistry and pharmacology applications of Juglans regia plant. Pharmacognosy Reviews, 11(22), 145. https://doi.org/10.4103/phrev.phrev_10_17
  • Doyle, J.J., Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13-15.
  • El-Sheikh, M.H., Ghareeb, R.Y., Ibrahim, A.A., Hafez, E.E. (2020). Genetic diversity among Juglans regia and Prunus dulcis genotypes using RAPD and ISSR molecular markers. Plant Archives, 20(2), 9693-9700.
  • Guney, M., Kafkas, S., Keles, H., Zarifikhosroshahi, M., Gundesli, M. A., Ercisli, S., Bujdoso, G. (2021). Genetic diversity among some walnut (Juglans regia L.) genotypes by SSR markers. Sustainability, 13(12), 6830. https://doi.org/10.3390/su13126830
  • Hayes, D., Angove, M.J., Tucci, J., Dennis, C. (2016). Walnuts (Juglans regia) chemical composition and research in human health. Critical Reviews in Food Science and Nutrition, 56(8), 1231-1241. https://doi.org/10.1080/10408398.2012.760516
  • Jalilian, H., Zarei, A., Erfani-Moghadam, J. (2018). Phylogeny relationship among commercial and wild pear species based on morphological characteristics and SCoT molecular markers. Scientia Horticulturae, 235, 323-333. https://doi.org/10.1016/j.scienta.2018.03.020
  • Kovach, W.L. (2007) MVSP-A MultiVariate Statistical Package for Windows, ver. 3.1. Kovach Computing Services, Pentraeth Wales, UK.
  • Martínez, M.L., Labuckas, D.O., Lamarque, A.L., Maestri, D.M. (2010). Walnut (Juglans regia L.): genetic resources, chemistry, by‐products. Journal of the Science of Food and Agriculture, 90(12), 1959-1967. https://doi.org/10.1002/jsfa.4059
  • Mishra, S.P., Sarkar, U., Taraphder, S., Datta, S., Swain, D., Saikhom, R., Laishram, M., (2017). Multivariate statistical data analysis—principal component analysis (PCA), International Journal of Livestock Research, 7, 60–78. https://doi.org/10.5455/ijlr.20170415115235
  • Mohammadi, C., Seyedi, N., Mandoulakani, B.A., Caeiro, M.F. (2021). Genetic diversity in natural populations and cultivated stands of walnut (Juglans regia L.) using microsatellite marker. Brazilian Archives of Biology and Technology, 64, e21200745. https://doi.org/10.1590/1678-4324-2021200745
  • Nachimuthu, V.V., Robin, S., Sudhakar, D., Raveendran, M., Rajeswari, S., Manonmani, S. (2014). Evaluation of rice genetic diversity and variability in a population panel by principal component analysis. Indian journal of Science and Technology, 7(10), 1555-1562.
  • Pan, H., Deng, L., Zhu, K., Shi, D., Wang, F., Cui, G. (2024). Evaluation of genetic diversity and population structure of Annamocarya sinensis using SCoT markers. PloS One, 19(9), e0309283. https://doi.org/10.1371/journal.pone.0309283
  • Sağbaş, H., Ercişli, S., Aydin, M., Ilhan, E., Aydinyurt, R., Kasapoglu, A., Polat, Y. (2023). Evaluation of genetic diversity using iPBS-SCoT marker methods in native hawthorn genetic resources and species ıdentification by using DNA barcoding method. Cellular and molecular biology (Noisy-le-Grand, France), 69(10).
  • Seresht, E.S., Karimi, H.R., Malekzadeh, K., Mirdehghan, S.H., Mirik, A.A.M. (2023). Genetic structure analysis and genetic fingerprinting of pomegranate cultivars (Punica granatum L.) by using SCoT molecular markers. Plant Genetic Resources, 21(1), 37-44. https://doi.org/10.1017/S1479262123000394
  • Shah, R.A., Bakshi, P., Jasrotia, A., Itoo, H., Padder, B. A., Gupta, R., Dolkar, D. (2023). Morphological to molecular markers: Plant genetic diversity studies in walnut (Juglans regia L.) - A Review. Erwerbs-Obstbau, 65(5), 1499-1511. https://doi.org/10.1007/s10341-023-00892-x
  • Tabasi, M., Sheidai, M., Hassani, D., Koohdar, F. (2020). DNA fingerprinting and genetic diversity analysis with SCoT markers of Persian walnut populations (Juglans regia L.) in Iran. Genetic Resources and Crop Evolution, 67, 1437-1447. https://doi.org/10.1007/s10722-020-00914-7
  • Tenche-Constantinescu, A.M., Lalescu, D.V., Popescu, S., Sarac, I., Petolescu, C., Camen, D., Onisan, E. (2024). Juglans regia as urban trees: genetic diversity and walnut kernel quality assessment. Horticulturae, 10(10), 1027. https://doi.org/10.3390/horticulturae10101027
  • Vischi, M., Chiabà, C., Raranciuc, S., Poggetti, L., Messina, R., Ermacora, P., Testolin, R. (2017). Genetic diversity of walnut (Juglans regia L.) in the Eastern Italian Alps. Forests, 8(3), 81. https://doi.org/10.3390/f8030081
  • Wani, M.S., Hussain, A., Ganie, S.A., Munshi, A.H., Lal, E.P., Gupta, R.C. (2016). Juglans regia-A review. International Journal of Latest Research in Science and Technology, 5, 90-97.
  • Xie, Q., Liu, B., Wang, X., Wu, H., Du, J., Liu, Z. (2025). Assessment of genetic diversity and population structure of common walnut (Juglans regia) germplasm with simple sequence repeat (SSR) markers. Genetic Resources and Crop Evolution, 72(2), 2265-2276. https://doi.org/10.1007/s10722-024-02096-y
  • Yıldız, E., Pinar, H., Uzun, A., Yaman, M., Sumbul, A., Ercisli, S. (2021). Identification of genetic diversity among Juglans regia L. genotypes using molecular, morphological and fatty acid data. Genetic Resources and Crop Evolution, 68, 1425-1437. https://doi.org/10.1007/s10722-020-01072-6
  • Zarinkolah, M., Karimi, H.R., Esmaeilzadeh, M., Malekzadeh, K., Sahhafi, S.R. (2025). Genetic relationships among some old walnuts (Juglans regia L.) genotypes in Iran and selection of superior genotypes. Plant Genetic Resources, 23(2), 106-119. https://doi.org/10.1017/S1479262124000583
  • Zarinkolah, M., Karimi, H.R., Esmaeilzadeh, M., Malekzadeh, K., Sahhafi, S.R. (2024). Study of association between nut traits and SCoT molecular marker in some old iranian walnut trees. Plant Molecular Biology Reporter, 1-13. https://doi.org/10.1007/s11105-024-01524-8
  • Zhang, S., Li, Y., Li, Y., Zhang, Y., Hao, Y., Hou, Z., Qi, J. (2024). Development of SSR markers for and fingerprinting of walnut genetic resources. Forests, 15(3), 405. https://doi.org/10.3390/f15030405
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Gıda Biyoteknolojisi, Tarımsal Biyoteknoloji (Diğer)
Bölüm Makaleler
Yazarlar

Gökhan Ovalıoglu 0009-0004-6235-8435

Yeter Çilesiz 0000-0002-4313-352X

Proje Numarası 2025-YLTP-TBT-0001
Yayımlanma Tarihi 26 Haziran 2025
Gönderilme Tarihi 27 Nisan 2025
Kabul Tarihi 7 Haziran 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 9 Sayı: 2

Kaynak Göster

APA Ovalıoglu, G., & Çilesiz, Y. (2025). SCoT marker-based evaluation of genetic diversity in selected walnut (Juglans regia L.) accessions. International Journal of Agriculture Environment and Food Sciences, 9(2), 570-576. https://doi.org/10.31015/2025.2.30
 Kapak Resmi İndir


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